Differential Endothelial Adhesion Molecule Expression in Early and Late Whealing Reactions

Haas, Norbert; Schadendorf, Dirk; Henz, Beate M.

doi:10.1159/000023902

Cited by 53 publications

(46 citation statements)

References 15 publications

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“…When the comparison was made with unaffected skin, no significant differences could be detected for VCAM and ICAM, even if a slight increase was observed in lesional skin. These last data are in agreement with those of Haas (34), who could not detect any increase of CAMs in early whealing reactions, and seem to confirm for VCAM and ICAM‐1 the systemic activation suggested earlier for mast cells and lymphocytes.…”

Section: Discussionsupporting

confidence: 92%

Infiltrating cells and related cytokines in lesional skin of patients with chronic idiopathic urticaria and positive autologous serum skin test

Caproni¹,

Volpi²,

Macchia

et al. 2003

Experimental Dermatology

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In approximately one-third of patients with chronic idiopathic urticaria (CIU), autoantibodies against the high-affinity IgE receptor and/ or against IgE can be detected and a wheal-and-flare response can be provoked by the intradermal injection of autologous serum (ASST). In this study we aimed to further characterize the inflammatory response observed in the subgroup of CIU patients with positive ASST and serum-evoked histamine-release in vitro from basophils in comparison with unaffected skin and healthy donors. An immunohistochemical analysis of infiltrating cells (CD4, MPO, EG1, EG2, tryptase), cytokines (IL-4, IL-5, IFN-gamma), chemokines and chemokine receptors (IL-8, CCR3, CXCR3), and adhesion molecules (ICAM-1, VCAM-1, ELAM-1) was performed on seven selected patients (four males and three females; median age: 45 years; range: 22-57) and five healthy donors. Cytokine evaluation was also performed in five psoriatic patients to obtain an additional control. In spontaneous wheals we observed an increased number of CD4+ T lymphocytes when compared with the controls, and an increased number of neutrophils and eosinophils, whereas mast cells did not show a significant variation. A significant expression for IL-4 and IL-5 could only be observed in lesional skin, while IFN-gamma showed a slight expression in the same site. Chemokine receptors CCR3 and CXCR3 did not show a defined polarized response in either lesional or unaffected skin. An increased expression of all cellular adhesion molecules (CAMs) studied was detected in spontaneous wheals. The lack of a significant difference in the expression of tryptase + mast cells, T lymphocytes, IL-8, CXCR3 and CCR3, a few CAMs between the lesional and unaffected skin of CIU patients suggests a wide immunological activation that involves not only lesional tissues, but possibly extends to the whole of the skin's immune system.

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Section: Discussionsupporting

confidence: 92%

Infiltrating cells and related cytokines in lesional skin of patients with chronic idiopathic urticaria and positive autologous serum skin test

Caproni¹,

Volpi²,

Macchia

et al. 2003

Experimental Dermatology

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“…Some studies have demonstrated that ELAM-I, ICAM-l and YCAM-l are increased in spontaneous wheals of patients with CIU (33). Other experiences carried out in patients with chronic recurrent urticaria have demonstrated an upregulation ofELAM-I, ICAM-I and p2-integrins in non-lesional skin (23)(24). This study has confirmed the presence of adhesion molecules in non-Iesional skin of many cases, suggesting that endothelial cells are activated also during the apparent absence of clinical signs.…”

Section: Discussionmentioning

confidence: 99%

Fexofenadine in Chronic Idiopathic Urticaria: A Clinical and Immunohistochemical Evaluation

