Differential Effects of Transition Metals on Growth and Metal Uptake for Two Distinct<i>Lactobacillus</i>Species

Huynh, Uyen; Qiao, Muxin; King, John W.; Trinh, Brittany; Valdez, Juventino; Haq, Marium; Zastrow, Melissa L.

doi:10.1128/spectrum.01006-21

Cited by 16 publications

(11 citation statements)

References 133 publications

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“…Even for MRS (control), the specific speeds of 0,15 h -1 and generation time of 1,37 h described have been exceeded (Śliżewska & Chlebicz-Wójcik, 2020). In previous studies, specific speeds ranging from 0,23 to 0,73 h -1 and latency times from 2,89 to 4,4 h have been reported (Hang et al, 2020;Huynh et al, 2022;Rocha-Mendoza et al, 2020). In these studies, the differences in the parameters have been attributed to the availability of sugars in culture media (Alemneh et al, 2021).…”

Section: Effect Of Ae On the Growth Of L Plantarum And L Acidophilusmentioning

confidence: 81%

“…L. plantarum exhibited a velocity of 0,136 h -1 at 35 °C indicating that the specific speed decreases when the temperature varies (Canci et al, 2022). On the other hand, although for L. acidophilus there was no improvement in kinetic parameters with AEs, the values in MRS were comparable with latency times of 3,4 to 4 h and speeds of 0,25 to 0,29 h -1 (Huynh et al, 2022). Other studies have reported a specific rate for MRS from 0,05 to 0,11 h -1 and a latency time from 3,14 to 5,3 h (Kolev et al, 2022).…”

Section: Effect Of Ae On the Growth Of L Plantarum And L Acidophilusmentioning

confidence: 92%

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Potencial prebiótico in vitro del extracto atomizado de Swartzia polyphylla, Maytenus macrocarpa y Jatropha macrantha sobre Lactobacillus plantarum y Lactobacillus acidophilus

HUAPAYA¹,

Zavaleta²,

RAMIREZ³

et al. 2022

Manglar

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The combination of prebiotics stimulates the growth of intestinal biota. Atomized extracts containing phenolic compounds promote the proliferation of some lactic acid bacteria (LAB). The objective of this research was to determine in vitro prebiotic potential of the atomized extract (AE) of Swartzia polyphylla (Cumaceba), Maytenus macrocarpa (Chuchuwasi) and Jatropha macrantha (Huanarpo male) on L. plantarum ATCC 14917 and L. acidophilus ATCC 4356. To do this, the bacteria were grown in the De Man Rogosa Sharp (MRS) media containing 1 and 2% (w/v) of AE in anaerobiosis at 37 °C for 48 h. Then, the cell count was performed in MRS agar, and the kinetic parameters and pH were measured. In addition, the resistance of these bacteria to simulated gastrointestinal conditions was assessed. The AE stimulated bacterial growth by maintaining a cell concentration greater than 10 x 106 for 48 h. The growth rates were 0.635 and 0.656 h-1 for L. plantarum as well as 0.391 and 0.516 h-1 for L. acidophilus in the MRS media containing 1% and 2% (w/v) of AE, respectively. The protective effect of the AE under simulated gastrointestinal conditions for L. plantarum and L. acidophilus was significant at pH 7.0 and 2.0, respectively. Overall, the AE presented prebiotic potential in vitro.

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Section: Effect Of Ae On the Growth Of L Plantarum And L Acidophilusmentioning

confidence: 81%

Section: Effect Of Ae On the Growth Of L Plantarum And L Acidophilusmentioning

confidence: 92%

Potencial prebiótico in vitro del extracto atomizado de Swartzia polyphylla, Maytenus macrocarpa y Jatropha macrantha sobre Lactobacillus plantarum y Lactobacillus acidophilus

HUAPAYA¹,

Zavaleta²,

RAMIREZ³

et al. 2022

Manglar

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“…Despite having similar total quantities of metals in cMRS, B1.3 still contained lower amounts of magnesium, aluminum, and calcium compared to strain B1.1. Presently, aside from a few reports suggesting different metal requirements for lactobacilli (27)(28)(29)(30)(31), the importance of metals for LAB growth and ecological interactions are not well understood. The requirement of L. plantarum for manganese has been specifically investigated (30,32), likely because L. plantarum has a high requirement for Mn 2+ compared to other bacteria and Mn 2+ is known to confer resistance against oxidative (33,34) and heat (35) stress.…”

