Differential Effects of Glyphosate and Roundup on Human Placental Cells and Aromatase

Richard, Sophie; Moslemi, Safa; Sipahutar, Herbert; Bénachour, Nora; Séralini, Gilles‐Éric

doi:10.1289/ehp.7728

Cited by 508 publications

(322 citation statements)

References 38 publications

(49 reference statements)

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“…Richard et al (2005) found that the enzyme aromatase, related to reproductive functions, is affected by glyphosate. Similarly, due to its detection at high levels in residential water, diuron was tested for its carcinogenic potential in mammals; in that research, Da Rocha et al (2012) reported that the herbicide causes bladder cancer in mice when mixed in feed at a concentration of 2.500 ppm.…”

Section: Introductionmentioning

confidence: 99%

Herbicide detection in groundwater in Córrego Rico-SP watershed

et al. 2015

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-Due to the large amount of pesticides applied in agriculture, mainly herbicides, there is a growing concern about a possible environmental contamination with these products, including water bodies. Given the above, the aim of the present work was to detect and quantify herbicides through multiresidue analysis in water samples collected in semiartesian wells and springs in a rural area of the city of Jaboticabal (SP). Samples were collected from 32 wells and 13 water springs, in three different seasons: October 2010, February 2011 and May 2011. Additionally, samples at a residence in the urban area were also collected. Analysis using high performance liquid chromatography coupled to mass spectrometry was performed and herbicides ametryn, amicarbazone, clomazone, diclosulan, diuron, hexazinone, imazapic, imazapyr, isoxaflutole, S-metolachlor, sulfentrazone, sulfometuron-methyl, and tebuthiuron were evaluated. In semi-artesian wells, an incresed quantity of herbicides was found in comparison with the water springs. Among the tested herbicides, hexazinone, imazapyr and sulfentrazone were detected in measurable amounts in accordance with the analytical method applied, while clomazone was the most common herbicide being detected in more than 60% of the samples. Ametryn, diuron and amicarbazone herbicides were also detected. Diclosulan, imazapic, isoxaflutole, S-metolachlor, sulfometuron-methyl, and tebuthiuron were not detected in any sample. Inappropriate use of these products without prior knowledge of the behavior of the soil can lead to groundwaters and water springs contamination, thus an ongoing monitoring of this resource becomes very important.Keywords: sugarcane, LC-MS/MS, water springs, semi-artesian wells. ametryn, amicarbazone, clomazone, diclosulan, diuron, hexazinone, imazapic, imazapyr, isoxaflutole, S-metolachlor, sulfentrazone, sulfometuron-methyl SANTOS, E.A. et al. RESUMO -Em vista da grande quantidade de agroquímicos aplicados na agricultura, principalmente os herbicidas, cresce a preocupação com a possível contaminação do ambiente com esses produtos, inclusive em corpos d'água. Diante do exposto, o objetivo deste trabalho foi detectar e quantificar herbicidas, por meio de análise multirresíduo, em amostras de água coletadas em poços semiartesianos e nascentes, em uma área rural da cidade de Jaboticabal (SP). Foram coletadas amostras em 32 poços e 13 nascentes, em três épocas: outubro de 2010, fevereiro e maio de 2011. Foram coletadas, adicionalmente, amostras em uma residência na área urbana. Foi realizada análise por cromatografia líquida de alta eficiência acoplada à espectrometria de massas e foram avaliados os herbicidas

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Section: Introductionmentioning

confidence: 99%

Herbicide detection in groundwater in Córrego Rico-SP watershed

et al. 2015

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“…From a human health perspective, glyphosate is classified as carcinogenic category E because of evidence of noncarcinogenicity for humans [21]. Some studies have suggested a link between glyphosate exposure and the risk of non-Hodgkin's lymphoma or human placental cell damage [22,23].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and fate of the herbicide glyphosate and its degradate aminomethylphosphonic acid in the atmosphere

Chang

Simcik

Capel

2011

Enviro Toxic and Chemistry

134

106

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Abstract-This is the first report on the ambient levels of glyphosate, the most widely used herbicide in the United States, and its major degradation product, aminomethylphosphonic acid (AMPA), in air and rain. Concurrent, weekly integrated air particle and rain samples were collected during two growing seasons in agricultural areas in Mississippi and Iowa. Rain was also collected in Indiana in a preliminary phase of the study. The frequency of glyphosate detection ranged from 60 to 100% in both air and rain. The concentrations of glyphosate ranged from <0.01 to 9.1 ng/m 3 and from <0.1 to 2.5 mg/L in air and rain samples, respectively. The frequency of detection and median and maximum concentrations of glyphosate in air were similar or greater to those of the other high-use herbicides observed in the Mississippi River basin, whereas its concentration in rain was greater than the other herbicides. It is not known what percentage of the applied glyphosate is introduced into the air, but it was estimated that up to 0.7% of application is removed from the air in rainfall.

