Differential effects of Bacillus subtilis– and Bacillus licheniformis–fermented products on growth performance, intestinal morphology, intestinal antioxidant and barrier function gene expression, cecal microbiota community, and microbial carbohydrate-active enzyme composition in broilers

Chen, Yuwei; Yu, Yu-Hsiang

doi:10.1016/j.psj.2023.102670

Cited by 7 publications

(11 citation statements)

References 38 publications

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“…Our observations of the abundance of individual antibiotic resistance classes and individual ARGs were different to those in our previous study (Chen and Yu, 2022b). Similar to our previous study (Chen and Yu, 2020), the growth-promoting effect of LFP in broilers was also observed in the current study (these data are presented by Chen and Yu, 2023), although the concentration of B. licheniformis spore was different. However, the cecal microbiota composition of broilers in response to LFP supplementation was slightly different in these two studies (Chen and Yu, 2020;Chen and Yu, 2023).…”

Section: Discussionsupporting

confidence: 91%

“…Similar to our previous study (Chen and Yu, 2020), the growth-promoting effect of LFP in broilers was also observed in the current study (these data are presented by Chen and Yu, 2023), although the concentration of B. licheniformis spore was different. However, the cecal microbiota composition of broilers in response to LFP supplementation was slightly different in these two studies (Chen and Yu, 2020;Chen and Yu, 2023). The different dominant genera with assigned antibiotic resistance classes and ARGs may explain the inconsistent effects on the cecal antibiotic resistome in these studies.…”

Section: Discussionsupporting

confidence: 91%

“…This research was approved by the Institutional Animal Care and Use Committee of National Ilan University (109-26). The detailed preparation and composition of SFPs and LFPs were described previously (Chen and Yu, 2023). We randomly allocated 160 1-day-old unsexed Arbor Acres broiler chicks to four treatment groups, with eight replicates containing five chicks per replicate.…”

Section: Experimental Designmentioning

confidence: 99%

“…B. subtilis and B. licheniformis have distinct antimicrobial mechanisms (Tran et al, 2022). We previously demonstrated that SFPs and LFPs differentially modulate the cecal microbial community of broilers (Chen and Yu, 2023). However, the differential effect of these two solid-state fermented products on ARG and VFG diversity and distribution from the gut of broilers, as determined using metagenomic sequencing, remains unclear.…”

mentioning

confidence: 99%

“…In this study, we used metagenomic sequencing to evaluate the effect of Bacillus species -fermented products on antibiotic resistome and VFG composition in the gut microbiota of broilers. On the basis of our previous findings that SFPs and LFPs differentially regulate broiler growth performance and gut microbiota (Chen and Yu, 2023), we examined the ARG and VFG composition in the cecal digesta of broilers in the present study.…”

mentioning

confidence: 99%

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Differential Effects of Bacillus Species-Fermented Products on Antibiotic Resistome and Virulence Factor Gene Composition in the Cecal Digesta of Broilers

Hsiao,

Cheng,

Chen

et al. 2024

Annals of Animal Science

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This study investigated the differential effects of Bacillus subtilis – and Bacillus licheniformis – fermented products (defined as SFP and LFPs, respectively) on microbial antibiotic resistance gene (ARG) and virulence factor gene (VFG) composition in the cecal digesta of 35-day-old broilers by using metagenomic sequencing. First, 160 1-day-old unsexed Arbor Acres broiler chicks were randomly allocated to four treatment groups: basal diet (CON group); basal diet + enramycin (10 mg/kg, ENM group); basal diet + SFPs (108 colony-forming units (CFU) of B. subtilis spores/kg, SFP group); and basal diet + LFPs (108 CFU of B. licheniformis spores/kg, LFP group). Principal coordinate analysis of ARG and VFG composition indicated distinct clustering among the cecal samples of the groups. At the antibiotic resistance class level, LFP treatment increased the expression of peptide resistance genes and decreased the expression of aminocoumarin resistance genes compared with the other groups. Compared with the other groups, LFP treatment promoted bcrA, ugd, and efrB expression but suppressed parY expression, whereas SFP treatment inhibited efrA expression. The abundance of the peptide resistance gene bcrA in Lachnoclostridium species was higher in the CON and LFP groups than in the ENM and SFP groups, whereas the abundance of the peptide resistance gene rpoB2 in Bacteroides species was lower in the ENM and LFP groups than in the SFP group. No specific VFGs were regulated only by SFPs or LFPs. SFP and LFP treatment inhibited clpC expression compared with the other groups. clpC abundance in Bacteroides species was lower in the LFP group than in the CON group, whereas its abundance in Faecalibacterium species was lower in the SFP group than in the CON and ENM groups. These results demonstrated that SFPs and LFPs differentially regulate microbial ARG and VFG composition in the cecal digesta of broilers. LFP supplementation modulated more antibiotic resistance classes and ARGs than did SFP supplementation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Experimental Designmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Differential Effects of Bacillus Species-Fermented Products on Antibiotic Resistome and Virulence Factor Gene Composition in the Cecal Digesta of Broilers

