Differential Effect of Bursectomy on Antibody Production in a Large and Small Bursa Line of New Hampshire Chickens

Development of the bursa of Fabricius in the chicken was studied from day 4 to day 84 after hatching using light microscopic, morphometric methods. The proportion of lymphoid follicles of the total bursal tissue increased from 62% at day 4 to 86% at day 63 and thereafter decreased slightly until day 84. The corresponding values for bursal stroma and epithelium decreased from day 4 to day 84. The relative and absolute amounts of lymphoid tissue increased from enlargement of individual lymphoid follicles; their mean spatial diameter increased from 185 microns at day 4 to 720 microns at day 84. The number of areas of follicle-associated epithelium at the bursal mucosa, which in a mature bursa corresponds to the follicle number, remained relatively unchanged (range 7200 to 12000 per bursa). the follicular cortex grew more rapidly than did the follicular medulla, indicating different functions for each. The total surface area of the follicle-associated epithelium was .88 cm2 at day 4 increasing to 4.71 cm2 at day 63. This increase also in mainly due to increased size of the individual areas of follicle-associated epithelium (diameter increased from 71 to 168 microns). Histoquantitative findings are discussed in light of present knowledge about the function of the bursa as a central and peripheral lymphoid organ.

Section: Resultssupporting

confidence: 93%

Postembryonic Development of the Chicken Bursa of Fabricius: A Light Microscopic Histoquantitative Study

Romppanen

1982

“…These two lines differ significantly in bursa size at hatch and 1,3, and 5 weeks of age (Glick and Dreesen, 1967;Landreth and Glick, 1973). Surgical bursectomy of the SBL chicks at time of hatch abrogated their hemagglutinin response to sheep-red-blood cells (SRBC) (Landreth and Glick, 1973).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Immune Response Between Two Lines of Chickens Selected for Differences in the Weight of the Bursa of Fabricius

Yamamoto

Glick

1982

Experiments were designed to compare antibody-mediated immunity in two lines of 5-month-old chickens previously selected for a large or small bursa. After receiving a primary or secondary challenge with sheep-red-blood cells, bovine serum albumin, or dinitrophenyl, birds from the small bursa line (SBL) were significantly more efficient antibody producers than birds from the large bursa line (LBL). However, Immunoglobulin G synthesis by SBL birds was significantly lower than in LBL birds.

“…Without the development of congenic lines one cannot conclude that the B haplotypes solely are responsible for the differences in Cy sensitivity. The Cy differences in sensitivity between SB and LJD lines adds to the growing evidence of the difference between the two lines: slower bursal growth and differentiation of the SB line (Glick and Dreesen, 1967;Landreth and Glick, 1973); delayed Ig synthesis by the SB line (Scott et ah, 1988); and a significantly greater IgG antibody production with age in the SB line (Yamamoto and Glick, 1982). Data presented suggest that SDC are resistant to an in ovo dose of 1.5% Cy.…”

Section: Discussionmentioning

confidence: 62%

The Effect of Cyclophosphamide on Bursal and Splenic Dendritic Cells ,

Marsh

Glick

1992

The effect of in ovo and posthatch administration of cyclophosphamide, an immunosuppressive drug, on avian dendritic cells was examined in New Hampshire chickens. The Small Bursa line of New Hampshire chickens was more sensitive to the toxic effects of this drug than the line from which it was selected, the Lester J. Dreesen line. The bursal secretory dendritic cells, which may effect B cell differentiations, were left intact and appeared more prominent after treatment. The ellipsoid-associated cells, the dendritic cells of the spleen that bind and carry antigen, became vacuolated after treatment, but normal ellipsoid-associated cells were found within 1 wk. Ellipsoid-associated cells were able to bind and move carbon away from the ellipsoid areas of the spleen 5 wk after in ovo treatment with cyclophosphamide. However, the ability of treated birds to mount an antibody response to SRBC was compromised. The present study revealed that bursal dendritic cells were resistant to cyclophosphamide treatment during embryogenesis and the same embryonic treatment did not influence the migrating capability of ellipsoid-associated cells.