Differential Effects of Nitrogen Limitation on Photosynthetic Efficiency of Photosystems I and II in Microalgae

Berges, John A.; Charlebois, Denis; Mauzerall, D.; Falkowski, Paul G.

doi:10.1104/pp.110.2.689

Cited by 231 publications

(163 citation statements)

References 22 publications

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“…Previous indirect determinations from F 730 and a chl spectra have shown that cyanobacteria have the lowest proportion of total light absorption by PSII (Johnsen and Sakshaug 1996;Lutz et al 2001). Berges et al (1996) measured the effective absorption and concentration of reaction centers for both PSI and PSII in T. weissfloggii under nutrient replete growth conditions and demonstrated that 46% of total absorption was from photosynthetic pigments associated with PSII. This value is very similar to the value that we report for T. weissflogii.…”

Section: Figmentioning

confidence: 99%

“…Compared to eukaryotic phytoplankton, cyanobacteria have a high n PSII (Barlow and Alberte 1985) and much lower ratios of PSII to PSI (Barlow and Aberte 1985;Berges et al 1996;Falkowski and Raven 1997). In addition, most chlorophyll a is associated with PSI.…”

Section: Hl:d Tertiolecta Ll (ٗ) and P Minimum Hl:e Huxleyi Ll (᭞)mentioning

confidence: 99%

“…As a result, σ PSII may vary in situ by a factor of about 1.5 to 3 across a range of temporal and spatial scales (Greene et al 1994;Vasiliev et al 1994;Behrenfeld et al 1996;Behrenfeld and Kolber 1999;Gorbunov et al 2001). However, few measurements of σ PSII have been obtained from individual taxa under controlled conditions (Dubinsky et al 1986;Berges et al 1996;Koblízek et al 2001;Raateoja and Seppälä 2001), and it is not known to what extent σ PSII is determined by physiological or taxonomic shifts within and across phytoplankton communities.…”

mentioning

confidence: 99%

“…A loss of reaction center functionality may also increase σ PSII (Falkowski and Kolber 1995) in proportion to the fraction of reaction centers lost and the probability of excitation transfer among the remaining functional reaction centers. This may occur, for example, under conditions of nutrient limitation (Greene et al 1994;Berges et al 1996) and photoinhibition (Vasiliev et al 1994;Babin et al 1996;Gorbunov et al 2001). As a result, σ PSII may vary in situ by a factor of about 1.5 to 3 across a range of temporal and spatial scales (Greene et al 1994;Vasiliev et al 1994;Behrenfeld et al 1996;Behrenfeld and Kolber 1999;Gorbunov et al 2001).…”

mentioning

confidence: 99%

“…1 (1) n PSII changes as a result of photoacclimation (Falkowski et al 1981;Dubinsky et al 1986), photoinhibition (Barlow and Alberte 1985) and nutrient limitation (Herzig and Falkowski 1989;Berges et al 1996). Measurements of n PSII by the oxygen flash yield (Falkowski et al 1981) require high concentrations of living phytoplankton, and the technique is largely impractical for natural samples (Falkowski and Kolber 1995).…”

mentioning

confidence: 99%

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Evaluation of biophysical and optical determinations of light absorption by photosystem II in phytoplankton

Suggett

MacIntyre

Geider

2004

Limnology & Ocean Methods

168

251

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Fast repetition rate (FRR) fluorescence can provide highly resolved estimates of light absorption by photosystem II (PSII), a variable that is critical to bio-optical determinations of phytoplankton productivity. We compared estimates of chlorophyll a-specific light absorption by PSII, a chl PSII , using both biophysical (FRR) and optical (chlorophyll a-specific light absorption coefficient, a chl ) techniques on cultures of phytoplankton from diverse taxa. Biophysical determinations of a chl PSII were obtained from the product of the effective light absorption cross-section of PSII (σ PSII ), measured by FRR fluorescence, and the ratio of PSII reaction centers to chlorophyll a (n PSII ), measured by oxygen flash yields. Both parameters were highly variable between individual taxa. In mixtures of algae, the estimates of σ PSII were largely determined by the taxon that dominated fluorescence intensity. Independent optical estimates of a chl PSII were obtained by weighting the light absorption spectra of photosynthetic pigments by the fluorescence excitation spectra. The biophysical and optical estimates of a chl PSII were highly correlated (r 2 = 0.94) with a slope that was not significantly different from 1 and an intercept of 0. Estimates of productivity using biophysical or optical absorption measurements should therefore be comparable when the latter are adjusted to account for the proportion of light provided for photochemistry into both photosystem I and II. Finally, we show how simultaneous measurements of σ PSII and a chl PSII can be used to derive n PSII where flash-yield measurements are impractical, as is almost universally the case in field measurements.

