Differential Efect of Noradrenaline and Renal Nerve Stimulation on Vascular Resistance in the Dog Kidney and the Release of a Prostaglandin E-Like Substance

Abstract

S U M M A R Y1. The concentrations of prostaglandin E(PGE)-and prostaglandin F(PGF)-like substances in renal venous blood were determined by parallel bioassay of extracts of renal venous effluent before and during adrenergic stimulation of the kidney and were related to simultaneous measurements of renal blood flow and urine flow.2. When noradrenaline was infused continuously into the renal artery, its initial vasoconstrictor and antidiuretic effects diminished on seven of eight occasions in six dogs. Rapid recovery of renal blood flow and urine flow was invariably associated with increasing concentration in renal venous blood of a substance having the physicochemical, chromatographic and biological properties of a prostaglandin of the E series. In the one instance when rapid early recovery of renal blood flow was not observed the concentration of PGE-like substance was not increased.3. In contrast, during renal nerve stimulation early rapid recovery of renal blood flow and urine flow did not occur and the concentration of a PGE-like substance in renal venous blood did not increase. The concentration of a PGF-like substance in renal venous effluent did not increase in response to either stimulus. 4. Since PGE,, unlike PGF2=, is a potent renal vasodilator and diuretic, the intrarenal release of this substance by noradrenaline in concentrations similar to those determined for a PGE-like substance (> 0.50 ng/ml assayed as PGE, equivalents) would account for the changes in renal blood flow and urine flow in these experiments when the renal actions of noradrenaline were attenuated. 5. These results support the proposal that renal prostaglandins function in an intrarenal negative feedback control system which regulates antidiuretic and vasoconstrictor systems.

Effect of glucocorticoids on vascular reactivity to vasoactive hormones in rat isolated kidney: lack of relationship to prostaglandins

“…Both NA and AII stimulate prostaglandin synthesis in several tissues including the kidney (Gilmore et al, 1968;McGiff, et al, 1970;1972;Needleman et al, 1973;Mullane & Moncada, 1980;Shebuski & Aiken, 1980). The ability of prostaglandins, particularly PGE2 and PGI2, to reduce vascular reactivity to vasoconstrictor stimuli (Lonigro et al, 1973;Malik et al, 1980;Susic et al, 1981) and the demonstration that glucocorticoids inhibit prostaglandin synthesis in several intact cell systems and perfused organs (Kantrowitz et al, 1975;Tashjian et al, 1975;Lewis & Piper, 1975;Hong & Levine, 1976;Gryglewski, 1976;Herbaczynska-Cedro & Staszewska-Barczak, 1977;Blackwell et al, 1978;Zusman & Keiser, 1980) presumably by stimulating the synthesis of a phospholipase A2 inhibitor(s) (macrocortin, lipomodulin or renocortin) (Flower & Blackwell, 1979;Hirata et al, 1980;Cloix et al, 1983), has led to the proposal that glucocorticoids enhance vascular reactivity to NA by inhibiting the synthesis of PGE2 and PGI2 (Rascher et al, 1980;Axelrod, 1983).…”

Prostaglandin Release From Cat and Dog Spleen

“…Others include heart (Wennmalm & Stjarme, 1971;Samuelsson & Wennmalm, 1971), kidney (Dunham & Zimmerman, 1970;Davis & Horton, 1972;McGiff, Crowshaw, Terragno, Malik & Lonigro, 1972), adipose tissue (Shaw & Ramwell, 1968), stomach (Bennett, Friedmann & Vane, 1967;Coceani, Pace-Asciak, Volta & Wolfe, 1967), and vas deferens (Hedqvist & von Euler, 1972). Since exogenous prostaglandins modify the responses of various organs to adrenergic nerve stimulation (see review by Brody & Kadowitz, 1974), it is possible that endogenous prostaglandins play a physiological role in the responses of such organs to autonomic control.…”

Release of Prostaglandins From the Rabbit Perfused Kidney: Effects of Vasoconstrictors

“…Renal nerve stimulation, noradrenaline (Dunham & Zimmerman, 1970;McGiff, Crowshaw, Terragno, Malik & Lonigro, 1972) and angiotensin II (McGiff, Crowshaw, Terragno & Lonigro, 1970) increased prostaglandin E-like material in the renal vein of the dog. Blockade of prostaglandin output by indomethacin has recently been described by Davis & Horton (1972) in the rabbit kidney.…”