Differential effects of low and high temperature stress on pollen germination and tube length of hazelnut (Corylus avellana L.) genotypes

Çetinbaş-Genç, Aslıhan; Cai, Giampiero; Vardar, Filiz; Ünal, Meral

doi:10.1016/j.scienta.2019.05.024

Cited by 24 publications

(21 citation statements)

References 62 publications

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“…Based on the results, the germination was inhibited by temperature increment. Consistently, several researchers have been reported the regression of pollen germination by heat stress in many species such as olive, tobacco and hazelnut (Koubouris et al 2009;Parrotta et al 2016;Çetinbaş-Genç et al 2019). The observations also revealed that pollen tube elongation was stimulated at 30 °C when compared to the control.…”

Section: Discussionsupporting

confidence: 80%

“…It has been noticed tumescence on tea pollen tube apex exposed to cold stress (Wang et al 2016). Also, pollen tubes that exposed the low and high temperatures have exhibited swollen tips in hazelnut (Çetinbaş-Genç et al 2019). Moreover, Büyükkartal (2002) has been stated that abnormalities such as bifurcation at tube tips arrested the pollen tube elongation in Trifolium pratense.…”

Section: Discussionmentioning

confidence: 99%

“…Pollen grains are useful indicators for the detection of temperature stress effects because pollen grains have speciesspecific responses to various stress conditions (Mesihovic et al 2016). To detect the response to stress, pollen germination rate, tube length, tube abnormality rate, tube wall or cytoskeleton components are generally used parameters (Jia et al 2017;Çetinbaş-Genç et al 2019). Since callose is the most plentiful ingredient of the pollen tube wall, the researchers generally have focused on the changes in callose accumulation on the tube to demonstrate the effect of heat stress (Parrotta et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae)

Çetinbaş-Genç

2020

Braz. J. Bot

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In this study, the effects of heat stress (30 °C, 35 °C, 40 °C) on pollen performance of Echinopsis chamaecereus Friedrich & Glaetzle were analyzed. Heat-treated pollen grains showed a insignificant reduction in germination rate. Pollen tubes treated by temperatures above 30 °C exhibited a decrease in tube length and a negative effect on pollen performance according to cumulative stress response index data. Pollen tube abnormality was enhanced by increased temperature, and bifurcated pollen tubes were very dominant than the other abnormalities. Callose accumulation on tube tips was enhanced by increased temperature. In bifurcated pollen tubes, one of the tubes blocked by callose plugs or dense callose accumulation was observed on tips. Actin organization showed disruption, and anisotropy of actin filaments increased at temperatures above 30 °C. The findings show that high temperatures cause destructive effects on male reproductive biology even in species such as cacti known to be temperature tolerant.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae)

Çetinbaş-Genç

2020

Braz. J. Bot

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“…To deepen the knowledge about pollination dynamics and improve crop yield, basic and applied research was recently carried out. In particular, the effect of temperature on hazelnut pollen storage (Ellena et al, 2014;Novara et al, 2017), the influence of spermidine and temperature on pollen performance (Çetinbaş-Genç et al, 2019(Çetinbaş-Genç et al, , 2020, and the efficacy of artificial pollination in constrained growth conditions (Ascari et al, 2018) were investigated.…”