Cassano

Filieri

et al. 2002

Int J Immunopathol Pharmacol

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Fexofenadine is a non-sedating selective third-generation antihistamine, which also exerts an antiinflammatory action. The aim of this study was to evaluate the influence on the expression of inflammatory skin mediators, together with the efficacy and tolerability, of fexofenadine in chronic idiopathic urticaria (CIU). Fexofenadine 180mg was administered once daily for 4 weeks after a placebo run-in phase of3 to 7 days. Efficacy paramaters were obtained from patients' assessment of urticaria symptoms. Non-Iesional skin of patients with active CIU was studied immunohistochemically before and after treatment. The expression of the following mediators was evaluated: adhesion molecules (ICAM-l, ELAM-l, VCAM-l)j mast cell proteases (chymase and tryptase) and proinflammatory cytokines (IL-l~, IL-3, IL-6 and TNF-n). Of the 20 subjects enrolled, 3 dropped out of the study. Treatment proved successful in most cases (88.2 %) (p <0.01) and a significant improvement of all symptoms was registered. Treatment was well-tolerated by all patients; adverse events, neither serious nor drug-related, occurred in any case. Immunochemistry revealed at the baseline a significant expression of ELAM-l, VCAM-l, tryptase, chymase, and TNF-« (p~0.05) in non-Iesional skin of patients compared to normal controls. After treatment with fexofenadine, there was a significant decrease in the expression of ELAM-l (p= 0.02), VCAM-l (p= 0.04) and tryptase (p=0.04), whereas no relevant change was observed for the other parameters examined. This work confirms the efficacy and tolerability of fexofenadine HCI 180mg in CIU. These preliminary data show a trend towards a decrease in the expression of tryptase and some adhesion molecules after treatment, suggesting an anti-inflammatory activity of fexofenadine.Chronic idiopathic urticaria (CIU) is defined as the occurrence of wheals on most days for more than six weeks in the absence of any known causative or triggering agents (1). As it is not possible to adopt specific measures of prevention in CIU, treatment hangs upon symptomatic tools, with HI antihistamines as the mainstay for patients with uncomplicated forms (2-3). Newgeneration antihistamines are usually preferred owing to the greater affinity for H 1 receptors and the lower incidence of sedation and anticholinergic effects compared with older molecules (4-5).Fexofenadine is a non-sedating third generation antihistamine with highly selective H 1-receptor antagonist activity, which has been approved for the treatment of seasonal allergic rhinitis and CIU. Controlled studies have proved the high therapeutic value and the excellent tolerability offexofenadine (6-11). The good safety profile of fexofenadine is also due to the absence of cardiotoxic effects and sedation, even at doses much higher than those recommended for aller- gic rhinitis and CIU. Moreover, fexofenadine undergoes minimal systemic metabolism and is well tolerated, also in patients with renal or hepatic impairment and in the elderly. It appears to be associated with no risk of signifi...

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“…Na obszarze skóry, w obrębie której rozwijają się bąble pokrzywkowe, prawie zawsze dochodzi do zwiększenia ekspresji cząsteczek przylegania komór kowego w śródbłonku oraz powstania okołonaczy niowego mieszanego nacieku zapalnego. Nasilenie stanu zapalnego bywa zmienne, a skład komórkowy obejmuje neutrofile, eozynofile, makrofagi oraz lim focyty T. Nie stwierdza się natomiast martwicy ściany naczyń, co jest charakterystyczne dla pokrzywki na czyniowej (urticaria vasculitis) [12][13][14]. Niektórzy au torzy donoszą także o łagodnym do umiarkowanego zwiększeniu liczby komórek tucznych.…”

Section: Aspekty Patofizjologiczneunclassified

The EAACI/GA2LEN/EDF/WAO Guideline for the definition, classification, diagnosis, and management of urticaria: the 2013 revision and update

Zuberbier¹,

Aberer²,

Asero³

et al. 2015

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Differential Endothelial Adhesion Molecule Expression in Early and Late Whealing Reactions

Cited by 53 publications

References 15 publications

Infiltrating cells and related cytokines in lesional skin of patients with chronic idiopathic urticaria and positive autologous serum skin test

Infiltrating cells and related cytokines in lesional skin of patients with chronic idiopathic urticaria and positive autologous serum skin test

Fexofenadine in Chronic Idiopathic Urticaria: A Clinical and Immunohistochemical Evaluation

The EAACI/GA2LEN/EDF/WAO Guideline for the definition, classification, diagnosis, and management of urticaria: the 2013 revision and update

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