Differential Effects of Methyl Jasmonate on the Expression of the Early Light-Inducible Proteins and Other Light-Regulated Genes in Barley

Abstract

The effects of methyl jasmonate (JA-Me) on early light-inducible protein (ELIP) expression in barley (Hordeum vulgare L. cv Apex) have been studied. Treatment of leaf segments with JA-Me induces the same symptoms as those exhibited by norflurazon bleaching, including a loss of pigments and enhanced light stress that results in increased ELIP expression under both high-and low-light conditions. The expression of both low-and high-molecular-mass ELIP families is considerably down-regulated by JA-Me at the transcript and protein levels. This repression occurs despite increased photoinhibition measurable as a massive degradation of D1 protein and a delayed recovery of photosystem II activity. In JA-Me-treated leaf segments, the decrease of the photochemical efficiency of photosystem II under high light is substantially more pronounced as compared to controls in water. The repression of ELIP expression by JA-Me is superimposed on the effect of the increased light stress that leads to enhanced ELIP expression. The fact that the reduction of ELIP transcript levels is less pronounced than those of light-harvesting complex II and small subunit of Rubisco transcripts indicates that light stress is still affecting gene expression in the presence of JA-Me. The jasmonate-induced protein transcript levels that are induced by JA-Me decline under light stress conditions.

Trienoic fatty acids and plant tolerance of temperature

“…However, we cannot rule out that other biochemical factors associated with the thylakoid membranes are responsible for the death of triple mutant plants grown at high temperature. On this line, linolenic acid is a precursor of jasmonic acid that is involved in many plant responses [29,30].…”

“…However, we cannot rule out that other biochemical factors associated with the thylakoid membranes are responsible for the death of triple mutant plants grown at high temperature. On this line, linolenic acid is a precursor of jasmonic acid that is involved in many plant responses [29,30].The nature of the effects seems also to depend on a specific species that is deficient rather than through the mediation of global changes in membrane fluidity or integrity in the mutant. Approximately one-third of trienoic fatty acids is sufficient to maintain normal photosynthetic function and growth at either lower or higher than normal temperatures.…”

Anatomical and Transcriptomic Studies of the Coleorhiza Reveal the Importance of This Tissue in Regulating Dormancy in Barley

“…However, no relation between ELIPs and dormancy or germination has been reported. ELIPs have also been shown to be induced by heat shock in Arabidopsis (Harari-Steinberg et al, 2001) and to be repressed by jasmonates in barley in a light-independent manner (Wierstra and Kloppstech, 2000). The light-regulated pathway that controls the expression of ELIPs must be different from the pathway that regulates HvNCED1, since HvNCED1 is not regulated by after-ripening.…”

Expression of Early Light Induced Protein in Grapevine and Pea, under Different Conditions and Itis Relation with Photoinhibition

“…(Potter and Kloppstech, 1993;Adamska and Kloppstech, 1994;Hutin et al, 2003;Bruno and Wetzel, 2004). In all cases these proteins were induced by light alone or in combination with other stresses such as high and low temperatures, drought and salinity (Kloppstech et al, 1991;Adamska and Kloppstech, 1994;Zeng et al., 2002) and also by factors such as the stage of plant development, senescence (Binyamin et al, 2001;Norén et al, 2003), light quality (Sävenstrand et al, 2004) or hormonal action (Wiestra and Kloppstech, 2000). In perennial plants ELIPs studies are scarce (Bhalerao et al, 2003;Peng et al, 2008).…”