Differential effects of alcohol upon gluconeogenesis from lactate in young and old hepatocytes

Sumida, Ken D.; Crandall, Suzanne C.; Chadha, Puja; Qureshi, Tauseef

doi:10.1016/j.exger.2005.01.005

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…[ 72 ] showed that the in vitro inhibitory effect of gluconeogenesis by alcohol was especially noted in matured hepatocytes, than in young ones. [ 72 ] Hence, it would be expected that the effect of alcohol consumption on gluconeogenesis would be more pronounced for the older humans, compared to young adults. This issue of the age differences in alcohol action on liver gluconeogenesis needs further attention.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, two parallel data sources were utilized: Our research data;[ 18 ] and peer-reviewed articles from scientific databases. [ 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 ]…”

Section: Materials and Methods Of Literature Searchmentioning

confidence: 99%

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Glycemic allostasis during mental activities on fasting in non-alcohol users and alcohol users with different durations of abstinence

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Переверзев²

2014

Ann Med Health Sci Res

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Glycemic allostasis is the process by which blood glucose stabilization is achieved through the balancing of glucose consumption rate and release into the blood stream under a variety of stressors. This paper reviews findings on the dynamics of glycemic levels during mental activities on fasting in non-alcohol users and alcohol users with different periods of abstinence. Referred articles for this review were searched in the databases of PubMed, Scopus, DOAJ and AJOL. The search was conducted in 2013 between January 20 and July 31. The following keywords were used in the search: alcohol action on glycemia OR brain glucose OR cognitive functions; dynamics of glycemia, dynamics of glycemia during mental activities; dynamics of glycemia on fasting; dynamics of glycemia in non-alcohol users OR alcohol users; glycemic regulation during sobriety. Analysis of the selected articles showed that glycemic allostasis during mental activities on fasting is poorly regulated in alcohol users even after a long duration of sobriety (1-4 weeks after alcohol consumption), compared to non-alcohol users. The major contributor to the maintenance of euglycemia during mental activities after the night's rest (during continuing fast) is gluconeogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Methods Of Literature Searchmentioning

confidence: 99%

Glycemic allostasis during mental activities on fasting in non-alcohol users and alcohol users with different durations of abstinence

Welcome¹,

Переверзев²

2014

Ann Med Health Sci Res

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“…4a). The liver is the major organ involved in biotransforming galactose into glucose (Xu and Purcell 2006), and this function is retained in liver spheroids; furthermore, glucose can be synthesized from other sources such as pyruvate , lactate (Sumida et al 2005), as well as from a range of glucogenic amino acids such as serine, threonine, glutamine, etc. through gluconeogenesis (Brosnan 2003).…”

Section: Discussionmentioning

confidence: 99%

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies

Bazou

2009

Cell Biol Toxicol

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This paper describes the alginate encapsulation of preformed high-density 3-D HepG2 cell aggregates that guarantees good maintenance of liver-specific biomarker expression. The process involves forming a high-density (> or =7 x 10(4) cells/aggregate) discoid 3-D cell aggregate in an ultrasound trap, which is subsequently recovered and encapsulated in alginate/CaCl(2) hydrogel. Glucose secretion/consumption, lactate release, detoxifying enzyme capacity, cytokeratin-18 expression as well as hypoxia were characterized in encapsulated 3-D HepG2 aggregates over 10 days in culture. Encapsulated 3-D HepG2 aggregates released glucose into the media, although this ability was exhibited only after 1 day in culture and was subsequently lost over the ensuing 9 days. In contrast, lactate was constantly released into the media. Significantly more lactate was secreted after 3 days in culture indicating a more hypoxic environment and hence a higher rate of anaerobic glycolysis. Aggregates consistently expressed cytokeratin-18. Cytochrome P450-1A1 activity reached a maximum on day 1 of culture followed by a progressive reduction to basal levels, while P450-3A4 activity was up-regulated in a time-dependent manner reaching a peak on day 7 in culture. Glutathione-S-transferase activity, on the other hand, was at more physiological levels and remained constant over the 10-day culture period. The ultrasound trap allowed the rapid (within 5 min) generation of uniformly shaped and sized aggregates. The results reported here suggest that ultrasound-formed 3-D HepG2 aggregates can serve as alternative in vitro models providing a quick outlook on toxicity, in a tissue-mimetic manner, thus offering the future option of a cost-effective screening platform for pharmaceutical development.

