Differential Effects of Scopolamine on Memory Processes in the Object Recognition Test and the Morris Water Maze Test in Mice

Kim, Dong Hyun; Ryu, Jong Hoon

doi:10.4062/biomolther.2008.16.3.173

Cited by 16 publications

(10 citation statements)

References 23 publications

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“…The MWM test was performed to evaluate the escape latency (the time to reach the hidden platform), traveled distance (the length of swim path), and times across platform of mice (at age of about 9 month) as described in previous studies ( 21 , 22 ). Recognition task was preformed as described previously ( 23 ).…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Curcumin Exerts Effects on the Pathophysiology of Alzheimer’s Disease by Regulating PI(3,5)P2 and Transient Receptor Potential Mucolipin-1 Expression

et al. 2017

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BackgroundTo validate our speculation that curcumin may ameliorate Alzheimer’s disease (AD) pathogenesis by regulating PI(3,5)P2 and transient receptor potential mucolipin-1 (TRPML1) expression levels.MethodsWe developed an animal model presenting AD by APP/PS1 transgenes. The mouse clonal hippocampal neuronal cell line HT-22 was treated with amyloid-β1-42 (Aβ1-42). Curcumin was administrated both in vivo and in vitro. MTS assay was used to detect cell viability, and the lysosomal [Ca2+] ion concentration was detected. The number of autophagosomes was detected by the transmission electron microscopic examination. Illumina RNA-seq was used to analyze the different expression patterns between Aβ1-42-treated cells without and with curcumin treatment. The protein level was analyzed by the Western blotting analysis. PI(3,5)P2 or TRPML1 was knocked down in HT-22 cells or in APP/PS1 transgenic mice. Morris water maze and recognition task were performed to trace the cognitive ability.ResultsCurcumin increased cell viability, decreased the number of autophagosomes, and increased lysosomal Ca2+ levels in Aβ1-42-treated HT-22 cells. Sequencing analysis identified TRPLML1 as the most significantly upregulated gene after curcumin treatment. Western blotting results also showed that TRPML1 was upregulated and mTOR/S6K signaling pathway was activated and markers of the autophagy–lysosomal system were downregulated after curcumin use in Aβ1-42-treated HT-22 cells. Knockdown of PI (3,5)P2 or TRPML1 increased the protein levels of markers of the autophagy–lysosomal system after curcumin use in Aβ1-42-treated HT-22 cells, inhibited mTOR/S6K signaling pathway, increased the protein levels of markers of the autophagy–lysosomal system after curcumin use in APP/PS1 mice. Besides, knockdown of PI(3,5)P2 or TRPML1 reversed the protective role of curcumin on memory and recognition impairments in mice with APP/PS1 transgenes.ConclusionTo some extent, it suggested that the effects of curcumin on AD pathogenesis were, at least partially, associated with PI(3,5)P2 and TRPML1 expression levels.

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Section: Methodsmentioning

confidence: 99%

RETRACTED: Curcumin Exerts Effects on the Pathophysiology of Alzheimer’s Disease by Regulating PI(3,5)P2 and Transient Receptor Potential Mucolipin-1 Expression

et al. 2017

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“…The passive avoidance task was carried out as described elsewhere (Kim and Ryu, 2008). At the first training day, for the acquisition trial, a mouse was initially placed in the light compartment and the door between the two compartments was opened 10 s later.…”

Section: Passive Avoidance Taskmentioning

confidence: 99%

GABAA Receptor Blockade Enhances Memory Consolidation by Increasing Hippocampal BDNF Levels

