Differential effects of bismuth and salicylate salts on the antibiotic susceptibility ofPseudomonas aeruginosa

Domenico, Philip; O’Leary, Ryan; Cunha, Burke A.

doi:10.1007/bf01967072

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Using BisEDT in combination with Cz may have disturbed efflux pumps and increased bacterial permeability which leads to an increase in intercellular concentration of Cz. According to Domenico et al bismuth can augment the Cz effect on P. aeruginosa and in our study similar results were observed in the biofilm inhibition assays (7). Loss of OprD, a porin that forms narrow transmembrane channels, is an important mechanism of resistance to imipenem (20).…”

Section: Discussionsupporting

confidence: 91%

“…The thiol element functions as a lipophilic carrier that enhances bismuth uptake through bacterial surface up to 1000-fold (6). Bismuth acts as a metabolic poison inside the cell, results in growth inhibition and cell death (7). At sub inhibitory concentrations, BTs inactivate the bacterial respiratory enzymes, suppress the exopolysaccharide expression and inhibit biofilm formation in Gram-positive and -negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Effects of Bismuth Thiols and Various Antibiotics Against Pseudomonas aeruginosa Biofilm

Varposhti

Ali

Mohammadi

2014

Jundishapur J Microbiol

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Background:Pseudomonas aeruginosa is an opportunistic pathogen that takes advantages of some weaknesses in the immune system to initiate an infection. Biofilms of P. aeruginosa can cause chronic opportunistic infections in immunocompromised and elderly patients. This bacterium is considered as a model organism to study antibiotic resistance as well as biofilm formation. In the biofilm structures, bacteria are protected from many harmful environmental factors such as fluctuations in the level of oxygen and nutrients, and the alterations of pH as well as sensitivity to antibiotics. Decreased permeability of biofilms is one of the important reasons of antimicrobial resistance in bacteria.Objectives:In this study the anti-biofilm activity of bismuth thiols in combination with ciprofloxacin, imipenem and ceftazidime against the P. aeruginosa biofilm was investigated.Materials and Methods:Checkerboard method was used to test the susceptibility of biofilms against various antimicrobial combinations. The biofilm formation was measured by 2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) colorimetric assay. The fractional bio-film inhibitory concentration was reported for each agent.Results:The combination of bismuth ethanedithiol with ciprofloxacin showed synergistic inhibitory effect on the P. aeruginosa biofilm formation. The combination of bismuth ethanedithiol ciprofloxacin, ceftazidime and imipenem showed synergistic inhibitory effects on the biofilm formation. Furthermore, the combination of bismuth ethanedithiol, imipenem and ceftazidime did not show any synergistic inhibitory effect on biofilm formation.Conclusions:Our studies show that using appropriate concentrations of bismuth thiols in combination with various antibiotics can act synergistically against P. aeruginosa biofilm formation.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Bismuth Thiols and Various Antibiotics Against Pseudomonas aeruginosa Biofilm

Varposhti

Ali

Mohammadi

2014

Jundishapur J Microbiol

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“…Reversible resistance to chloramphenicol, ampicillin, tetracycline and nalidixic acid was described for Gram-negative bacilli grown in salicylate (11). Other reports have described several in vitro effects of salicylate on bacteria including the reduction of surface polysaccharides, potentiation of amino-glycoside activity and antagonism of~-lactam anti-biotic activity (12,14). On the other hand, salicylates increased resistance level of E. coli strains to ampicillin, chloramphenicol, nalidixic acid and tetracyclines and of Pseudomonas aeruginosa and Pseudomonas cepacia by decreasing membrane permeability (14)(15)(16).…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetic parameters and killing rates in serum of volunteers receiving amoxicillin, cefadroxil or cefixime alone or associated with niflumic acid or paracetamol

Carsenti-Etesse

Farinotti

Durant

et al. 1998

Eur. J. Drug Metab. Pharmacokinet.

