Differential effects of oligosaccharides on the effectiveness of ampicillin against Escherichia coli in vitro

Asadpoor, Mostafa; Varasteh, Soheil; Pieters, Roland J.; Folkerts, Gert; Braber, Saskia

doi:10.1016/j.phanu.2021.100264

Cited by 8 publications

(6 citation statements)

References 63 publications

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“…In both cases, AOS seem to competitively inhibit the binding of LPS to the cell surface receptors, such as TLR4, subsequently repressing NF-κΒ p65-mediated downstream inflammatory cascades [ 228 ]. These results agree with another study on HT-29 cells, demonstrating that AOS pretreatment attenuates E. coli -induced IL-8 release, establishing this oligosaccharide as a potent anti-inflammatory agent capable of protecting the integrity of intestinal TJs [ 234 ].…”

Section: Non-digestible Oligosaccharides: Their Role In Tj Modulationsupporting

confidence: 91%

Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut

et al. 2022

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The epithelial monolayer is the primary determinant of mucosal barrier function, and tight junction (TJ) complexes seal the paracellular space between the adjacent epithelial cells and represent the main “gate-keepers” of the paracellular route. Impaired TJ functionality results in increased permeation of the “pro-inflammatory” luminal contents to the circulation that induces local and systemic inflammatory and immune responses, ultimately triggering and/or perpetuating (chronic) systemic inflammatory disorders. Increased gut leakiness is associated with intestinal and systemic disease states such as inflammatory bowel disease and neurodegenerative diseases such as Parkinson’s disease. Modulation of TJ dynamics is an appealing strategy aiming at inflammatory conditions associated with compromised intestinal epithelial function. Recently there has been a growing interest in nutraceuticals, particularly in non-digestible oligosaccharides (NDOs). NDOs confer innumerable health benefits via microbiome-shaping and gut microbiota-related immune responses, including enhancement of epithelial barrier integrity. Emerging evidence supports that NDOs also exert health-beneficial effects on microbiota independently via direct interactions with intestinal epithelial and immune cells. Among these valuable features, NDOs promote barrier function by directly regulating TJs via AMPK-, PKC-, MAPK-, and TLR-associated pathways. This review provides a comprehensive overview of the epithelial barrier-protective effects of different NDOs with a special focus on their microbiota-independent modulation of TJs.

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Section: Non-digestible Oligosaccharides: Their Role In Tj Modulationsupporting

confidence: 91%

Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut

et al. 2022

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“…The antibacterial capacity of AOS and COS against GBS V and S. aureus wood 46 was determined via analyzing the minimum inhibitory concentration following the method as described previously ( Asadpoor et al, 2021b ). AOS and COS were serially diluted in 96-well U-bottom polypropylene plates (Corning Costar, Cambridge, MA, United States) to reach 100μl final volume with concentrations ranging from 16 to 0.25%.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

et al. 2021

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The bacterial pathogens Streptococcus agalactiae (GBS) and Staphylococcus aureus (S. aureus) cause serious infections in humans and animals. The emergence of antibiotic-resistant isolates and bacterial biofilm formation entails the urge of novel treatment strategies. Recently, there is a profound scientific interest in the capabilities of non-digestible oligosaccharides as antimicrobial and anti-biofilm agents as well as adjuvants in antibiotic combination therapies. In this study, we investigated the potential of alginate oligosaccharides (AOS) and chitosan oligosaccharides (COS) as alternative for, or in combination with antibiotic treatment. AOS (2–16%) significantly decreased GBS V growth by determining the minimum inhibitory concentration. Both AOS (8 and 16%) and COS (2–16%) were able to prevent biofilm formation by S. aureus wood 46. A checkerboard biofilm formation assay demonstrated a synergistic effect of COS and clindamycin on the S. aureus biofilm formation, while AOS (2 and 4%) were found to sensitize GBS V to trimethoprim. In conclusion, AOS and COS affect the growth of GBS V and S. aureus wood 46 and can function as anti-biofilm agents. The promising effects of AOS and COS in combination with different antibiotics may offer new opportunities to combat antimicrobial resistance.

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“…61 Several studies have illustrated that glycosides can inhibit the growth of Escherichia coli and promote intestinal health. 62,63 Aminoglycosides are one source of antibiotics, which can effectively inhibit MDR bacteria via bactericidal activation based on quorum sensing. In addition, the mutant prevention concentration was proposed to reduce the phenomenon of MDR.…”

Section: Glycosidesmentioning

confidence: 99%

Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking

Yang

Chen

et al. 2022

Phytochemical Analysis

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Introduction: Widespread use of antibiotics has led to an increase in bacterial multiple drug resistance, thereby searching for natural antimicrobial agents from plants becomes an effective and alternative approach. In the present study, we selected six foodborne bacteria to evaluate the antibacterial activities of 12 medicinal plants ethyl acetate (EA) extracts. Objective: This study aims to search for natural antibiotic substitutes from plant extracts. The antibacterial components were further discussed through chemometric and mass spectroscopic analyses. Methodology: Agar well diffusion and the microdilution methods were used to test the antibacterial activity. Total phenolic content (TPC) and total flavonoid content (TFC) were used to judge the active phytochemicals. To further characterise the potential antibacterial components, an ultra-performance liquid chromatographyquadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) coupled with Pearson correlation and feature-based molecular network (FBMN) were proposed. Results: Most of the plant extracts possessed antibacterial activity against Bacillus subtilis and Salmonella typhi. Toona sinensis shoots and Firmiana simplex barks showed high inhibitory activities against Staphylococcus aureus, Shigella dysenteriae, andEscherichia coli strains with minimum inhibitory concentrations (MICs) of 1.56, 0.78, and 0.39 mg/mL, respectively. Salmonella typhi was highly sensitive to Firmiana simplex barks with an inhibitory diameter up to 21.67 ± 0.95 mm, and MIC at 0.78 mg/ mL. Moreover, Toona sinensis shoots and Firmiana simplex barks had the highest TPCs. Conclusion:Our results indicated that Toona sinensis shoots, Koelreuteria paniculate seeds, and Firmiana simplex barks could be supplied as potential sources of antimicrobial agents. Furthermore, 36 potential bioactive compounds were identified mainly as polyphenols, glycosides, and terpenoids.

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Differential effects of oligosaccharides on the effectiveness of ampicillin against Escherichia coli in vitro

Cited by 8 publications

References 63 publications

Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut

Direct Action of Non-Digestible Oligosaccharides against a Leaky Gut

Antimicrobial Activities of Alginate and Chitosan Oligosaccharides Against Staphylococcus aureus and Group B Streptococcus

Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking

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