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“…Diferentes estudios in vitro han mostrado que el glifosato es citotóxico para células tanto humanas como de otros animales (24,25,27). Aunque los mecanismos específicos de citotoxicidad se desconocen, éstos bien pudieran involucrar cambios en la permeabilidad de las membranas o en la actividad de enzimas mitocondriales que conducirían a la muerte celular (28).…”

Section: Discussionunclassified

“…Los resultados presentados aquí son consistentes con otros estudios in vitro que han comparado los efectos tóxicos del glifosato grado técnico y de las formulaciones comerciales, los cuales han indicado que estas últimas resultan más tóxicas que el primero; por ejemplo, la adición de 0,1% de Roundup® al glifosato grado técnico redujo significativamente la viabilidad de las células placentarias humanas JEG3 (25).…”

Section: Figuraunclassified

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Citotoxicidad del glifosato en células mononucleares de sangre periférica humana

Martínez

Reyes

2007

biomedica

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Introducción. El glifosato es un herbicida de amplio espectro, no selectivo, utilizado comúnmente en agricultura para eliminar malezas. Los estudios que han evaluado la toxicidad del glifosato en animales y en ambiente muestran que las formulaciones comerciales son más tóxicas que el componente activo. Objetivos. Evaluar la toxicidad del glifosato grado técnico y de la formulación comercial Roundup® en células mononucleares de sangre periférica humana. Materiales y métodos. Células mononucleares de sangre periférica humana fueron expuestas a diferentes concentraciones de glifosato en grado técnico y en la forma de Roundup® por 24, 48, 72 y 96 horas. La citotoxicidad se evaluó mediante el método de exclusión con azul de tripano y reducción del reactivo sal sódica de (2,3-bis[2-metoxi-4-nitro-5-sulfofenil]-2Htetrazolio-5-carboxianilida) (XTT). Resultados. Ambas presentaciones del glifosato (grado técnico y Roundup®) fueron tóxicas para las células mononucleares de sangre periférica humana. Roundup® fue más citotóxico que el glifosato grado técnico, ya que se encontró que la concentración letal 50 (LC 50 ) analizada con el método de exclusión con azul de tripano a las 24 horas fue de 56,4 µg/ml de glifosato en la forma de Roundup® y de 1.640 mg/ml (1,64 µg/ml) para glifosato grado técnico. Conclusiones. Los resultados de este estudio in vitro confirman el efecto tóxico para las células humanas observado para el glifosato y sus preparaciones comerciales, y que estas últimas son más citotóxicas que el compuesto activo, lo que apoya la idea de que los aditivos presentes en las formulaciones comerciales juegan un papel crucial en la toxicidad atribuida a los herbicidas que contienen glifosato.Palabras clave: pruebas inmunológicas de citotoxicidad, herbicidas, células sanguíneas, supervivencia celular, azul de tripano, sales de tetrazolio. Cytotoxicity of the herbicide glyphosate in human peripheral blood mononuclear cellsIntroduction. Glyphosate is a broad-spectrum, non-selective herbicide and commonly used to eliminate weeds in agricultural and forest settings. Studies evaluating glyphosate toxicity in animals and environment show that commercial formulations of glyphosate are more toxic than the active component itself. Objectives. Technical grade glyphosate was compared with the commercial formulation Roundup® in their respective toxicities on human peripheral blood mononuclear cells. Materials and methods. Human peripheral blood mononuclear cells were exposed to different concentrations of glyphosate, either technical grade or in the form of Roundup for 24 h, 48 h, 72 h, and 96 h. Cytotoxicity was assayed by trypan blue dye exclusion method and reduction of (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2Htetrazolium-5-carboxyanilide inner salt) XTT reagent. Results. Both technical grade glyphosate and Roundup® formulation were toxic to human peripheral blood mononuclear cells. Cytotoxicity of Roundup® was higher than cytotoxicity of glyphosate, since the LC 50 (50% lethal concentration) determined by the trypan blue ex...

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Differential Effects of Glyphosate and Roundup on Human Placental Cells and Aromatase

Cited by 508 publications

References 38 publications

Herbicide detection in groundwater in Córrego Rico-SP watershed

Herbicide detection in groundwater in Córrego Rico-SP watershed

Occurrence and fate of the herbicide glyphosate and its degradate aminomethylphosphonic acid in the atmosphere

Citotoxicidad del glifosato en células mononucleares de sangre periférica humana

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