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Pharmacokinetic parameters and killing rates in serum of volunteers receiving amoxicillin, cefadroxil or cefixime alone or associated with niflumic acid or paracetamol were studied. Niflumic acid (250 mg) or analgesic and antipyretic drugs such as paracetamol (500 mg) are often combined with antibiotics to avoid inflammation and pain in acute ear, nose and throat diseases. Pharmacokinetic interactions between these two classes of drugs have been described in experimental models, and exceptionally in humans. The aim of the present investigation was to study the interactions of these two drugs with three antibiotics (amoxicillin 500 mg x 2, cefadroxil 500 mg x 2, cefixime 200 mg and one placebo capsule) on pharmacodynamic parameters and on rate of killing in the serum of six healthy volunteers receiving the antibiotic associated or not with the product in a randomized cross-over double-blind trial. The bacteria most often involved in sinusitis, bronchitis and otitis media (Haemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus) three target diseases for oral cephalosporins and amoxicillin, were chosen for bacteriological study. Blood samples were obtained at 0.25, 0.50, 1, 1.5, 2, 4, 6 and 12 h after oral administration of antibiotics alone or associated with the drugs. There was a wash-out period of at least 1 week between the eleven sequences. Antibiotics were measured by two methods: bioassay and high performance liquid chromatography (HPLC). All serum samples obtained at peak level, 4 and 6 h were tested for killing rate. Area under the time kill curve was calculated by the trapezoidal rule method and relative bioactivity in percent was defined as follows: (AUC control - AUC test)/AUC control x 100. No pharmacokinetic interaction was found in the AUC and T1/2 of the plasma concentrations of the antibiotics or associated with the drugs, regardless of dose, as determined by HPLC or microbiological assay. For these beta-lactam antibiotics killing rate was found to be time-dependent. Bactericidal activity was improved on H. influenzae when cefixime was associated with niflumic acid and became concentration-dependent. A significant concentration relation was also found with niflumic acid or paracetamol associated with cefixime on Strep. pneumoniae.

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“…Bismuth is a compound that has been used for years to treat gastrointestinal ulcers due to Helicobacter pylori, although it has also demonstrated activity against Gram-negative organisms, reduced P. aeruginosa biofilms, and acted synergistically with tobramycin against P. aeruginosa (146)(147)(148)(149)(150). Despite these advantageous properties, low concentrations of bismuth have been shown to be toxic to human lung cells (151).…”

Section: Animalsmentioning

confidence: 99%

Inhaled Antibiotics for Gram-Negative Respiratory Infections

et al. 2016

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SUMMARYGram-negative organisms comprise a large portion of the pathogens responsible for lower respiratory tract infections, especially those that are nosocomially acquired, and the rate of antibiotic resistance among these organisms continues to rise. Systemically administered antibiotics used to treat these infections often have poor penetration into the lung parenchyma and narrow therapeutic windows between efficacy and toxicity. The use of inhaled antibiotics allows for maximization of target site concentrations and optimization of pharmacokinetic/pharmacodynamic indices while minimizing systemic exposure and toxicity. This review is a comprehensive discussion of formulation and drug delivery aspects,in vitroand microbiological considerations, pharmacokinetics, and clinical outcomes with inhaled antibiotics as they apply to disease states other than cystic fibrosis. In reviewing the literature surrounding the use of inhaled antibiotics, we also highlight the complexities related to this route of administration and the shortcomings in the available evidence. The lack of novel anti-Gram-negative antibiotics in the developmental pipeline will encourage the innovative use of our existing agents, and the inhaled route is one that deserves to be further studied and adopted in the clinical arena.

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Differential effects of bismuth and salicylate salts on the antibiotic susceptibility ofPseudomonas aeruginosa

Cited by 12 publications

References 22 publications

Synergistic Effects of Bismuth Thiols and Various Antibiotics Against Pseudomonas aeruginosa Biofilm

Synergistic Effects of Bismuth Thiols and Various Antibiotics Against Pseudomonas aeruginosa Biofilm

Pharmacokinetic parameters and killing rates in serum of volunteers receiving amoxicillin, cefadroxil or cefixime alone or associated with niflumic acid or paracetamol

Inhaled Antibiotics for Gram-Negative Respiratory Infections

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