Differential effects of dopamine D1 and D2/3 receptor antagonism on motor responses

Tran, Steven; Nowicki, Magda; Muraleetharan, Arrujyan; Gerlai, Robert

doi:10.1007/s00213-014-3713-0

Cited by 31 publications

(19 citation statements)

References 51 publications

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“…Acute application of 5 μM L741,626, a D 2 ‐specific receptor antagonist, increased the occurrence of swimming episodes in 72 hpf ZF . Conversely, adult ZF treated with 0.27 μM amisulpride, a D 2/3 receptor antagonist, moved less than fish treated with 2.7 μM amisulpride . The authors proposed that, at the higher dose of 2.7 μM, blockage of postsynaptic D 2 receptors occurs and leads to disinhibition (ie, more movement).…”

Section: Discussionmentioning

confidence: 99%

Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

Shontz

Souders

Schmidt

et al. 2018

Genes Brain and Behavior

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Dopamine (DA) plays a significant role in cognition, motor function and social behavior. The objectives of this study were to (1) quantify the temporal expression of transcripts (DA receptors, transporters and tyrosine hydroxylase) associated with DA signaling during early stages of zebrafish development and (2) determine their expression profiles following treatment with a D receptor antagonist domperidone (DMP). We also assessed locomotor behavior following treatment with DMP using alternating periods of light and dark (ie, dark photokinesis), as DA plays a key role in behavior. Relative expression levels of transcripts that were investigated and related to the DA system were detected after the first 24 hours postfertilization (hpf). Some DA receptor transcripts (eg, drd4c) increased in abundance earlier in the embryo compared with other receptors (eg, drd3), suggesting that DA receptor paralogs may have unique roles in development. Treatment of larvae with DMP resulted in the upregulation of DA receptor transcripts (ie, drd1, drd7, drd4b, drd4c) and DA transporter 1 (ie, slc6a3), and it is hypothesized that upregulation of genes related to the DA system is a compensatory neurophysiological response to DA receptor antagonism. Larval activity during dark photokinesis (measured by distance traveled) was also elevated by DMP. We hypothesize that behavioral responses observed with DMP may be related to the regulation of deep brain photoreception in zebrafish (Danio rerio) (ZF) larvae by DA.

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Section: Discussionmentioning

confidence: 99%

Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

Shontz

Souders

Schmidt

et al. 2018

Genes Brain and Behavior

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“…This hypothesis is supported by studies involving the inhibition of dopaminergic receptors. For example, antagonism of D 1 receptors, the most abundant dopaminergic receptor subtype in the zebarfish brain, reduces baseline motor activity in zebrafish (Tran et al, 2015b). …”

Section: Discussionmentioning

confidence: 99%

“…Immersion-based drug delivery was used because of its less invasive nature compared to other methods of administration (e.g. injection) and frequent use in adult zebrafish research (Nowicki et al, 2014; Tran et al, 2015b; Levin et al, 2007; Conners et al, 2014; Sackerman et al, 2010). …”

Section: Methodsmentioning

confidence: 99%

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Inhibition of phosphorylated tyrosine hydroxylase attenuates ethanol-induced hyperactivity in adult zebrafish (Danio rerio)

Nowicki

Tran

Chatterjee

2015

Pharmacology Biochemistry and Behavior

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Zebrafish have been successfully employed in the study of the behavioural and biological effects of ethanol. Like in mammals, low to moderate doses of ethanol induce motor hyperactivity in zebrafish, an effect that has been attributed to the activation of the dopaminergic system. Acute ethanol exposure increases dopamine (DA) in the zebrafish brain, and it has been suggested that tyrosine hydroxylase, the rate-limiting enzyme of DA synthesis, may be activated in response to ethanol via phosphorylation. The current study employed tetrahydropapaveroline (THP), a selective inhibitor of phosphorylated tyrosine hydroxylase, for the first time, in zebrafish. We treated zebrafish with a THP dose that did not alter baseline motor responses to examine whether it can attenuate or abolish the effects of acute exposure to alcohol (ethanol) on motor activity, on levels of DA, and on levels of dopamine’s metabolite 3,4-dihydroxyphenylacetic acid (DOPAC). We found that 60-minute exposure to 1% alcohol induced motor hyperactivity and an increase in brain DA. Both of these effects were attenuated by pre-treatment with THP. However, no differences in DOPAC levels were found among the treatment groups. These findings suggest that tyrosine hydroxylase is activated via phosphorylation to increase DA synthesis during alcohol exposure in zebrafish, and this partially mediates alcohol’s locomotor stimulant effects. Future studies will investigate other potential candidates in the molecular pathway to further decipher the neurobiological mechanism that underlies the stimulatory properties of this popular psychoactive drug.

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“…We conducted a temporal analysis (1 min intervals) and an endpoint analysis (last 10 min) for the total distance traveled during both exposures. The last 10 min of exposure was chosen since behavioral responses during this period were found most robust and stable following exposure to different pharmacological compounds [24,25]. Fig.…”

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confidence: 99%

MK-801 increases locomotor activity in a context-dependent manner in zebrafish

Tran

Muraleetharan

Fulcher

et al. 2016

Behavioural Brain Research

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Differential effects of dopamine D1 and D2/3 receptor antagonism on motor responses

Abstract: The results suggest that zebrafish, a cheaper and simpler model organism compared to the rat and the mouse, may be an efficient translationally relevant tool for the analysis of the psychopharmacology of receptors of the vertebrate dopaminergic system.

Cited by 31 publications

References 51 publications

Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

Inhibition of phosphorylated tyrosine hydroxylase attenuates ethanol-induced hyperactivity in adult zebrafish (Danio rerio)

MK-801 increases locomotor activity in a context-dependent manner in zebrafish

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