Section: Introductionmentioning

confidence: 99%

Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry

et al. 2020

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Impedance flow cytometry (IFC) is a versatile lab-on-chip technology which enables fast and label-free analysis of pollen grains in various plant species, promising new research possibilities in agriculture and plant breeding. Hazelnut is a monoecious, anemophilous species, exhibiting sporophytic self-incompatibility. Its pollen is dispersed by wind in midwinter when temperatures are still low and relative humidity is usually high. Previous research found that hazelnut can be characterized by high degrees of pollen sterility following a reciprocal chromosome translocation occurring in some cultivated genotypes. In this study, IFC was used for the first time to characterize hazelnut pollen biology. IFC was validated via dye exclusion in microscopy and employed to (i) follow pollen hydration over time to define the best pre-hydration treatment for pollen viability evaluation; (ii) test hazelnut pollen viability and sterility on 33 cultivars grown in a collection field located in central Italy, and two wild hazelnuts. The accessions were also characterized by their amount and distribution of catkins in the tree canopy. Pollen sterility rate greatly varied among hazelnut accessions, with one main group of highly sterile cultivars and a second group, comprising wild genotypes and the remaining cultivars, producing good quality pollen. The results support the hypothesis of recurring reciprocal translocation events in Corylus avellana cultivars, leading to the observed gametic semi-sterility. The measured hazelnut pollen viability was also strongly influenced by pollen hydration (Radj2 = 0.83, P ≤ 0.0001) and reached its maximum at around 6 h of pre-hydration in humid chambers. Viable and dead pollen were best discriminated at around the same time of pollen pre-hydration, suggesting that high humidity levels are required for hazelnut pollen to maintain its functionality. Altogether, our results detail the value of impedance flow cytometry for high throughput phenotyping of hazelnut pollen. Further research is required to clarify the causes of pollen sterility in hazelnut, to confirm the role of reciprocal chromosome translocations and to investigate its effects on plant productivity.

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“…Only then, the fertilization takes place. Therefore, this delayed fertilization is between the principal factors threatening hazelnut yield ( Heslop-Harrison et al, 1986 ; Çetinbaş-Genç et al, 2019 ). Pollination and fertilization processes are extremely sensitive to air temperature and relative humidity (RH) ( Kelley, 1979 ; Huo et al, 2014 ; Çetinbaş-Genç et al, 2019 ); for these reasons, specific attention should be given to protect the trees from abnormal and extreme climatic conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of Photo-Selective Shade Nets on Pollination Process and Nut Development of Corylus avellana L.

Guastella¹,

Sigwebela²,

Suarez³

et al. 2020

Front. Plant Sci.

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Hazelnut (Corylus avellana L.) is one of the most appreciated nut crops, which is motivating the cultivation outside its historical production areas. Despite that, there is still limited knowledge about the floral biology of the species and its developmental fruiting stages under different environments. Adverse climatic conditions can threaten the pollination process and fruit development. In South Africa, the deciduous fruit industry identified the net shading as a tool to mitigate the effects of unfavorable abiotic events. The objective of this work was to investigate the effects of photo-selective nets on the pollination process and nut development of C. avellana. Mature hazelnut trees were maintained under netting and compared with the ones in open field. Microscopic examination of female flower and developing nuts were conducted in order to observe the pollen tube growth and the pattern of disodium fluorescein transport into the funiculus and ovule. The results showed differences in pollen tubes growth and timing between the treatments. Generally, trees under nets showed higher rate in pollen tubes developing and reaching the base of the style. On the contrary, the tests carried out in open field showed a higher ratio of pollen tubes arrested in the style. The results also indicated differences in ovules abortion. Developing fruits that showed an interruption point at the funicle level or at junction point of the ovule were classified as aborting fruits (blank nuts at harvest time). A higher rate of abortion was detected in open field compared to the plants under netting. In conclusion, the shade nets influenced the pollen tube growth and the nut development, principally due to micro-climate modification. Therefore, further investigations are needed to analyze the influence of light spectra and to determine the sustainability of photo-selective nets over several years.

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Differential effects of low and high temperature stress on pollen germination and tube length of hazelnut (Corylus avellana L.) genotypes

Cited by 24 publications

References 62 publications

Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae)

Adverse effects of heat stress in relation to actin cytoskeleton on pollen performance of Echinopsis chamaecereus (Cactaceae)

Hazelnut Pollen Phenotyping Using Label-Free Impedance Flow Cytometry

Effect of Photo-Selective Shade Nets on Pollination Process and Nut Development of Corylus avellana L.

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