Differential Diagnosis of Respiratory Viruses by Using Real Time Rt-PCR Methodology

Paulino, Renato de Souza; Benega, Margarete Aparecida; Santos, Katia Corrêa de Oliveira; Silva, Daniela Bernardes Borges da; Pereira, Juliana Cristina; Sasaki, Norio Augusto; Silva, Patricia Evelin; Curti, Suely Pires; Oliveira, Maria Isabel de; Carvalhanas, Telma Regina Marques Pinto; Peret, Teresa C. T.; Erdman, Dean D.; Paiva, Terezinha Maria de

doi:10.1590/s0036-46652013000600012

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…Especially in developing countries, RTIs-induced mortality is high. RTIs are mainly caused by viruses, and the most commonly detected viruses among children with RTIs are respiratory syncytial virus (RSV), influenza virus A (FA), influenza virus B (FB), parainfluenza viruses, and adenoviruses [ 5 , 6 ]. Most infections are limited to the upper respiratory tract and only 5% involve the lower respiratory tract [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and Seasonality of Respiratory Viruses Detected from Children with Respiratory Tract Infections in Wuxi, East China

Guo

Chen

et al. 2018

Med Sci Monit

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BackgroundRespiratory tract infections (RTIs) are the major causes of mortality and morbidity in children and lead to hospitalization in developing countries. However, little is known about the epidemiology and seasonality of respiratory viruses in the pediatric population in Wuxi, East China.Material/MethodsWe included all patients 14 years of age and below who presented with signs and symptoms of RTIs between January 2010 and December 2016. During this period, a total of 2160 children treated in Wuxi No. 2 People’s Hospital were involved in our study. The clinical and sociodemographic data were recorded to describe the frequency and seasonality. Respiratory specimens were tested by multiplex real-time PCR assays for virus identification.ResultsMore than 30% (35.19%, 760 samples) of the specimens showed evidence of infection with viruses, including respiratory syncytial virus (368 samples), influenza virus A (114 samples), influenza virus B (115 samples), parainfluenza virus I (29 samples), parainfluenza virus II (39 samples), parainfluenza virus III (13 samples), and adenovirus (82 samples); 48.99% of the children infected with viruses were under 12 months of age. Viruses were detected throughout all the year, with a peak in winter.ConclusionsOur study found that RSV is the most important cause of RTIs in our region during winter. Our data provide a comprehensive understanding of the epidemiology and seasonality of virus, which may help to reduce the use of antibiotics and implement an effective approach for prevention, control, and treatment of RTIs, especially during its peak season.

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Section: Introductionmentioning

confidence: 99%

Epidemiology and Seasonality of Respiratory Viruses Detected from Children with Respiratory Tract Infections in Wuxi, East China

Guo

Chen

et al. 2018

Med Sci Monit

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“…Enrolled participants complete a questionnaire and nasal swab samples and a serological sample are collected. Nasal swab samples are tested by real time reverse transcriptase polymerase chain reaction (RT-PCR) for several arboviral and respiratory viruses including Influenza (A & B), RSV, AdV, and other respiratory viruses (i.e., human parainfluenza virus types 1 & 3 and human metapneumovirus), as previously described [ 13 , 15 – 17 ].…”

Section: Methodsmentioning

confidence: 99%

Reduced spread of influenza and other respiratory viral infections during the COVID-19 pandemic in southern Puerto Rico

et al. 2022

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Introduction Impacts of COVID-19 mitigation measures on seasonal respiratory viruses is unknown in sub-tropical climates. Methods We compared weekly testing and test-positivity of respiratory infections in the 2019–2020 respiratory season to the 2012–2018 seasons in southern Puerto Rico using Wilcoxon signed rank tests. Results Compared to the average for the 2012–2018 seasons, test-positivity was significantly lower for Influenza A (p<0.001) & B (p<0.001), respiratory syncytial virus (RSV) (p<0.01), respiratory adenovirus (AdV) (p<0.05), and other respiratory viruses (p<0.001) following March 2020 COVID-19 stay at home orders. Conclusions Mitigation measures and behavioral social distancing choices may have reduced respiratory viral spread in southern Puerto Rico.

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“…Blood, urine, and combined nasopharyngeal and oropharyngeal swabs were collected from enrolled patients, and diagnostic testing was performed. Upper respiratory tract specimens were tested for influenza A and B viruses, adenovirus, respiratory syncytial virus (RSV), coronavirus, parainfluenza viruses and human metapneumovirus by real‐time reverse transcription polymerase chain reaction (RT–PCR) as previously described 13‐15 . For each respiratory pathogen, a positive result was defined as nucleic acid detected by PCR (cycle threshold cut–off 37.0).…”

Section: Methodsmentioning

confidence: 99%

Recent influenza activity in tropical Puerto Rico has become synchronized with mainland US

Paz–Bailey

Quandelacy

Adams

et al. 2020

Influenza Resp Viruses

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Background We used data from the Sentinel Enhanced Dengue Surveillance System (SEDSS) to describe influenza trends in southern Puerto Rico during 2012‐2018 and compare them to trends in the United States. Methods Patients with fever onset ≤ 7 days presenting were enrolled. Nasal/oropharyngeal swabs were tested for influenza A and B viruses by PCR. Virologic data were obtained from the US World Health Organization (WHO) Collaborating Laboratories System and the National Respiratory and Enteric Virus Surveillance System (NREVSS). We compared influenza A and B infections identified from SEDSS and WHO/NREVSS laboratories reported by US Department of Health and Human Services (HHS) region using time series decomposition methods, and analysed coherence of climate and influenza trends by region. Results Among 23,124 participants, 9% were positive for influenza A and 5% for influenza B. Influenza A and B viruses were identified year‐round, with no clear seasonal patterns from 2012 to 2015 and peaks in December‐January in 2016‐2017 and 2017‐2018 seasons. Influenza seasons in HHS regions were relatively synchronized in recent years with the seasons in Puerto Rico. We observed high coherence between absolute humidity and influenza A and B virus in HHS regions. In Puerto Rico, coherence was much lower in the early years but increased to similar levels to HHS regions by 2017‐2018. Conclusions Influenza seasons in Puerto Rico have recently become synchronized with seasons in US HHS regions. Current US recommendations are for everyone 6 months and older to receive influenza vaccination by the end of October seem appropriate for Puerto Rico.

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Differential Diagnosis of Respiratory Viruses by Using Real Time Rt-PCR Methodology

Cited by 5 publications

References 4 publications

Epidemiology and Seasonality of Respiratory Viruses Detected from Children with Respiratory Tract Infections in Wuxi, East China

Epidemiology and Seasonality of Respiratory Viruses Detected from Children with Respiratory Tract Infections in Wuxi, East China

Reduced spread of influenza and other respiratory viral infections during the COVID-19 pandemic in southern Puerto Rico

Recent influenza activity in tropical Puerto Rico has become synchronized with mainland US

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