Kim

Park

et al. 2011

Neuropsychopharmacol

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Memory consolidation is the process by which acquired information is converted to something concrete to be retrieved later. Here we examined a potential role for brain-derived neurotrophic factor (BDNF) in mediating the enhanced memory consolidation induced by the GABA(A) receptor antagonist, bicuculline methiodide. With the administration of an acquisition trial in naïve mice using a passive avoidance task, mature BDNF (mBDNF) levels were temporally changed in the hippocampal CA1 region, and the lowest levels were observed 9 h after the acquisition trial. In the passive avoidance task, bicuculline methiodide administration within 1 h of training but not after 3 h significantly increased latency time in the retention trial 24 h after the acquisition trial. Concomitantly, 1 h post-training administration of bicuculline methiodide, which enhanced memory consolidation, significantly increased mBDNF levels 9 h after training compared to those of the vehicle-treated control group. In addition, exogenous human recombinant BDNF (hrBDNF) administration 9 h after training into the hippocampal CA1 region facilitated memory consolidation confirming that the increase in mBDNF at around 9 h after training plays a key role in the enhancement of memory consolidation. Moreover, the increases in latency time and immediate early gene expressions by bicuculline methiodide or hrBDNF were significantly blocked by anisomycin, a protein synthesis inhibitor, K252a, a tyrosine receptor kinase (Trk) inhibitor, or anti-TrkB IgG. These findings suggest that the increase in the level of mBDNF and its function during a restricted time window after training are required for the enhancement of memory consolidation by GABA(A) receptor blockade.

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“…Using the similar groups of animals, we also investigated the ameliorative potential of okra peel on HFD-induced cognitive deficits using the MWM task. The MWM task tests the ability of mice to follow the distal cues to navigate from start positions located around the pool to seek a submerged escape platform [ 48 , 56 ]. Spatial learning is estimated across repeated trials, and reference memory is determined by preference to the platform zone or quadrant when the platform is moved or removed during the probe trial [ 49 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…The MWM task tests the ability of mice to follow the distal cues to navigate from start positions located around the pool to seek a submerged escape platform [ 48 , 56 ]. Spatial learning is estimated across repeated trials, and reference memory is determined by preference to the platform zone or quadrant when the platform is moved or removed during the probe trial [ 49 , 56 ]. Generally, all the groups showed improvement over the four days of acquisition trials, which denotes the animals’ capability in learning to navigate through the maze to locate the platform ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

Prom-in

Kaewsrichan

Wangpradit

et al. 2020

IJERPH

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Okra peel exhibits numerous therapeutic effects. This study explores the potential ameliorative effects of okra peel powder on high-fat-diet (HFD)-induced hypercholesterolemia and cognitive deficits. Thirty-six C57BL/6J male mice were randomly divided into six groups (n = 6 per group): (i) control, mice fed with a normal diet; (ii) HFD, mice fed with HFD; (iii) HFD-SIM, mice fed with HFD and given simvastatin (20 mg/kg/day); (iv) HFD-OP1; (v) HFD-OP2; (vi) HFD-OP3, mice fed with HFD and okra peel (200, 400, or 800 mg/kg/day, respectively). Following 10 weeks of treatments, the mice were subjected to the Morris water maze (MWM). Parameters such as weekly average body weight, food intake, and blood lipid profiles were also recorded. The HFD group showed a profound increase in total cholesterol and low-density lipoprotein concentration compared to the control group. All okra-treated and HFD-SIM groups performed better than the HFD group during acquisition trials, whereas only the HFD-OP1 produced a significantly higher number of entries into the platform zone during the probe trial. In sum, all three okra doses improved the learning ability of the mice. However, only the lowest dose of okra significantly improved the spatial reference memory retention.

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Differential Effects of Scopolamine on Memory Processes in the Object Recognition Test and the Morris Water Maze Test in Mice

Cited by 16 publications

References 23 publications

RETRACTED: Curcumin Exerts Effects on the Pathophysiology of Alzheimer’s Disease by Regulating PI(3,5)P2 and Transient Receptor Potential Mucolipin-1 Expression

RETRACTED: Curcumin Exerts Effects on the Pathophysiology of Alzheimer’s Disease by Regulating PI(3,5)P2 and Transient Receptor Potential Mucolipin-1 Expression

GABAA Receptor Blockade Enhances Memory Consolidation by Increasing Hippocampal BDNF Levels

Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

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