Differential effects of the particle core and organic extract of diesel exhaust particles

Totlandsdal, Annike I.; Herseth, Jan Inge; Bølling, Anette Kocbach; Kubátová, Alena; Braun, Artur; Cochran, Richard E.; Refsnes, Magne; Øvrevik, Johan; Låg, Marit

doi:10.1016/j.toxlet.2011.10.025

Cited by 91 publications

(87 citation statements)

References 33 publications

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“…[11] In brief, native particles (∼30 mg) were suspended in MeOH (∼60 mL), and subsequently sonicated in a water bath (30 min), prior to overnight incubation at 4…”

Section: Collection and Preparation Of Particle Suspensions And Extractsmentioning

confidence: 99%

“…Following the first sonication, two additional extractions were performed on the residual particles to investigate if the first extraction was efficient, i.e., a second sonication (each with 60 mL of MeOH) and then a Soxhlet extraction [11] (with 90 mL of MeOH). The Soxhlet extracts of both samples contained 3-4 times more OC than the second sonication extracts (Table 1).…”

Section: Collection and Preparation Of Particle Suspensions And Extractsmentioning

confidence: 99%

“…[11,23] A broad range of PAHs and PAH-derivatives were analysed, of which the complete list of individual organic species is provided in Figures 2 and 3, and Tables 1S and 2S in Supplementary data. Species for which pure analytical standards were not available were tentatively identified by matching MS spectra (at least 90% matching quality) with those obtained from the NIST 2005 MS Spectral Database.…”

Section: Chemical Characterisation Of the Particle Extractsmentioning

confidence: 99%

“…The extractable fraction, as opposed to the particle core, seemed to be responsible for the particle-induced cell death as well as the expression and release of pro-inflammatory mediators. [11] On a similar token, the extractable organic fraction has been reported to account for the majority of the proinflammatory potential of WSPs. [10] Fractionation of PM extracts, combined with biological experiments, allows for investigation of the influence of specific groups of organic compounds.…”

Section: Introductionmentioning

confidence: 99%

“…[6,7] Several studies have indicated that the organic fraction of combustion PM is responsible for the toxicological effects. [8][9][10][11][12][13][14][15] We recently compared the potency of native DEPs, their corresponding methanol extracts and residual particles to induce expression of multiple pro-inflammatory linked genes in human bronchial epithelial cells. The extractable fraction, as opposed to the particle core, seemed to be responsible for the particle-induced cell death as well as the expression and release of pro-inflammatory mediators.…”

Section: Introductionmentioning

confidence: 99%

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The occurrence of polycyclic aromatic hydrocarbons and their derivatives and the proinflammatory potential of fractionated extracts of diesel exhaust and wood smoke particles

Totlandsdal

Øvrevik

Cochran

et al. 2013

Journal of Environmental Science and Health, Part A

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Exposure to combustion emissions, including diesel engine exhaust and wood smoke particles (DEPs and WSPs), has been associated with inflammatory responses. To investigate the possible role of polycyclic aromatic hydrocarbons (PAHs) and PAH-derivatives, the DEPs and WSPs methanol extracts were fractionated by solid phase extraction (SPE), and the fractions were analyzed for more than ∼120 compounds. The pro-inflammatory effects of the fractionated extracts were characterized by exposure of bronchial epithelial lung cells (BEAS-2B). Both native DEPs and WSPs caused a concentration-dependent increase in IL-6 and IL-8 release and cytotoxicity. This is consistent with the finding of a rather similar total content of PAHs and PAH-derivatives. Yet, the samples differed in specific components, suggesting that different species contribute to the toxicological response in these two types of particles. The majority of the IL-6 release and cytotoxicity was induced upon exposure to the most polar (methanol) SPE fraction of extracts from both samples. In these fractions hydroxy-PAHs, carboxy-PAHs were observed along with nitro-amino-PAHs in DEP. However, the biological effects induced by the polar fractions could not be attributed only to the occurrence of PAH-derivatives. The present findings indicate a need for further characterization of organic extracts, beyond an extensive analysis of commonly suspected PAH and PAH-derivatives.

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“…[11] In brief, native particles (∼30 mg) were suspended in MeOH (∼60 mL), and subsequently sonicated in a water bath (30 min), prior to overnight incubation at 4…”

Section: Collection and Preparation Of Particle Suspensions And Extractsmentioning

confidence: 99%

Section: Collection and Preparation Of Particle Suspensions And Extractsmentioning

confidence: 99%

Section: Chemical Characterisation Of the Particle Extractsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The occurrence of polycyclic aromatic hydrocarbons and their derivatives and the proinflammatory potential of fractionated extracts of diesel exhaust and wood smoke particles

Totlandsdal

Øvrevik

Cochran

et al. 2013

Journal of Environmental Science and Health, Part A

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Spirometry for Chronic Obstructive Pulmonary Disease Due to Inhalation of Smoke from Indoor Fires Used for Cooking and Heating

Shaw¹,

Vaughan²,

Smith³

et al. 2019

Revolutionizing Tropical Medicine

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Emission aktivierter Rußpartikel: die Kehrseite der Medaille moderner Dieselmotoren

2013

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Rußvergiftung: Die erhöhte chemische Aktivität, die durch moderne Dieselmotoren auf den emittierten Ruß übertragen wird, ist ein unerwünschter Nebeneffekt der modifizierten Motorenkonstruktion. Dadurch werden Euro-IV- und Euro-VI-Ruße chemisch und biologisch sehr aktiv und gefährlich. Unter Berücksichtigung dieser Tatsache stellt sich die Frage, ob die induzierte Mikro- und Nanostruktur den Effekt der verringerten Nettoemission an Dieselruß-Feinstaub überkompensiert

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Differential effects of the particle core and organic extract of diesel exhaust particles

Cited by 91 publications

References 33 publications

The occurrence of polycyclic aromatic hydrocarbons and their derivatives and the proinflammatory potential of fractionated extracts of diesel exhaust and wood smoke particles

The occurrence of polycyclic aromatic hydrocarbons and their derivatives and the proinflammatory potential of fractionated extracts of diesel exhaust and wood smoke particles

Spirometry for Chronic Obstructive Pulmonary Disease Due to Inhalation of Smoke from Indoor Fires Used for Cooking and Heating

Emission aktivierter Rußpartikel: die Kehrseite der Medaille moderner Dieselmotoren

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