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“…Before entering the liver, the perfusate was sequentially filtered through a nylon mesh, oxygenated (95%:5%, O 2 :CO 2 ), and then passed through a bubble trap. Hepatocytes were isolated as previously reported by our laboratory (Sumida et al, 2005b) where calcium-free Krebs-Hensleit bicarbonate buffer and fresh thoroughly washed bovine red blood cells (hematocrit of 13-15%) perfused the liver (single-pass) for 10 minutes at a rate of 35 ml/min. Collagenase was added to the perfusate and the system was switched to a recirculation mode with the flow rate adjusted to ∼2 ml/min × g liver −1 .…”

Section: Isolation Of Hepatocytes and Experimentsmentioning

confidence: 99%

Alcohol-induced suppression of gluconeogenesis is greater in ethanol fed female rat hepatocytes than males

Sumida

Cogger

Matveyenko

2007

Alcohol

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The impact of alcohol-induced suppression on hepatic gluconeogenesis (HGN) after chronic ethanol consumption between males and females is unknown. To determine the effects of chronic alcohol consumption (8 weeks) on HGN, the isolated hepatocyte technique was used on 24 h fasted male and female Wistar rats. Livers were initially perfused with collagenase and the hepatocytes were isolated. Aliquots of the cell suspension were placed in Krebs-Henseleit buffer and incubated for 30 min with lactate, [U -14C]lactate, and nine different concentrations of ethanol (EtOH). Dose-effect curves were generated for the determination of maximal and half-maximal alcohol-induced inhibition on HGN. There was no significant difference in HGN (lactate only and no EtOH) between males and females fed the control diet (88.5 +/- 5.1 nmol/mg protein/30 min). Similarly, the HGN (lactate only and no EtOH) in males fed the ethanol diet (ME) were not significantly different (82.8 +/- 3.5 nmol/mg protein/30 min) compared to controls. In contrast, the females chronically fed the ethanol diet (FE) had significantly (P < .05) lower HGN (67.8 +/- 4.6 nmol/mg protein/30 min) compared to both ME and controls. With alcohol in the incubation medium, the HGN significantly (P<.05) declined in all groups. While alcohol suppressed HGN to a larger (P < .05) extent in ME (45.8 +/- 3.7 nmol/mg protein/30 min) compared to controls (64.0 +/- 3.8 nmol/mg protein/30 min), the inhibition was even greater (P < .05) in FE (32.7 +/- 3.2 nmol/mg protein/30 min). The more pronounced effect of chronic alcohol consumption on HGN in the presence of ethanol in female hepatocytes was supported by the concomitant decreases (P < .05) in 14C-lactate incorporation into 14C-glucose, lactate uptake, and 14C-lactate uptake. The results suggest that chronic alcohol consumption elicits a greater reduction on HGN in the presence of ethanol in the hepatocytes of females compared to males.

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Differential effects of alcohol upon gluconeogenesis from lactate in young and old hepatocytes

Cited by 7 publications

References 22 publications

Glycemic allostasis during mental activities on fasting in non-alcohol users and alcohol users with different durations of abstinence

Glycemic allostasis during mental activities on fasting in non-alcohol users and alcohol users with different durations of abstinence

Biochemical properties of encapsulated high-density 3-D HepG2 aggregates formed in an ultrasound trap for application in hepatotoxicity studies

Alcohol-induced suppression of gluconeogenesis is greater in ethanol fed female rat hepatocytes than males

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