Differential effects of the MEK inhibitor SL327 on the acquisition and expression of ethanol-elicited conditioned place preference and aversion in mice

Rosas, Michela; Porru, Simona; Longoni, R; Spina, Liliana; Peana, Alessandra Tiziana; Collu, Maria; Acquas, Elio

doi:10.1177/0269881116675514

Cited by 5 publications

(22 citation statements)

References 46 publications

(121 reference statements)

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“…It is possible that a different pattern would have emerged had we used additional higher or lower, ethanol doses. The 2.0 g/kg ethanol dose, however, is a standard dose for inducing CPP in mice and has been used in several influential studies that assessed factors influencing the expression of ethanol’s motivational effects 61 – 64 .…”

Section: Discussionmentioning

confidence: 99%

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

Pautassi

Suarez

Hoffmann

et al. 2017

Sci Rep

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Environmental enrichment (EE) provides a non-pharmacological tool to alter drug-induced reward, yet its effects on ethanol-induced reward remain controversial. We analyzed adolescent vs. adult (mice) differences in the influence of EE on ethanol-induced conditioned place preference (CPP). The effects of these treatments on brain-derived neurotrophic factor (BDNF) levels in the prefrontal cortex were examined in a separate group of animals. Ethanol-induced CPP was found in adults, and it was similar in EE and in animals reared under standard housing conditions (SC). Adolescents kept under EE, but not those in SC, exhibited CPP. Among SC, but not among EE, adolescents, BDNF levels were significantly lower in those treated with ethanol than in those given vehicle. These results indicate that, compared to adults, adolescent exhibited reduced sensitivity to ethanol’s rewarding effects, yet the youth but not the adults exhibited sensitivity to the promoting effect of EE upon CPP by ethanol. Ethanol significantly reduced BDNF levels in adolescents reared under standard housing conditions, but not in adult mice nor in adolescents given EE housing conditions. The present results add to the plethora of adolescent-specific responses to ethanol or to environmental stimuli that may put the youth at risk for escalation of ethanol intake.

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Section: Discussionmentioning

confidence: 99%

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

Pautassi

Suarez

Hoffmann

et al. 2017

Sci Rep

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“…All drugs and vehicle (saline) solutions were administered intraperitoneally. Doses and times were selected based on previous experiments (Acquas et al, 2010; Hilbert et al, 2013; Ibba et al, 2009; López-Cruz et al, 2016; Rosas et al, 2017; SanMiguel et al, 2019; Spina et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…The time spent in one compartment was recorded and taken as an indication of spontaneous preference. Behavioral schedules for backward (CPP) and forward (CPA) conditioning (Figure 1(a) and (b), respectively) were designed based on Rosas et al (2017) and Spina et al (2015), with some modifications related to the timing of pre-treatment with caffeine. In particular, during the second phase (conditioning, days 2–5) of CPP experiments, mice from the different experimental groups were administered caffeine (3 or 15 mg/kg) or saline 20 minutes before ethanol (2 g/kg) or saline, and were returned to their home cage.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, to shed light on the psychopharmacological consequences of the interaction between caffeine and ethanol, the aims of this study were: (a) determining if caffeine pre-treatment, at the doses of 3 and 15 mg/kg, may affect the acquisition of ethanol (2 g/kg)-elicited CPP and CPA (Cunningham et al, 1997; Rosas et al, 2017; Spina et al, 2015) and (b) investigating if caffeine, at these doses, may have the ability to affect ethanol-elicited increases of pERK expression in the brain areas (Ibba et al, 2009) mentioned above. We choose a dose of ethanol (2 g/kg) that in CD-1 mice has demonstrated to reliably elicit robust CPP and CPA (Cunningham et al, 1997; Rosas et al, 2017; Spina et al, 2015). Moreover, the study was also undertaken to verify if the stimuli associated with ethanol during CPP or CPA may induce a differential expression of pERK in the brain areas examined, and if the effects of caffeine on the acquisition of place conditioning may also be reflected in the differential expression of phosphorylated ERK in these brain areas important for associative and motivational processes involved in drug addiction.…”

