Differential effects of magnesium on the hydrolysis of ADP and ATP in human term placenta

Martı́nez, Federico; Uribe, Aída; Milán, Rebeca; Espinosa-García, María Teresa; Gracı́a-Pérez, Cecilia; Flores-Herrera, Óscar

doi:10.1016/s1357-2725(02)00021-3

Cited by 5 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Martinez et al (27), this procedure allows the isolation of very purified syncytiotrophoblast mitochondria, as assessed by electron microscopy. Contamination with plasma membrane was found to be Ͻ5% with this method, which did not modify the values of ATP and ADP hydrolysis (28). The method yielded ϳ35 mg of mitochondrial protein from 100 g of wet placental tissue, which agreed with the literature values (12,34,49), and was identical in each clinical group.…”

Section: Isolation Of Mitochondria From Human Placentasupporting

confidence: 82%

Maternal smoking is associated with mitochondrial DNA depletion and respiratory chain complex III deficiency in placenta

Bouhours‐Nouet¹,

May‐Panloup²,

Coutant³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Maternal smoking during pregnancy is often associated with a decrease in placental function, which might lead to intrauterine growth retardation. Because tobacco is known to alter the mitochondrial respiratory function in cardiomyocytes and lung tissue, we hypothesized that placental mitochondrial function could be altered by maternal smoking. Placental mitochondria from 9 smoking and 19 nonsmoking mothers were isolated by differential centrifugation. Mitochondrial oxygen consumption was measured by polarography, and the enzymatic activity of each complex of the electron transport chain was assessed by spectrophotometry. In addition, the relative content in mitochondrial DNA (mtDNA) was determined by real-time quantitative PCR in placentas from seven smoking and seven nonsmoking mothers. We observed a 29% reduction in the enzymatic activity of complex III in the placental mitochondria from smokers compared with nonsmokers (P = 0.03). The relative content of mtDNA (with respect to the beta-globin gene) was reduced by 37% in the placental tissue from smokers compared with nonsmokers (P < 0.02). Both the enzymatic activity of complex III and mtDNA content were inversely related with the daily consumption of cigarettes, and mtDNA content was correlated with cord blood insulin-like growth factor-binding protein-3 (r = 0.74, P < 0.01), a marker of fetal growth. These results show that maternal smoking is associated with placental mitochondrial dysfunction, which might contribute to restricted fetal growth by limiting energy availability in cells.

show abstract

Section: Isolation Of Mitochondria From Human Placentasupporting

confidence: 82%

Maternal smoking is associated with mitochondrial DNA depletion and respiratory chain complex III deficiency in placenta

Bouhours‐Nouet¹,

May‐Panloup²,

Coutant³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Bone development in human beings are requires Ca and if taken in sufficient amount can prevent fractures and bone malformations [20,21]. In the same way, K and Na are required for nerve impulses and ion transport through membranes of tissues; P and Mg are required by the cells for the production of ATP [21,22]. On the contrary, excessive intakes of these minerals can result in toxicity though most minerals have a fairly wide range of adequate intake making deficiency or toxicity relatively rare if proper diets are taken.…”

Section: Icp-msmentioning

confidence: 99%

Organic and Mineral Composition of Seeds of Afrostyrax lepidophyllus Mildbr. and Evaluation of ROS Inhibition and Cytotoxicity of Isolated Compounds

