Differential effects of inhibitors of cell division upon the growth stimulating activities of insulin and serum in nutritionally depleted human and mouse cells

Kamely, Daphne

doi:10.1002/jcp.1040900209

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…In addition, cells in some systems are prevented from entering S-phase. This has been shown in rat liver cells after partial hepatectomy (Luyckx and Van Lancker, 1966;Walker et al, 1977) and in serum-stimulated W138 human, C3H and 3T3 mouse, and chick embryo fibroblasts in vitro (Walker et al, 1977;McClain et al, 1977;Kamely, 1977). Lectininduced lymphocyte mitogenesis is also inhibited (Edelman et al, 1973).…”

Section: Discussionmentioning

confidence: 77%

Effects of cytoskeletal disrupting agents on replication of bovine endothelium

Selden

Rabinovitch

Schwartz

1981

Journal Cellular Physiology

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Colchicine and vinblastine inhibited endothelial cell migration but had no effect on the stimulation of replication seen at wound edges in cultures of endothelium at stationary density. This is in contrast to the effects of cytochalasins which inhibit both migration and replication at wound edges. Moreover, colchicine and vinblastine stimulated cell replication in the unwounded, confluent monolayer. This effect has kinetics similar to the stimulation of replication at a wound edge and is associated with an initial retraction of cell borders, leaving gaps between cells. Cytochalasin D inhibited the growth response to microtubule disrupting agents but did not prevent cell retraction. Stimulation of replication by microtubule disrupting agents was not dependent on serum but was synergistic with serum in cultures rinsed repeatedly with serum-free medium. The replication occurred prior to any cell loss. When, however, cells were allowed to complete mitosis, about one-half of the daughter cells detached from the monolayer so that there was no increase in cell density. We conclude that microtubule disrupting agents are the first agents found to be effective in stimulating growth of vascular endothelium at saturation density. These data further suggest that colchicine and vinblastine stimulate cell growth in a manner similar to wounding, where cell movement is a prerequisite to cell replication.

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Section: Discussionmentioning

confidence: 77%

Effects of cytoskeletal disrupting agents on replication of bovine endothelium

Selden

Rabinovitch

Schwartz

1981

Journal Cellular Physiology

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“…It has also been shown that 3T3 cells can be stimulated to proliferate by insulin if the culture medium is depleted of essential nutrients. This would suggest that the mitogenic action of insulin in this case could be due to the accelerated intracellular transport of some rate-limiting nutrients (23,24) . It follows, therefore, that differentiated PC 13 cells may have specific nutrient requirements for proliferation that can be supplied by insulin-stimulated transport processes .…”

Section: Insulin Effects On Cell Proliferationmentioning

confidence: 93%

Appearance of functional insulin receptors during the differentiation of embryonal carcinoma cells.

Heath¹,

Bell²,

Rees³

1981

The Journal of Cell Biology

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The insulin-receptor binding activity and insulin-stimulated growth response of PC13 clone 5 cells were investigated for both the embryo carcinoma (EC) and retinoic acid-induced differentiated derivatives of this cell line . Whereas the EC cell was found to have very few, if any, receptors and showed no demonstrable dependence on insulin for growth, the differentiated derivative cell expressed a large number of insulin receptors and, when challenged with the hormone, showed stimulation of both DNA synthesis and cell division . The same data were obtained for five independent PC13 clones . These results, coupled with previous observations, lend weight to the suggestion that the appearance of specific receptors for growth regulatory substances may be a manifestation of a general change in growth-regulatory mechanisms accompanying EC cell differentiation and loss of malignancy.

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Responsiveness to insulin is a dominant characteristic in somatic cell hybrids

Straus¹,

Williamson²

1978

Journal Cellular Physiology

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The mouse melanoma cell line PG19 has been found to be unresponsive to the growth-stimulatory action of insulin, although it responds well to other growth factors present in serum. Insulin stimulates DNA synthesis in mouse embryo fibroblasts, and responsiveness to insulin has been found to be a dominant characteristic in mouse fibroblast x PG19 hybrids. To examine the possibility that the unresponsiveness to insulin of the melanoma cells is attributable to a lack of insulin receptors, we have measured the binding of 125I-labeled insulin to the fibroblasts, melanoma cells, and fibroblast x melanoma hybrids. Insulin binds to the surface of the melanoma cells; however, the binding affinity appears to be lower than that observed for binding to diploid fibroblasts. In addition, the dissociation of insulin from the melanoma cells is not accelerated by excess unbound insulin, a kinetic effect observed in the dissociation of insulin from the fibroblasts and fibroblast x melanoma hybrids. This suggests that the class of insulin receptors characterized by this effect is absent on the PG19 cells, and present on the fibroblasts and fibroblast x PG19 hybrids.

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Differential effects of inhibitors of cell division upon the growth stimulating activities of insulin and serum in nutritionally depleted human and mouse cells

Cited by 5 publications

References 31 publications

Effects of cytoskeletal disrupting agents on replication of bovine endothelium

Effects of cytoskeletal disrupting agents on replication of bovine endothelium

Appearance of functional insulin receptors during the differentiation of embryonal carcinoma cells.

Responsiveness to insulin is a dominant characteristic in somatic cell hybrids

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