Section: Introductionmentioning

confidence: 99%

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Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

et al. 2020

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Background: Epidemiological studies indicate a rise in the combined consumption of caffeinated and alcoholic beverages, which can lead to increased risk of alcoholic-beverage overconsumption. However, the effects of the combination of caffeine and ethanol in animal models related to aspects of drug addiction are still underexplored. Aims: To characterize the pharmacological interaction between caffeine and ethanol and establish if caffeine can affect the ability of ethanol (2 g/kg) to elicit conditioned place preference and conditioned place aversion, we administered caffeine (3 or 15 mg/kg) to male CD-1 mice before saline or ethanol. Moreover, we determined if these doses of caffeine could affect ethanol (2 g/kg) elicited extracellular signal-regulated kinase phosphorylation in brain areas, nucleus accumbens, bed nucleus of stria terminalis, central nucleus of the amygdala, and basolateral amygdala, previously associated with this type of associative learning. Results: In the place-conditioning paradigm, caffeine did not have an effect on its own, whereas ethanol elicited significant conditioned-place preference and conditioned-place aversion. Caffeine (15 mg/kg) significantly prevented the acquisition of ethanol-elicited conditioned-place preference and, at both doses, also prevented the acquisition of ethanol-elicited conditioned-place aversion. Moreover, both doses of caffeine also prevented ethanol-elicited extracellular signal-regulated kinase phosphorylation expression in all brain areas examined. Conclusions: The present data indicate a functional antagonistic action of caffeine and ethanol on associative learning and extracellular signal-regulated kinase phosphorylation after an acute interaction. These results could provide exciting grounds for further studies, also in a translational perspective, of their pharmacological interaction modulating other processes involved in drug consumption and addiction.

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“…The texture of the floor was different: in one chamber it was a fine grate, in the other -a smooth dark brown floor. The acquisition of CPP was carried out for 8 days (Rosas et al 2017). On the 1 st day, the rat was placed for 10 minutes in the apparatus with the door open to familiarize and determine the initial preference of one of the apparatus compartments.…”

Section: Conditioned Place Preferencementioning

confidence: 99%

The value of extended amygdala structures in emotive effects of narcogenic with diverse chemical structure

Roik¹,

Lebedev²,

Shabanov³

2019

RRP

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Introduction: Studies on the mechanisms of the reinforcing action of opioid and non-opioid narcotics confirmed the existence in the brain of a specialized system named the extended amygdala. Materials and methods: To clarify the value of the extended amygdala structures (bed nucleus, central nucleus of the amygdala and nucleus accumbens shell) in the mechanisms of unconditioned and conditioned reinforcement activated by various narcogenic, this paper carried out a neuropharmacological analysis of these effects, using blockade of dopamine receptors, GABA, opioids and CRF receptors within these brain structures, as well as an analysis of behavioral responses by self-stimulation (unconditioned reinforcement) and conditioned place preference (CPP) (conditioned reinforcement). Results and discussion: The central amygdala and the bed nucleus have a controlling influence on the hypothalamus, which is predominantly of CRF-, GABA- and dopaminergic nature. Through D1 dopamine receptors,, a direct positive (activating) effect on the lateral hypothalamus is made. The D2 receptor blockade of the nucleus accumbens prevents narcogenic from exerting the reinforcing properties, which are primarily stimulating. The blockade of the D1 receptors of the nucleus accumbens by SCH-23390 prevents the expression of unconditioned and conditioned reinforcing properties of predominantly opiates and opioids. The blockade of GABAA receptors in the nucleus accumbens with bicuculline prevents the manifestation of the primary and secondary reinforcing properties (CPP) of psychostimulant drugs (amphetamine), without affecting the effects of opiates and opioids (fentanyl and leu-enkephalin). Conclusion: The pharmacological analysis proves that CRF, dopamine and GABA receptors are most important for the correction of reinforcement activated by various narcogenic.

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Differential effects of the MEK inhibitor SL327 on the acquisition and expression of ethanol-elicited conditioned place preference and aversion in mice

Cited by 5 publications

References 46 publications

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

Effects of caffeine on ethanol-elicited place preference, place aversion and ERK phosphorylation in CD-1 mice

The value of extended amygdala structures in emotive effects of narcogenic with diverse chemical structure

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