Differential effects of lysolipids on steroid synthesis in cells expressing endogenous LPA2 receptor

Budnik, Lygia Therese; Brunswig-Spickenheier, Bärbel

doi:10.1194/jlr.m400423-jlr200

Cited by 19 publications

(10 citation statements)

References 54 publications

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“…The high mRNA and protein expressions of LPAR4 compared with the other receptors during the estrous cycle and early pregnancy, as well as the dynamic changes of LPAR2 and LPAR4 during early pregnancy, probably accounts for the contribution of LPA to different events during the estrous cycle and pregnancy, namely the contribution to P4 secretion or modulation of IFNτ action during early pregnancy. The expression of all LPARs in the bovine CL during the estrous cycle and early pregnancy does not completely agree with the results of Budnik and Brunswig-Spickenheier [48], who showed that LPA exerts its actions on bovine luteal cells only via LPAR2. We hypothesize that during the estrous cycle, LPA originating from the CL might exert potential autocrine and paracrine as well as endocrine roles at the time of CL development and maintenance (during early pregnancy), probably acting via all LPARs.…”

Section: Discussionsupporting

confidence: 58%

“…In the present study, we did not find any modulation of the pregnancy recognition signal (IFNτ) action on P4 secretion in the luteal cells of the bovine CL. On the other hand, Budnik and Brunswig-Spickenheier [48] reported that LPA inhibits LH-induced secretion of P4 by bovine CL cells in vitro. In contrast to our cells, the cells used in the study of Budnik and Brunswig-Spickenheier [48] were isolated differently and consisted of the four major kinds of luteal cells, steroidogenic, endothelial, fibroblasts and immune cells.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Budnik and Brunswig-Spickenheier [48] reported that LPA inhibits LH-induced secretion of P4 by bovine CL cells in vitro. In contrast to our cells, the cells used in the study of Budnik and Brunswig-Spickenheier [48] were isolated differently and consisted of the four major kinds of luteal cells, steroidogenic, endothelial, fibroblasts and immune cells. Therefore, the inhibitory effect of LPA on LH-induced secretion of P4 documented by Budnik and Brunswig-Spickenheier [48], might have been mediated by various interactions between these cells.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to our cells, the cells used in the study of Budnik and Brunswig-Spickenheier [48] were isolated differently and consisted of the four major kinds of luteal cells, steroidogenic, endothelial, fibroblasts and immune cells. Therefore, the inhibitory effect of LPA on LH-induced secretion of P4 documented by Budnik and Brunswig-Spickenheier [48], might have been mediated by various interactions between these cells. Moreover, the above authors cultured the cells for 7 days until they were confluent, while we cultured the cells until just after they became attached to the plates.…”

Section: Discussionmentioning

confidence: 99%

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Lysophosphatidic Acid Action in the Bovine Corpus Luteum -An <i>In Vitro</i> Study

Kowalczyk-Zięba

Boruszewska

Saulnier-Blache

et al. 2012

Journal of Reproduction and Development

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Abstract. We examined whether the CL is a site for lysophosphatidic acid (LPA) synthesis and/or a target for LPA action in the bovine reproductive tract. LPA concentrations in the CL tissue increased towards the end of the cycle and were stable during early pregnancy. No changes in the expression of LPA receptors (LPARs) occurred during the estrous cycle. The expressions of LPAR2 and LPAR4 on days 17-19 of pregnancy were higher than those on the respective days of the estrous cycle and higher than those on days 8-10 of pregnancy. LPA stimulated P4 synthesis via 3βHSD stimulation but did not modulate the interferon-tau (IFNτ) influence on P4 synthesis in steroidogenic cells. Moreover, we found LPA-dependent stimulation of IFNτ action on 2,5'-oligoadenylate synthase (OAS1) and ubiquitin-like IFN-stimulated gene 15-kDa protein (ISG15) expression. The present study demonstrated that the CL might be a site of LPA synthesis and target of LPA action in the bovine reproductive tract. We postulate that during the estrous cycle and early pregnancy, LPA exerts autocrine and paracrine effects on the CL mainly via LPAR2 and LPAR4. The stimulatory effect of LPA on P4 synthesis via 3βHSD stimulation and LPA-dependent stimulation of IFNτ action on OAS1 and ISG15 expression suggest that LPA is an additional auxiliary luteosupportive factor in steroidogenic cells. T he corpus luteum (CL) is an endocrine gland that is temporarily formed in the ovary and undergoes regression at the end of the estrous cycle [1]. After ovulation, it forms from the Graafian follicle, grows and vascularizes rapidly. The bovine CL consists of a variety of cell types including large and small luteal cells, endothelial cells, fibroblasts and immune cells [2,3]. In nonpregnant cows, the CL undergoes luteolysis and becomes nonfunctional around days 17-18 after ovulation [4,5]. The main function of the CL both during the cycle and pregnancy is synthesis of progesterone (P4), which plays major roles in the regulation of the length of the estrous cycle and in the implantation of the blastocyst after fertilization [6]. During maternal recognition of pregnancy, the conceptus synthesizes and secretes interferon tau (IFNτ), which protects the CL and extends the estrous cycle [7]. In addition to its antiluteolytic effects, IFNτ increases expression of several IFN-stimulated genes (ISG), such as 2',5'-oligoadenylate synthetase (OAS1) and ubiquitin-like IFNstimulated gene 15-kDa protein (ISG15) in the uterus [8], mammary gland and CL [9] in cattle. However, during maternal recognition of pregnancy, P4 is the main factor responsible for its successful establishment. Luteinizing hormone (LH) is the most important regulator of P4 synthesis [10,11]. The growth and development of the early CL is supported by many factors, including LH, PGs (PGE 2 and PGI 2 ), oxytocin, noradrenaline and growth factors [12][13][14][15]. The CL can also autoregulate the synthesis of P4 [16].Lysophosphatidic acid (LPA) has been shown to affect the reproductive processes in rats [17], pigs [1...