Hsiao,

Cheng,

Chen

et al. 2024

Annals of Animal Science

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Effects of cyclic antimicrobial lipopeptides from Bacillus subtilis on growth performance, intestinal morphology, and cecal gene expression and microbiota community in broilers

Chen,

2024

Animal Science Journal

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This study investigated the effects of cyclic antimicrobial lipopeptides (CLPs) from Bacillus subtilis on the growth performance, gut morphology, and cecal gene expression and microbiota in broilers; 120 1‐day‐old unsexed Arbor Acres chicks were randomly divided into four groups, with six replicates in each group and five broilers per cage. These groups were fed a basal diet (C), basal diet plus 10‐mg enramycin/kg (E), and basal diet plus 51‐mg CLPs/kg (L) or 102‐mg CLPs/kg (H). The results indicated that CLP supplementation linearly increased the body weight compared with the C group at 35 days of age. Between 15 and 35 days and 1 and 35 days of age, CLP supplementation linearly increased the average daily gain compared with the C group. The duodenal villus height was significantly increased in the H group compared with the C and E groups. In the cecum, CLP supplementation linearly increased SOD and ZO‐1 mRNA expression compared with the C group. β diversity of microbiota indicated distinct clusters between the groups. CLP supplementation linearly increased the abundance of the genus Lactobacillus in the cecal digesta compared with the C group. These results demonstrate that B. subtilis–produced CLPs dose‐dependently increase broilers' growth performance, improve their gut morphology, and modulate their gut microbiota.

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Determination of Antagonistic Properties and Biofilm Formation in Bacillus Spp. And Lactobacillus Spp.

Bezpalko,

Machuskyi,

Vygovska

et al. 2023

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursìv ì priro

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The aim of the work was the selection of cultures of Bacillus spp. and Lactobacillus spp. promising for use as probiotics. Samples of chicken droppings (250 samples) for research were collected in the period of 2020 from clinically healthy chickens (age 28-48 days). in poultry farms of various scales and methods of keeping poultry (large-scale industrial farms, farms and homestead farms) in Vinnytsia, Zhytomyr, Kyiv, Kharkiv, Cherkasy, and Chernihiv regions. According to the results of bacteriological studies, 94 cultures of the genus Bacillus (B. subtilis, B. licheniformis, B. cereus, B. megaterium, B. pumilus) and 56 isolates of the genus Lactobacillus (L. delbrueckii, L. Lactis, L. Reuteri, L. casei, L. brevis). In general, 32.1 % of the studied isolates of Lactobacillus spp. and 21.3% of isolates of Bacillus spp. were able to form high-density biofilms in vitro. The results of determining the antagonistic properties showed that 98.4% and 91.5% of isolates of the genus Lactobacillus and the genus Bacillus respectively showed medium and high antagonistic activity against 17 test strains of microorganisms. The obtained results regarding the ability of the studied isolates to form a biofilm, antagonistic action, resistance to the aggressive environment of the digestive tract (pH level, bile) became the basis for the selection of promising cultures of the genus Bacillus and Lactobacillus for the purpose of developing a preparation with probiotic action. Further research will be aimed at determining the resistance of selected isolates to antimicrobial drugs and, subsequently, determining the effectiveness of selected isolates as part of a complex probiotic in experiments on chickens with the aim of evaluating their immunocorrective and antioxidant effects, as well as preventing the development of intestinal infections as an alternative to the use of antimicrobials drugs.

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Differential effects of Bacillus subtilis– and Bacillus licheniformis–fermented products on growth performance, intestinal morphology, intestinal antioxidant and barrier function gene expression, cecal microbiota community, and microbial carbohydrate-active enzyme composition in broilers

Cited by 7 publications

References 38 publications

Differential Effects of Bacillus Species-Fermented Products on Antibiotic Resistome and Virulence Factor Gene Composition in the Cecal Digesta of Broilers

Differential Effects of Bacillus Species-Fermented Products on Antibiotic Resistome and Virulence Factor Gene Composition in the Cecal Digesta of Broilers

Effects of cyclic antimicrobial lipopeptides from Bacillus subtilis on growth performance, intestinal morphology, and cecal gene expression and microbiota community in broilers

Determination of Antagonistic Properties and Biofilm Formation in Bacillus Spp. And Lactobacillus Spp.

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