Differential DNA binding properties of three human homeodomain proteins

Corsetti, Maria Teresa; Briata, Paola; Sanseverino, Lorenza; Daga, Antonio; Airoldi, Irma; Simeone, Antonio; Palmisano, Giulio Lelio; Angelini, Cristiano; Boncinelli, Edoardo; Corte, Giorgio

doi:10.1093/nar/20.17.4465

Cited by 48 publications

(33 citation statements)

References 35 publications

(31 reference statements)

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“…5. Unsaturated fatty acids bind with a K d in the order of 2 ϫ 10 Ϫ7 M, while stearic acid binds with a K d of 5 ϫ 10 Ϫ7 M and myristic acid with a K d of 1 ϫ 10 Ϫ6 M. Calculation of the equilibrium binding constants was performed with the following equilibrium equation from the midpoints of the curves (45).…”

Section: Dissociation Constant Determination Of the Fatty Acid-ch21mentioning

confidence: 99%

The Developmentally Regulated Avian Ch21 Lipocalin Is an Extracellular Fatty Acid-binding Protein

Cancedda

Malpeli

Gentili

et al. 1996

Journal of Biological Chemistry

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Ch21, a developmentally regulated extracellular protein expressed in chick embryos and in cultured chondrocytes, was expressed in the baculovirus system, and the recombinant protein was purified to homogeneity by gel-filtration chromatography. Separation of two isoforms was achieved on an ion-exchange column. Previous work had shown that Ch21 belongs to the superfamily of lipocalins, which are transport proteins for small hydrophobic molecules. Studies were performed to identify the Ch21 ligand.By analysis of recombinant Ch21 on native polyacrylamide gel electrophoresis and by Lipidex assay, the binding of fatty acid to the protein was shown and a preferential binding of long-chain unsaturated fatty acids was observed. Both isoforms had the same behavior. Ch21, also present in chicken serum, represents the first extracellular protein able to selectively bind and transport fatty acid in extracellular fluids and serum. We propose to rename the Ch21 protein as extracellular fatty acid-binding protein (Ex-FABP).In the last several years, our laboratory has focused on the cellular and molecular mechanisms that control endochondral bone formation. The process is characterized by the differentiation of prechondrogenic mesenchymal cells to resting, proliferating, and hypertrophic chondrocytes (1-8) and then by the replacement of hypertrophic cartilage by bone, i.e. cartilage calcification, erosion, invasion by blood vessels, and onset of osteogenesis. Hypertrophic chondrocytes play a critical role in the last part of the process. In fact they can undergo further differentiation to osteoblast-like cells both in vitro (9) and, in selected bone regions, in vivo (10 -14) and are essential to the deposition of the first bone on newly synthesized bone sialoprotein-rich matrix ("bone priming").

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Section: Dissociation Constant Determination Of the Fatty Acid-ch21mentioning

confidence: 99%

The Developmentally Regulated Avian Ch21 Lipocalin Is an Extracellular Fatty Acid-binding Protein

Cancedda

Malpeli

Gentili

et al. 1996

Journal of Biological Chemistry

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“…The HOXB7 protein has also been implicated in hematopoietic dierentiation as well as in lymphoid development (Shen et al, 1989;Deguchi et al, 1991;Lill et al, 1995;Magli, 1998). The HOXB7 protein can bind DNA (Corsetti et al, 1992) and activate transcription from distinct promoters in a variety of cell lines (Care et al, 1996;Chariot et al, 1998;Sanlioglu et al, 1998). However, the functional domains that mediate HOXB7 transcriptional properties as well as its interacting partners remain to be identi®ed.…”

Section: Introductionmentioning

confidence: 99%

CBP and histone deacetylase inhibition enhance the transactivation potential of the HOXB7 homeodomain-containing protein

Chariot

Lint²,

Chapelier

et al. 1999

Oncogene

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Homeodomain-containing proteins are transcription factors regulating the coordinated expression of multiple target genes involved in development, dierentiation and cellular transformation. In this study, we demonstrated that HOXB7, one member of this family, behaved as a transactivator in breast cancer cells. Deletion of either the HOXB7 N-terminal domain or the C-terminal acidic tail abolished this transcriptional eect, suggesting a combination of distinct functional transactivating domains. HOXB7 physically interacted both in vitro and in vivo with the coactivator CREB-binding protein (CBP). This interaction led to an enhanced transactivating potential and required the N-terminal of HOXB7 as well as two domains located at the C-terminal part of CBP. Moreover, trichostatin A, a deacetylase inhibitor, strongly enhanced the transcriptional properties of HOXB7. Our data therefore indicate that HOX proteins can directly interact with CBP and that acetylation/ deacetylation may regulate their transcriptional properties.

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“…Each homeodomain that is encoded by a homeobox can bind specifically to a region of double-stranded (ds) DNA with a specific recognition sequence [2][3][4][5][6]. The interactions between homeodomains and DNA have been extensively analyzed by foot-printing methods and gel shift assays [6][7][8][9][10][11][12][13][14]. Direct structural analysis of the complexes has also been performed with NMR and X-ray [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

Seimiya¹,

Kurosawa²

1996

FEBS Letters

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The kinetics of binding of the Antp homeodomain to three kinds of DNA fragment were analyzed by measurements of surface plasmon resonance at various temperatures. Non-specific and specific binding of the homeodomain to DNA was examined. In the case of non-specific binding, the association rate constant (kass) was estimated to be 1.41-2.62X 105 M -1 s -1 and the dissociation rate constant (kdiss) was 1.36-3.10 X 10 -2 s -1, thus, the dissociation constant (KI)) was 0.847-1.72 × 10 7 M. The association seemed to be driven by entropy. In the case of specific binding, by contrast, the enthalpy term seemed to contribute more to the binding than did the entropy term. The kass was 2.04-2.59X105 M -1 s -1 and the kdiss was 0.759-1.16X10 -3 s -1, thus, the /to was 2.93-5.69X 10 -9 M. These values were measured under the condition of 150 mM NaCl. Both interactions were strongly dependent on the concentration of NaC1. The /to at 50 mM NaCI became several tens of times smaller than those at 150 mM. Possible reasons for the differences between non-specific and specific interactions are discussed.

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Differential DNA binding properties of three human homeodomain proteins

Cited by 48 publications

References 35 publications

The Developmentally Regulated Avian Ch21 Lipocalin Is an Extracellular Fatty Acid-binding Protein

The Developmentally Regulated Avian Ch21 Lipocalin Is an Extracellular Fatty Acid-binding Protein

CBP and histone deacetylase inhibition enhance the transactivation potential of the HOXB7 homeodomain-containing protein

Kinetics of binding of Antp homeodomain to DNA analyzed by measurements of surface plasmon resonance

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