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lysophosphatidic Acid Action in the Bovine Corpus Luteum -An <i>In Vitro</i> Study

Kowalczyk-Zięba

Boruszewska

Saulnier-Blache

et al. 2012

Journal of Reproduction and Development

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“…However, the production of progesterone remained unaltered in hCGs and HGL5 cells exposed to S1P, indicating that this lysosphingolipid is irrelevant for FF-HDLinduced steroid synthesis. In this context, it is notable that S1P was previously shown to suppress progesterone synthesis in late-stage luteal cells and Leydig tumor cells stimulated with luteotropic hormone or with cAMP analogue [42]. Therefore, it cannot be excluded that FF-HDL exerts dual effects on ovarian steroidogenesis that are stimulatory by substrate provision and suppressive by S1P signaling.…”

Section: Discussionmentioning

confidence: 94%

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Becker¹,

Otte²,

Robenek³

et al. 2011

Biology of Reproduction

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Coordinated migration and progesterone production by granulosa cells is critical to the development of the corpus luteum, but the underlying mechanisms remain obscure. Sphingosine 1-phosphate (S1P), which is associated with follicular fluid high-density lipoprotein (FF-HDL), was previously shown to regulate ovarian angiogenesis. We herein examined the effects of S1P and FF-HDL on the function of granulosa lutein cells. Both FF-HDL and S1P induced migration of primary human granulosa lutein cells (hGCs) and the granulosa lutein cell line HGL5. In addition, FF-HDL but not S1P promoted progesterone synthesis, and neither of the two compounds stimulated proliferation of granulosa lutein cells. Polymerase chain reaction and Western blot experiments demonstrated the expression of S1P receptor type 1 (S1PR1), S1PR2, S1PR3, and S1PR5 but not S1PR4 in hGCs and HGL5 cells. The FF-HDL- and S1P-induced granulosa lutein cell migration was emulated by FTY720, an agonist of S1PR1, S1PR3, S1PR4, and S1PR5, and by VPC24191, an agonist of S1PR1 and S1PR3, but not by SEW2871 and phytosphingosine 1-phosphate, agonists of S1PR1 and S1PR4, respectively. In addition, blockade of S1PR3 with CAY1044, suramine, or pertussis toxin inhibited hGC and HGL5 cell migration toward FF-HDL or S1P, while blockade of S1PR1 and S1PR2 with W146 and JTE013, respectively, had no effect. Both FF-HDL and S1P triggered activation of small G-protein RAC1 and actin polymerization in granulosa cells, and RAC1 inhibition with Clostridium difficile toxin B or NSC23766 abolished FF-HDL- and S1P-induced migration. The FF-HDL-associated S1P promotes granulosa lutein cell migration via S1PR3 and RAC1 activation. This may represent a novel mechanism contributing to the development of the corpus luteum.

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Lysophospholipid Signaling in Female and Male Reproductive Systems

2013

Lysophospholipid Receptors

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Differential effects of lysolipids on steroid synthesis in cells expressing endogenous LPA2 receptor

Cited by 19 publications

References 54 publications

Lysophosphatidic Acid Action in the Bovine Corpus Luteum -An <i>In Vitro</i> Study

Lysophosphatidic Acid Action in the Bovine Corpus Luteum -An <i>In Vitro</i> Study

Follicular Fluid High-Density Lipoprotein-Associated Sphingosine 1-Phosphate (S1P) Promotes Human Granulosa Lutein Cell Migration via S1P Receptor Type 3 and Small G-Protein RAC11

Lysophospholipid Signaling in Female and Male Reproductive Systems

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