Differential effects of (R)-, (R, S)- and (S)-8-hydroxy-2-(di-n-propylamino)tetralin on hippocampal serotonin release and induction of hypothermia in awake rats

Yoshitake, Takashi; Kehr, Ján

doi:10.1016/j.lfs.2003.10.024

Cited by 9 publications

(6 citation statements)

References 32 publications

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“…Dose calculations were based on (Rodgers and Cole, 1994; Yoshitake and Kehr, 2004). One group of mice received either 8-OH DPAT (1 mg/kg dissolved in saline, 0.1 ml/20 g) or WAY100135 (10 mg/kg dissolved in saline, 0.1 ml/20 g), and the control group received an equivalent volume of saline.…”

Section: Methodsmentioning

confidence: 99%

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

Klempin

Babu

Tonelli

et al. 2010

Front. Mol.Neurosci.

120

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Serotonin (5-HT) appears to play a major role in controlling adult hippocampal neurogenesis and thereby it is relevant for theories linking failing adult neurogenesis to the pathogenesis of major depression and the mechanisms of action of antidepressants. Serotonergic drugs lacked acute effects on adult neurogenesis in many studies, which suggested a surprisingly long latency phase. Here we report that the selective serotonin reuptake inhibitor fluoxetine, which has no acute effect on precursor cell proliferation, causes the well-described increase in net neurogenesis upon prolonged treatment partly by promoting the survival and maturation of new postmitotic neurons. We hypothesized that this result is the cumulative effect of several 5-HT-dependent events in the course of adult neurogenesis. Thus, we used specific agonists and antagonists to 5-HT1a, 2, and 2c receptor subtypes to analyze their impact on different developmental stages. We found that 5-HT exerts acute and opposing effects on proliferation and survival or differentiation of precursor cells by activating the diverse receptor subtypes on different stages within the neuronal lineage in vivo. This was confirmed in vitro by demonstrating that 5-HT1a receptors are involved in self-renewal of precursor cells, whereas 5-HT2 receptors effect both proliferation and promote neuronal differentiation. We propose that under acute conditions 5-HT2 effects counteract the positive proliferative effect of 5-HT1a receptor activation. However, prolonged 5-HT2c receptor activation fosters an increase in late-stage progenitor cells and early postmitotic neurons, leading to a net increase in adult neurogenesis. Our data indicate that serotonin does not show effect latency in the adult dentate gyrus. Rather, the delayed response to serotonergic drugs with respect to endpoints downstream of the immediate receptor activity is largely due to the initially antagonistic and un-balanced action of different 5-HT receptors.

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Section: Methodsmentioning

confidence: 99%

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

Klempin

Babu

Tonelli

et al. 2010

Front. Mol.Neurosci.

120

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“…Atropine (Hentschke et al 2007), DOI hydrochloride (Monti et al 1990), ketanserin tartrate (Kirov and Moyanova 1998), Ro4368554 (Monti and Jantos 2011), and SB-269970 hydrobromide (Westrich et al 2013) were obtained from Sigma-Aldrich (St. Louis, MO). Cimetidine (Monti et al 1986), diphenhydramine hydrochloride (Monti et al 1986), GR-125487 (Galeotti et al 1998), MDL 11,939 (Dudley et al 1988), methysergide maleate (Ishida et al 2007), MK-212 hydrochloride (Hemrick-Luecke and Fuller 1996), nicergoline (Ogawa et al 1993), ondansentron hydrochloride (Ye et al 2001), propranolol hydrochloride (Zarrindast et al 2004, RS-102221 hydrochloride (Bonhaus et al 1998), SB-699551 (Thomas et al 2006), SKF83566 hydrobromide (Meyer et al 1993), (S)-(-)-sulpiride (Dimpfel 2008), TCB-2 , TCS-1102 (Winrow et al 2010), thioperamide (Monti et al 1991), and 8-OH-DPAT hydrobromide (Yoshitake and Kehr 2004) were obtained from Tocris Bioscience (Ellisville, MO). Cimetidine (10 mg/kg ip), diphenhydamine (10 mg/kg ip), DOI (0.3-3 mg/kg ip), GR-125487 (1-30 mg/kg ip), methysergide (0.3-10 mg/kg ip), MK-212 (3-30 mg/kg ip), ondansetron (0.3-10 mg/kg ip), propranolol (10 mg/kg ip), SB-269970 (0.3-10 mg/kg ip), SKF83566 (1 mg/kg ip), TCB-2 (0.3-10 mg/kg ip), and 8-OH-DPAT (0.3-3 mg/kg ip) were dissolved in doubledistilled H 2 O; atropine (10 mg/kg sc), ketanserin (0.1-3 mg/kg ip), MDL 11,939 (0.3-10 mg/kg ip), sulpiride (10 mg/kg ip), thioperamide (4 mg/kg sc), and TCS-1102 (40 mg/kg sc) were dissolved in ethanol; nicergoline (1 mg/kg ip), Ro4368554 (0.3-10 mg/kg ip), RS-102221 (0.3-10 mg/kg ip), and SB-699551 (1-30 mg/kg ip) were dissolved in dimethyl sulfoxide.…”

