Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs

Davis, Teresa A.; Fiorotto, Marta L.; Beckett, Philip R.; Burrin, Douglas G.; Reeds, Peter J.; Wray‐Cahen, Diane; Nguyen, Hanh V.

doi:10.1152/ajpendo.2001.280.5.e770

Cited by 79 publications

(121 citation statements)

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“…The PTP1B association with IR in muscle, but not liver, was lower in 7-compared with 26-day-old pigs (* P Ͻ 0.05). high insulin sensitivity of protein synthesis in skeletal muscle of 7-compared with 26-day-old pigs (14,37,46). Importantly, no developmental change in PTP1B activation in liver was detected, which is consistent with the previously reported lack of developmental change in insulin sensitivity in the liver (14,37).…”

Section: Discussionsupporting

confidence: 91%

“…high insulin sensitivity of protein synthesis in skeletal muscle of 7-compared with 26-day-old pigs (14,37,46). Importantly, no developmental change in PTP1B activation in liver was detected, which is consistent with the previously reported lack of developmental change in insulin sensitivity in the liver (14,37). There were no differences in PTP1B activity between fasted and fed pigs in the current study, although studies in intact cells have shown that insulin increases PTP1B activity (25,35).…”

Section: Discussionsupporting

confidence: 90%

“…Furthermore, we also studied the mechanisms involved in PTP1B activation by measuring the association of PTP1B with the IR, the tyrosine phosphorylation of PTP1B, and the association of PTP1B with Grb2 adaptor protein. For comparison, similar measurements were also made in liver, an organ in which insulin does not stimulate protein synthesis (14,15) and which shows no developmental decline in insulin signaling (37).…”

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Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs

Suryawan

Davis

2003

American Journal of Physiology-Endocrinology and Metabolism

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Suryawan, Agus, and Teresa A. Davis. Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs. Am J Physiol Endocrinol Metab 284: E47-E54, 2003. First published September 11, 2002 10.1152/ajpendo.00210.2002The high activity of the insulin-signaling pathway contributes to the enhanced feeding-induced stimulation of translation initiation in skeletal muscle of neonatal pigs. Protein-tyrosine-phosphatase 1B (PTP1B) is a negative regulator of the tyrosine phosphorylation of the insulin receptor (IR) and insulin receptor substrate 1 (IRS-1). The activity of PTP1B is determined mainly by its association with IR and Grb2. We examined the level of PTP1B activity, PTP1B protein abundance, PTP1B tyrosine phosphorylation, and the association of PTP1B with IR and Grb2 in skeletal muscle and liver of fasted and fed 7-and 26-day-old pigs. PTP1B activity in skeletal muscle was lower (P Ͻ 0.05) in 7-compared with 26-day-old pigs but in liver was similar in the two age groups. PTP1B abundances were similar in muscle but lower (P Ͻ 0.05) in liver of 7-compared with 26-day-old pigs. PTP1B tyrosine phosphorylation in muscle was lower (P Ͻ 0.05) in 7-than in 26-day-old pigs. The associations of PTP1B with IR and with Grb2 were lower (P Ͻ 0.05) at 7 than at 26 days of age in muscle, but there were no age effects in liver. Finally, in both age groups, fasting did not have any effect on these parameters. These results indicate that basal PTP1B activation is developmentally regulated in skeletal muscle of neonatal pigs, consistent with the developmental changes in the activation of the insulin-signaling pathway reported previously. Reduced PTP1B activation in neonatal muscle likely contributes to the enhanced insulin sensitivity of skeletal muscle in neonatal pigs.neonate; insulin signaling; insulin sensitivity; protein synthesis THE ENHANCED ACTIVATION of the insulin-signaling pathway leading to translation initiation in skeletal muscle of the neonate after food consumption has an important role in the enhanced responsiveness of muscle protein synthesis to feeding in the neonate (13,16,26,27,37). We have shown that the feeding-induced activation of the insulin receptor (IR), insulin receptor substrate-1 (IRS-1), and phosphatidylinositol 3-kinase (PI 3-kinase), as well as the abundance of the IR, is enhanced in skeletal muscle of the neonatal pig and decreases markedly with development. This developmental decline in the feeding-induced activation of early insulin-signaling components parallels the developmental decline in the feeding-induced activation of downstream signaling proteins, leading to the stimulation of protein synthesis in skeletal muscle and the developmental decrease in the ability of insulin to stimulate muscle protein synthesis. However, the molecular mechanism that regulates the developmental decline in the insulin sensitivity of skeletal muscle in neonatal pigs has not been elucidated.When insulin binds to its receptor, it induces autophosphorylation of the receptor on its ty...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

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Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs

