Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum perforatum

Briskin, Donald P.; Gawienowski, Margaret

doi:10.1016/s0981-9428(01)01326-2

Cited by 106 publications

(62 citation statements)

References 30 publications

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“…In addition, as suggested above, AMF can reduce the N content of the plant (Saia et al 2014a) or readdress amino acid metabolism to the biosynthesis of secondary compounds (Battini et al 2016;Srivastava et al 2016). Other authors (Briskin et al 2000;Briskin and Gawienowski 2001) also showed that a reduction in N availability increased hypericin content without resulting in nitrogen deficiency symptoms. And indeed, we Fig.…”

Section: Discussionmentioning

confidence: 96%

Arbuscular mycorrhizal fungi altered the hypericin, pseudohypericin, and hyperforin content in flowers of Hypericum perforatum grown under contrasting P availability in a highly organic substrate

et al. 2016

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St. John's Wort (Hypericum perforatum) is a perennial herb able to produce water-soluble active ingredients (a.i.), mostly in flowers, with a wide range of medicinal and biotechnological uses. However, information about the ability of arbuscular mycorrhizal fungi (AMF) to affect its biomass accumulation, flower production, and concentration of a.i. under contrasting nutrient availability is still scarce. In the present experiment, we evaluated the role of AMF on growth, flower production, and concentration of bioactive secondary metabolites (hypericin, pseudohypericin, and hyperforin) of H. perforatum under contrasting P availability. AMF stimulated the production of aboveground biomass under low P conditions and increased the production of root biomass. AMF almost halved the number of flowers per plant by means of a reduction of the number of flower-bearing stems per plant under high P availability and through a lower number of flowers per stem in the low-P treatment. Flower hyperforin concentration was 17.5% lower in mycorrhizal than in non-mycorrhizal plants. On the contrary, pseudohypericin and hypericin concentrations increased by 166.8 and 279.2%, respectively, with AMF under low P availability, whereas no effect of AMF was found under high P availability. These results have implications for modulating the secondary metabolite production of H. perforatum. However, further studies are needed to evaluate the competition for photosynthates between AMF and flowers at different nutrient availabilities for both plant and AM fungus.

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Section: Discussionmentioning

confidence: 96%

Arbuscular mycorrhizal fungi altered the hypericin, pseudohypericin, and hyperforin content in flowers of Hypericum perforatum grown under contrasting P availability in a highly organic substrate

et al. 2016

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“…St. John's wort leaves contain two types of glands: translucent glands distributed across the leaf lamina and dark glands located mainly along the leaf margins. The accumulation of hypericins is associated with the dark glands (Briskin and Gawienowski 2001;Zobayed et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

et al. 2008

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Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones.

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“…However, optimization of controlled environments for medicinal plant production is complex. Increasing light levels were shown to increase phytochemical yield in cultivated St. John's wort plants (Briskin and Gawienowski 2001), but higher light intensities did not significantly improve compound recovery when the plants were grown in aseptic bioreactor cultures. Similarly, enriching the culture environment with elevated levels of CO 2 did not significantly improve phytochemical yield .…”

Section: Controlled Environmentmentioning

confidence: 88%

“…Additionally, the hypericin content of St. John's wort tissues is highest when the flower is in full bloom, and levels decrease during the over-blooming period (Seidler-Lożykowska 2003). Changing environments can alter the phytochemical profile of St. John's wort tissues Briskin and Gawienowski 2001) as can the time of harvest (Seidler-Lożykowska 2003). Other environmental factors altering the phytochemical profile include insect herbivory, which can change hypericin and hyperforin contents of the tissues by as much as 30-100% (Sirvent et al 2003).…”

Section: St John's Wort: Chemical Profiles In Thementioning

confidence: 99%

St. John’s wort (Hypericum perforatum L.): Challenges and strategies for production of chemically-consistent plants

Murch¹,

Saxena²

2006

Can. J. Plant Sci.

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Murch, S. J. and Saxena, P. K. 2006. St. John's wort (Hypericum perforatum L.): Challenges and strategies for production of chemically consistent plants. . Plants are by far the most important source of natural therapeutics, and the role of plants in enhancing the longevity and the quality of life is increasingly accepted throughout the world. A series of problems with medicinal plant products, such as contamination with biological and environmental pollutants, quantitative and qualitative variations of bioactive compounds, adulteration with misidentified species, and the concern of unsustainable harvest, has prompted the introduction of regulations to ensure the quality and safety of medicinal plant products in Canada. In the future, Natural Health Products in Canada will be manufactured to a new standard of quality and these changes in the industry have necessitated new approaches to the breeding, production, and processing of medicinal plant tissues. The continuing growth in the medicinal plant marketplace has also brought about the challenge of maintaining a balance between consumer demand and the need to protect medicinal biodiversity. St. John's wort (Hypericum perforatum L.) is one of the most popular medicinal plants with a history of use spanning more than two millennia and modern studies demonstrating efficacy. However, inconsistencies in the results of various clinical trials and difficulties in identifying a specific medicinal molecule with defined pharmaceutical function prompted our efforts to improve St. John's wort products. Development of elite varieties with predictable phytochemical profiles, mass clonal propagation in vitro, large-scale production in sterile environments and controlled environment production systems, have been combined to produce a new standard in the production of St. John's wort. . Les plantes restent de loin la principale source de substances médicinales naturelles et, partout dans le monde, on reconnaît de plus en plus leur rôle dans un accroissement de la longévité et l'amélioration de la qualité de vie. Diverses difficultés (contamination des substances médicinales végé-tales par des polluants organiques et environnementaux, variation de la quantité et de la qualité des composés bioactifs, adultération avec des espèces non identifiées, récolte à des taux insoutenables) ont incité les administrations publiques du Canada à réglementer ces plantes afin d'en garantir la qualité et l'innocuité. Dorénavant, au Canada, les produits de santé naturels seront préparés en fonction d'une nouvelle norme qualitative et ce changement a contraint l'industrie à adopter de nouvelles approches à l'hybridation, à la culture et à la transformation des plantes médicinales. Parallèlement, le marché poursuit son essor, si bien qu'il est difficile de répondre à la demande tout en préservant la biodiversité des plantes. Le millepertuis (Hypericum perforatum L.) figure parmi les plus populaires. On utilise cette plante depuis plus de deux millénaires et des études contemporaines en ont prouvé l'e...

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Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum perforatum

Cited by 106 publications

References 30 publications

Arbuscular mycorrhizal fungi altered the hypericin, pseudohypericin, and hyperforin content in flowers of Hypericum perforatum grown under contrasting P availability in a highly organic substrate

Arbuscular mycorrhizal fungi altered the hypericin, pseudohypericin, and hyperforin content in flowers of Hypericum perforatum grown under contrasting P availability in a highly organic substrate

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

St. John’s wort (Hypericum perforatum L.): Challenges and strategies for production of chemically-consistent plants

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