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confidence: 99%

5-HT_2A receptor activation is necessary for CO₂-induced arousal

Buchanan

Smith

MacAskill

et al. 2015

Journal of Neurophysiology

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Buchanan GF, Smith HR, MacAskill A, Richerson GB. 5-HT 2A receptor activation is necessary for CO 2 -induced arousal. J Neurophysiol 114: 233-243, 2015. First published April 29, 2015 doi:10.1152/jn.00213.2015.-Hypercapnia-induced arousal from sleep is an important protective mechanism pertinent to a number of diseases. Most notably among these are the sudden infant death syndrome, obstructive sleep apnea and sudden unexpected death in epilepsy. Serotonin (5-HT) plays a significant role in hypercapniainduced arousal. The mechanism of 5-HT's role in this protective response is unknown. Here we sought to identify the specific 5-HT receptor subtype(s) involved in this response. Wild-type mice were pretreated with antagonists against 5-HT receptor subtypes, as well as antagonists against adrenergic, cholinergic, histaminergic, dopaminergic, and orexinergic receptors before challenge with inspired CO 2 or hypoxia. Antagonists of 5-HT 2A receptors dose-dependently blocked CO 2 -induced arousal. The 5-HT 2C receptor antagonist, RS-102221, and the 5-HT 1A receptor agonist, 8-OH-DPAT, attenuated but did not completely block CO 2 -induced arousal. Blockade of non-5-HT receptors did not affect CO 2 -induced arousal. None of these drugs had any effect on hypoxia-induced arousal. 5-HT 2 receptor agonists were given to mice in which 5-HT neurons had been genetically eliminated during embryonic life (Lmx1b f/f/p ) and which are known to lack CO 2 -induced arousal. Application of agonists to 5-HT 2A , but not 5-HT 2C , receptors, dose-dependently restored CO 2 -induced arousal in these mice. These data identify the 5-HT 2A receptor as an important mediator of CO 2 -induced arousal and suggest that, while 5-HT neurons can be independently activated to drive CO 2 -induced arousal, in the absence of 5-HT neurons and endogenous 5-HT, 5-HT receptor activation can act in a permissive fashion to facilitate CO 2 -induced arousal via another as yet unidentified chemosensor system.

