Differential diagnosis of sarcomatoid mesothelioma from true sarcoma and sarcomatoid carcinoma using immunohistochemistry

Kushitani, Kei; Takeshima, Yukio; Amatya, Vishwa Jeet; Furonaka, Osamu; Sakatani, Akio; Inai, Kei

doi:10.1111/j.1440-1827.2007.02193.x

Cited by 62 publications

(61 citation statements)

References 27 publications

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“…Thus, EM-D were epithelioid mesothelioma showing papillotubular pattern, micropapillary pattern, and/or microcystic pattern and EM-LD showing solid nest, trabecular pattern, signet-ring cell-like appearance and/or single cell infiltration pattern. One representative tissue block was used for immunohistochemical confirmation of mesothelioma using positive and negative antibody panels mentioned in our previous publications (21)(22)(23)(24)(25). This study consisted of 81 cases of epithelioid mesothelioma (EM) (61%), 34 cases of sarcomatoid mesothelioma (SM) (25%) and 19 cases of biphasic mesothelioma (BM) (14%).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of CD26/DPPIV in mesothelioma tissue and mesothelioma cell lines

et al. 2011

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Abstract. Mesothelioma, a highly aggressive cancer with poor prognosis and refractory to currently available therapies show increasing trends of its incidence in Japan and other developing countries. Although surgery is a gold standard for patients with early mesothelioma, most patients with advanced disease are not suitable for surgical resection and have option of palliative chemotherapy alone. One of the new treatment strategies for mesothelioma, the humanized anti-CD26 monoclonal antibody therapy is under development. CD26, a 110-kDa transmembrane glycoprotein with known dipeptidyl peptidase IV activity, plays a role in tumor development and its expression was reported in various human malignancies. This study determined the preliminary selection criteria for humanized monoclonal anti-CD26 antibody therapy. Eighty-one epithelioid (49 differentiated and 32 less differentiated), 34 sarcomatoid, 19 biphasic mesothelioma and 8 mesothelioma cell lines were immunohistochemically examined using 8 different commercially available anti-CD26 antibodies for membranous and cytoplasmic expression. The cytoplasmic expression of CD26 was observed in all histological types of mesothelioma, while the membranous expression of CD26 was found in 88% of differentiated and 69% of less differentiated epithelioid mesothelioma, and none of sarcomatoid mesothelioma with anti-CD26 antibodies with rabbit polyclonal anti-DPP4 antibody and similar results were also obtained with goat polyclonal anti-DPP4/CD26 antibody. These antibodies absorbed with soluble human CD26 proteins do not show CD26 expression in mesothelioma tissue, suggesting these two antibodies localize true CD26 protein. Seven mesothelioma cell lines, including sarcomatoid types, also showed membranous expression of CD26 in cellblock preparation.CD26 vector transfection to CD26-negative MSTO-211H cells showed membranous expression of CD26 by flow cytometry, but not in tumor developed in NOD/SCID mice with inoculation of CD26 vector transfected MSTO-211H cells. We found that both rabbit and goat polyclonal antibodies are suitable for immunohistochemical evaluation of membranous expression of CD26 in mesothelioma.

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Section: Methodsmentioning

confidence: 99%

Overexpression of CD26/DPPIV in mesothelioma tissue and mesothelioma cell lines

et al. 2011

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“…Additionally, while desmin and smooth muscle actin are not considered discriminatory markers for sarcomatous mesothelioma, their expression pattern as reported herein has been described in human sarcomatous mesotheliomas. 9 The pericardial, pleural, and peritoneal cavities, internal organs, and tunica vaginalis of the testes are lined by a monolayer of cells termed the mesothelium. The mesothelium functions to provide protection from physical damage and invading pathogens.…”

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confidence: 99%

Peritoneal fibrosarcomatous mesothelioma in a cat

et al. 2011

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A 4.5-kg female spayed Domestic Shorthair cat presented to the Iowa State University Emergency Service (Ames, Iowa) with a 1-day history of anorexia, adipsia, weakness, and lethargy. The patient was not current on rabies or feline viral rhinotracheitis, calicivirus, and panleukopenia vaccinations and was housed outdoor and indoor. The owners noted the cat was severely ataxic the evening prior to presentation, and that she had vomited the morning of presentation.On presentation, the patient was semicomatose. Physical examination revealed severe hypothermia (34.3°C), bradycardia (137 beats/min), and eupnea (16 breaths/min). She was 10-12% dehydrated and severely hypotensive, with a systolic blood pressure of 50 mmHg and weak, thready femoral pulses. The abdomen was severely distended and symmetrically enlarged.An abdominocentesis was performed, and 1,030 ml of serosanguineous fluid was removed. Fluid analysis revealed glucose of 307 mg/dl, lactate of 9.1 mmol/l, and specific gravity of 1. Abstract. Primary tumors of serosal surfaces are uncommon in veterinary medicine. Mesothelial neoplasms can be benign or malignant, and are classified as predominantly epitheloid, mixed (biphasic), or fibrous (spindle cell, fibrosarcomatous), with fibrous mesotheliomas reported least in domestic species. A 9-year-old Domestic Shorthair cat presented on emergency with a brief history of weakness and lethargy. On presentation, the cat was semicomatose, hypothermic, and hypotensive with a markedly distended abdomen. Approximately 1 liter of serosanguineous fluid was removed via abdominocentesis. Diagnostic imaging and cytologic evaluation of fine-needle aspirates were suggestive of neoplasia, and the cat was subsequently euthanized. At necropsy, the omentum was contracted cranially into an irregular lobular mass that surrounded the stomach and proximal intestinal tract, and focally infiltrated the spleen. Both visceral and parietal peritoneal surfaces were thickened and contained off-white friable material and occasionally firm fibrous plaques. Microscopically, serosal surfaces were expanded by neoplastic spindle cells, which were often accompanied by moderate to abundant fibrous stroma. Neoplastic cells had varying degrees of immunoreactivity for cytokeratin, vimentin, desmin, and smooth muscle actin, which was consistent with the diagnosis of mesothelioma.

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“…Definite pathological diagnosis of MM should be based on immunohistochemical reactivity to some markers such as calretinin and thrombomodulin, in addition to the usual hematoxylin-eosin staining (Kushitani et al 2008). Recently, Takeshima et al reported that the diagnosis of MM was suspicious in approximately 15% cases who died of ''MM'' in Japan (Takeshima et al 2009).…”

Section: Discussionmentioning

confidence: 97%