Section: Discussionmentioning

confidence: 99%

Calcium determines Lactiplantibacillus plantarum intraspecies competitive fitness

Wei

Marco

2022

Preprint

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The importance of individual nutrients for microbial strain robustness and coexistence in habitats containing different members of the same species is not well understood. To address this for Lactiplantibacillus plantarum in food fermentations, we performed comparative genomics and examined the nutritive requirements and competitive fitness for L. plantarum strains B1.1 and B1.3 isolated from a single sample of teff injera fermentation batter. Compared to B1.1 and other L. plantarum strains, B1.3 has a smaller genome, limited biosynthetic capacities, and large mobilome. Despite these differences, B1.3 was equally competitive with B1.1 in a suspension of teff flour. In commercially-sourced, nutrient-replete MRS (cMRS) medium, strain B1.3 reached three-fold higher numbers than B1.1 within two days of passage. Because B1.3 growth and competitive fitness was poor in mMRS, a modified MRS lacking beef extract, we used mMRS to identify nutrients needed for robust B1.3 growth. No improvement was observed when mMRS was supplemented with nucleotides, amino acids, vitamins, or monovalent metals. Remarkably, the addition of divalent metal salts increased the growth rate and cell yields of B1.3 in mMRS. Metal requirements were confirmed by Inductively Coupled Plasma Mass Spectrometry, showing that total B1.3 intracellular metal concentrations were significantly (up to 2.7-fold) reduced compared to B1.1. Supplemental CaCl2 conferred the greatest effect, resulting in equal growth between B1.1 and B1.3 over successive five passages in mMRS. Moreover, calcium supplementation reversed a B1.3 strain-specific stationary phase, flocculation phenotype. These findings show how L. plantarum calcium requirements affect competitive fitness at the strain level.ImportanceEcological theory states that the struggle for existence is stronger between closely related species. Contrary to this assertion, fermented foods frequently sustain conspecific individuals, despite their high levels of phylogenetic relatedness. Therefore, we investigated two isolates of Lactiplantibacillus plantarum B1.1 and B1.3 randomly selected from a single batch of teff injera batter. These strains spanned the known genomic and phenotypic range of the L. plantarum species, and in nutrient-replete, laboratory culture medium, strain B1.3 exhibited poor growth and was outcompeted by the more robust strain B1.1. Despite those differences, B1.1 and B1.3 were equally competitive in teff flour. This result shows how these bacteria have adapted for co-existence in that environment. The capacity for the single macronutrient calcium to restore B1.3 competitive fitness in laboratory culture medium suggests that L. plantarum intraspecies diversity found in food systems is fine-tuned to nutrient requirements at the strain level.

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“…Despite having similar total quantities of metals in cMRS, B1.3 still contained smaller amounts of magnesium, aluminum, and calcium than did strain B1.1. Presently, aside from a few reports suggesting different metal requirements for lactobacilli ( 27 – 31 ), the importance of metals for LAB growth and ecological interactions is not well understood. L. plantarum manganese requirements have been specifically investigated ( 30 , 32 , 33 ), likely because L. plantarum contains elevated levels of Mn 2+ compared to other bacteria and because this element is known to confer resistance against oxidative ( 34 , 35 ) and heat ( 36 ) stress.…”

Section: Discussionmentioning

confidence: 99%

Calcium Determines Lactiplantibacillus plantarum Intraspecies Competitive Fitness

Wei

Marco

2022

Appl Environ Microbiol

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Differential Effects of Transition Metals on Growth and Metal Uptake for Two DistinctLactobacillusSpecies

Cited by 16 publications

References 133 publications

Potencial prebiótico in vitro del extracto atomizado de Swartzia polyphylla, Maytenus macrocarpa y Jatropha macrantha sobre Lactobacillus plantarum y Lactobacillus acidophilus

Potencial prebiótico in vitro del extracto atomizado de Swartzia polyphylla, Maytenus macrocarpa y Jatropha macrantha sobre Lactobacillus plantarum y Lactobacillus acidophilus

Calcium determines Lactiplantibacillus plantarum intraspecies competitive fitness

Calcium Determines Lactiplantibacillus plantarum Intraspecies Competitive Fitness

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