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Section: Figmentioning

confidence: 99%

Section: Hl:d Tertiolecta Ll (ٗ) and P Minimum Hl:e Huxleyi Ll (᭞)mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of biophysical and optical determinations of light absorption by photosystem II in phytoplankton

Suggett

MacIntyre

Geider

2004

Limnology & Ocean Methods

168

251

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In situ monitoring of chlorophyll a fluorescence in Nannochloropsis oceanica cultures to assess photochemical changes and the onset of lipid accumulation during nitrogen deprivation

Carneiro

Zittelli

Cicchi

et al. 2021

Biotech & Bioengineering

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In situ chlorophyll a fluorescence measurements were applied to monitor changes in the photochemical variables of Nannochloropsis oceanica cultures under nitrogendeplete and nitrogen-replete (control) conditions. In addition, growth, lipid, fatty acid, and pigment contents were also followed. In the control culture, growth was promoted along with pigment content, electron transport rate (ETR), and polyunsaturated fatty acids, while total lipid content and fatty acid saturation level diminished. Under nitrogen-deplete conditions, the culture showed a higher deepoxidation state of the xanthophyll cycle pigments. Fast transients revealed a poor processing efficiency for electron transfer beyond Q A , which was in line with the low ETR due to nitrogen depletion. Lipid content and the de-epoxidation state were the first biochemical variables triggered by the change in nutrient status, which coincided with a 20% drop in the in situ effective quantum yield of PSII (ΔF'/F m '), and a raise in the V j measurements. A good correlation was found between the changes in ΔF'/F m ' and lipid content (r = −0.96, p < 0.01). The results confirm the reliability and applicability of in situ fluorescence measurements to monitor lipid induction in N. oceanica.

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Impact of nitrogen availability upon the electron requirement for carbon fixation in Australian coastal phytoplankton communities

Hughes

Varkey

Doblin

et al. 2018

Limnology & Oceanography

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Nitrogen (N) availability affects phytoplankton photosynthetic performance and regulates marine primary production (MPP) across the global coast and oceans. Bio‐optical tools including Fast Repetition Rate fluorometry (FRRf) are particularly well suited to examine MPP variability in coastal regions subjected to dynamic spatio‐temporal fluctuations in nutrient availability. FRRf determines photosynthesis as an electron transport rate through Photosystem II (ETRPSII), requiring knowledge of an additional parameter, the electron requirement for carbon fixation (KC), to retrieve rates of CO2‐fixation. KC strongly depends upon environmental conditions regulating photosynthesis, yet the importance of N‐availability to this parameter has not been examined. Here, we use nutrient bioassays to isolate how N (relative to other macronutrients P, Si) regulates KC of phytoplankton communities from the Australian coast during summer, when N‐availability is often highly variable. KC consistently responded to N‐amendment, exhibiting up to a threefold reduction and hence an apparent increase in the efficiency with which electrons were used to drive C‐fixation. However, the process driving this consistent reduction was dependent upon initial conditions. When diatoms dominated assemblages and N was undetectable (e.g., post bloom), KC decreased predominantly via a physiological adjustment of the existing community to N‐amendment. Conversely, for mixed assemblages, N‐addition achieved a similar reduction in KC through a change in community structure toward diatom domination. We generate new understanding and parameterization of KC that is particularly critical to advance how FRRf can be applied to examine C‐uptake throughout the global ocean where nitrogen availability is highly variable and thus frequently limits primary productivity.

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Differential Effects of Nitrogen Limitation on Photosynthetic Efficiency of Photosystems I and II in Microalgae

Cited by 231 publications

References 22 publications

Evaluation of biophysical and optical determinations of light absorption by photosystem II in phytoplankton

Evaluation of biophysical and optical determinations of light absorption by photosystem II in phytoplankton

In situ monitoring of chlorophyll a fluorescence in Nannochloropsis oceanica cultures to assess photochemical changes and the onset of lipid accumulation during nitrogen deprivation

Impact of nitrogen availability upon the electron requirement for carbon fixation in Australian coastal phytoplankton communities

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