et al. 2019

View full text Add to dashboard Cite

Considering the large consumption of Afrostyrax Lepidophyllus seeds, chemical analyses is necessary to ascertain its use as food spice and also its medicinal potential. Inductively coupled plasma mass spectrometry and gas chromatography mass spectrometry were used to evaluate the mineral and volatile contents respectively while column chromatography enabled the isolation of some compounds. Seventeen compounds were identified by gas chromatography mass spectrometry. Amongst detected sulphur containing compounds, methylsulfinyl(methylthio) methane was in highest amount with percentage composition of 12.25%. Fatty acids and their methyl esters were most abundant constituents notably n-hexadecanoic acid (28.86%) and methyl (E)-9-octadecenoate (21.85%). Some important unsaturated fatty acids notably linoleic acid and oleic acid were identified. Major elements detected in the sample were sodium (42,137.57 ppb), magnesium (456.90 ppb), phosphorus (1703.31 ppb), potassium (4948.88 ppb) and calcium (88.37 ppb. Trace elements detected in the sample are Cr (0.81 ppb), Mn (5.66 ppb), Fe (107.08 ppb), Co (0.14 ppb), Ni (6.77 ppb), Cu (50.05 ppb), Zn (32.06 ppb) and B (6.65 ppb). Other detected elements involved Al (42.25 ppb) and Pb (2.63 ppb) while Cd and Hg were below detectable limits. Five compounds were isolated and identified from their NMR spectral data as tripalmitin (AL23), n-hexadecanoic acid (AL40-45), ursolic acid (AL65), stigmasterol glycoside (AL192) and saccharose (AS329). Ursolic acid showed moderate ROS inhibition with IC 50 = 18.5 ± 3.1 µg/mL compared to 11.2 ± 1.9 µg/mL for ibuprofen. NO inhibition and cytotoxicity on NIH-3T3 cell lines were low for tested compounds. This study fills knowledge gap on chemical analyses of A. lepidophyllus. Keywords ICP-MS • GC-MS • Secondary metabolites • ROS and NO inhibition • Cytotoxicity Abbreviations DCM Dicholoromethane EIMS Electronic impact mass spectrometry HREIMS High resolution electronic impact mass spectrometry MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide GC-FID Gas chromatography flame ionization detector GC-MS Gas chromatography mass spectrometry NO Nitric oxide ROS Reaction oxygen species ICP-MS Inductively coupled plasma mass spectrometry ppb Parts per billion

show abstract

“…Bone development and bone mass in humans are associated with Ca, whose intake in adequate amounts prevents fractures and osteoporosis ( Nordin, 1997 ; Martínez et al, 2002a ; Bass and Chan, 2006 ; Rizzoli et al, 2008 ). Similarly, K and Na are involved in all human tissues for ion transport across membranes; P and Mg are involved in cellular production of adenosine triphosphates (ATP); while S is critical to the biosynthesis of essential amino acids ( Martínez et al, 2002b ; Métayer et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic Dissection of ICP-Detected Nutrient Accumulation in the Whole Seed of Common Bean (Phaseolus vulgaris L.)

Blair

Bhandari

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

Nutrient transport to grain legume seeds is not well studied and can benefit from modern methods of elemental analysis including spectroscopic techniques. Some cations such as potassium (K) and magnesium (Mg) are needed for plant physiological purposes. Meanwhile, some minerals such as copper (Cu), iron (Fe), molybdenum (Mo), and zinc (Zn) are important micronutrients. Phosphorus (P) is rich in legumes, while sulfur (S) concentration is related to essential amino acids. In this research, the goal was to analyze a genetic mapping population of common bean (Phaseolus vulgaris L.) with inductively coupled plasma (ICP) spectrophotometry to determine concentrations of and to discover quantitative trait loci (QTL) for 15 elements in ground flour of whole seeds. The population was grown in randomized complete block design experiments that had been used before to analyze Fe and Zn. A total of 21 QTL were identified for nine additional elements, of which four QTL were found for Cu followed by three each for Mg, Mn, and P. Fewer QTL were found for K, Na and S. Boron (B) and calcium (Ca) had only one QTL each. The utility of the QTL for breeding adaptation to element deficient soils and association with previously discovered nutritional loci are discussed.

show abstract

Differential effects of magnesium on the hydrolysis of ADP and ATP in human term placenta

Cited by 5 publications

References 33 publications

Maternal smoking is associated with mitochondrial DNA depletion and respiratory chain complex III deficiency in placenta

Maternal smoking is associated with mitochondrial DNA depletion and respiratory chain complex III deficiency in placenta

Organic and Mineral Composition of Seeds of Afrostyrax lepidophyllus Mildbr. and Evaluation of ROS Inhibition and Cytotoxicity of Isolated Compounds

Genetic Dissection of ICP-Detected Nutrient Accumulation in the Whole Seed of Common Bean (Phaseolus vulgaris L.)

Contact Info

Product

Resources

About