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“…In a similar manner, we have shown that repeated administration of the prototypical 5-HT 1A receptor agonist 8-hydroxy-2-(di-npropylamino)-tetralin (8-OH-DPAT) reduced basal 5-HT levels in the rat hippocampus to a lesser extent than the first administration, indicating a rapid desensitization or internalization of the 5-HT 1A receptors in the raphe nuclei (Yoshitake et al, 2003c). In a following study (Yoshitake and Kehr, 2004), it was demonstrated that the (R)-and (S)-optical isomers and the (R,S)-racemate of 8-OH-DPAT differentially reduced 5-HT release in the ventral hippocampus of awake rats. Thus, (R)-8-OH-DPAT (0.3 mg/kg s.c.) and the (R,S)-racemate caused a marked reduction of 5-HT levels, to 41 and 46%, respectively, whereas (S)-8-OH-DPAT (0.3 mg/kg s.c.) caused maximal reduction of 5-HT levels only to 74% of controls in both perfusion groups, as shown in Fig.…”

Section: Derivatization Of 5-ht With Bamentioning

confidence: 67%

“…In recent years, we have developed several novel analytical strategies (Ishida et al, 1991Yoshitake et al, 2001Yoshitake et al, , 2003aYoshitake et al, , 2004aFujino et al, 2002Fujino et al, , 2003Kehr et al, 2001Kehr et al, , 2002Yamamguchi et al, 1999;Nohta et al, 1984;Kehr, 1994;Yoshitake and Kehr, 2004) for ultra-sensitive fluorescence detection of monoaminergic neurotransmitters 5-HT and NE in the microdialysates and other biological samples, following their derivatization with benzylamine (BA) and further, for simultaneous determination of 5-HT, NE and DA following two-step derivatization with BA and 1,2-diphenylethylenediamine (DPE). The purpose of the present paper is to provide an overview on optimization of derivatization chemistries for individual monoamines and their simultaneous determination by microbore LC, as well as some typical applications for studying the effects of various behavioral and pharmacological stimuli on extracellular levels of 5-HT, NE and DA in the rat and mouse brains.…”

Section: Introductionmentioning

confidence: 99%

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

Yoshitake

Kehr

Todoroki

et al. 2005

Biomedical Chromatography

102

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The present paper provides an overview on currently developed derivatization chemistries and techniques for determination of monoamine neurotransmitters serotonin (5-HT), norepinephrine (NE) and dopamine (DA) in microdialysis samples by microbore liquid chromatography with fluorescence detection. In mild alkaline conditions, 5-hydroxyindoles and catecholamines react with benzylamine (BA), forming highly fluorescent 2-phenyl-4,5-pyrrolobenzoxazoles and 2-phenyl(4,5-dihydropyrrolo) [2,3-f]benzoxazoles, respectively. However, for derivatization of DA a higher fluorescence intensity was achieved for reaction with 1,2-diphenylethylenediamine (DPE) rather than with BA, therefore for simultaneous determination of 5-HT, NE and DA in brain microdialysates, a two-step derivatization with BA followed by DPE was developed. The detection limits for 5-HT, NE and DA were 0.2, 0.08 and 0.13 fmol, respectively, in an injection volume of 20 microL, which corresponds to concentrations of 30, 12 and 19.5 pm, respectively in standard solution prior to derivatization. The experimental data presented demonstrate the ability of the technique to simultaneously monitor neuronally releasable pools of monoamine neurotransmitters in the rat and mouse brains at basal conditions and following pharmacological treatments or physiological stimuli. These techniques play an important role in drug discovery and clinical investigation of psychiatric and neurological diseases such as depression, schizophrenia and Parkinson's disease.

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Differential effects of (R)-, (R, S)- and (S)-8-hydroxy-2-(di-n-propylamino)tetralin on hippocampal serotonin release and induction of hypothermia in awake rats

Cited by 9 publications

References 32 publications

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

5-HT_2A receptor activation is necessary for CO₂-induced arousal

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

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Differential effects of (R)-, (R, S)- and (S)-8-hydroxy-2-(di-n-propylamino)tetralin on hippocampal serotonin release and induction of hypothermia in awake rats

Cited by 9 publications

References 32 publications

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

Oppositional effects of serotonin receptors 5-HT1a, 2, and 2c in the regulation of adult hippocampal neurogenesis

5-HT2A receptor activation is necessary for CO2-induced arousal

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

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5-HT_2A receptor activation is necessary for CO₂-induced arousal