Suryawan

Davis

2003

American Journal of Physiology-Endocrinology and Metabolism

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“…Furthermore, there seems to be a compartmentalization where proteins for export are synthesized on the rough endoplasmic reticulum while constitutive proteins are synthesized on polysomes within the cell cytosol (Connell et al, 1997). However, insulin was not involved in the stimulation of protein synthesis in the liver of neonatal pigs (Davis et al, 2001). Furthermore, no difference in albumin synthesis was found in humans with increased plasma insulin (Ballmer et al, 1995) compared with controls.…”

Section: I/g Ratiomentioning

confidence: 99%

Metabolic differences in hepatocytes of obese and lean pigs

González-Valero

Rodríguez-López

Lachica

et al. 2014

Animal

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There are important differences in terms of metabolic activity, energy utilization and capacity of protein and fat deposition when Iberian and modern pigs are compared. Primary culture of hepatocytes was used to evaluate hepatic function and sensitivity to hormones between breeds without the interference of circulating blood factors. Hepatocytes were isolated from pure Iberian (n = 10) and Landrace (n = 8) pigs of similar BW (24.5 ± 12.1 and 32.9 ± 6.1 kg BW, respectively), by collagenase perfusion. Monolayers were established in medium containing fetal bovine serum for 1 day and switched to serum-free medium for the remainder of the culture period. Hepatocytes were maintained in William's E supplemented with β-mercaptoethanol (0.1 mM), glutamine (2 mM), antibiotics (gentamicin, penicillin, streptomycin and amphotericin B), dimethyl sulfoxide (1 µg/ml), dexamethasone (10 −8 M), insulin (0.173 and 17.3 nM) and glucagon (0.287, 2.87 and 28.7 nM) for 24 to 48 h. Gluconeogenesis (GNG), glycogen degradation, triglycerides (TG) content and esterification, β-hydroxybutyrate (BHB) synthesis, IGF-1 synthesis, albumin and urea synthesis were determined. Iberian pigs had greater capacity of GNG than Landrace (24%, P < 0.05), although no difference in glycogen degradation was found (P > 0.10). TG content and esterification tended to be lower in hepatocytes from Iberian compared with Landrace pigs (12% and 31%, respectively; 0.10 < P < 0.05). Furthermore, addition of free fatty acids (CLA or linoleic acid, 0.2 mM) increased TG content (64%, P < 0.001) although no difference between fatty acids was found. When free fatty acids were compared, a trend toward increased esterification (41%, P = 0.078) was found for CLA. Although glucagon stimulated and insulin inhibited BHB synthesis, no difference between breeds was found (P > 0.10). IGF-1 synthesis was diminished in hepatocytes from Iberian compared with Landrace pigs (16%, P < 0.05). On the contrary, rate of albumin synthesis was greater in Iberian compared with Landrace pigs (58%, P < 0.05). Finally, the capacity of urea synthesis was lower in hepatocytes of Iberian compared with Landrace pigs (37%, P < 0.05). When ammonia was added to the media, urea concentration increased (648%, 1108% and 2791% when 0 mM was compared with 2.5, 5 and 10 mM, respectively). Urea synthesis increased on increasing ammonia content (55% and 325% when 0 mM was compared with 5 and 10 mM, respectively; P < 0.0001). In conclusion, the genetic background accounts for important differences in protein and energy metabolism pathways found in primary culture of hepatocytes from lean and obese pigs.Keywords: hepatocytes, metabolism, pig ImplicationsSeveral studies have shown the existence of numerous dissimilarities in metabolic activity between Iberian and modern pig breeds that might be explained by genotypic differences. Primary hepatocyte cultures are a valuable tool to study specific metabolic liver functions, without the interference of changing levels of metabolites and hormones found in vivo. These...

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“…Previously, our group has developed and successfully implemented a stable isotope method to measure the in vivo fractional synthesis and breakdown rates (FSR 2 and FBR, respectively) of skin protein (1)(2)(3). Using this and other methods, we and others have reported the unique features of skin and wound protein metabolism (1,2,4) and its responses to nutritional and hormonal interventions (3,5,6). Amino acid (AA) supplementations and insulin (INS) are of particular interest among these interventional factors due to their well known anabolic effect on muscle and importance in wound healing (7,8).…”

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Amino Acid Availability Regulates the Effect of Hyperinsulinemia on Skin Protein Metabolism in Pigs

Tuvdendorj¹,

Børsheim²,

Sharp

et al. 2015

Journal of Biological Chemistry

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Background: Insulin is known as an anabolic agent; however, its effect on skin protein metabolism is not well understood. Results: Hyperaminoacidemia alters the effect of hyperinsulinemia on skin protein metabolism in pigs. Conclusion: Hyperinsulinemia itself was not anabolic but rather affected skin amino acid cycling. Significance: Knowing the effect of hyperinsulinemia on skin protein metabolism will help with understanding the pathophysiology of skin conditions.

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Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs

Cited by 79 publications

References 57 publications

Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs

Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs

Metabolic differences in hepatocytes of obese and lean pigs

Amino Acid Availability Regulates the Effect of Hyperinsulinemia on Skin Protein Metabolism in Pigs

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