Differential diagnosis of dog hookworms based on PCR-RFLP from the ITS region of their rDNA

Silva, Leandra M. Clara e; Miranda, Rodrigo Rodrigues Cambraia de; Santos, Hudson Andrade dos; Rabelo, Élida Mara Leite

doi:10.1016/j.vetpar.2006.04.012

Cited by 29 publications

(22 citation statements)

References 15 publications

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“…In addition, this region has higher inter-species differences when compared with ITS-1, which ranges from 0.9% to 13.2% among Ancylostoma species. Our ITS-2 sequences (UVAS_1711379_21 and UVAS_1708317_31) showed 100% homology with A. caninum Genbank sequences and clustered with a Brazilian isolate of A. caninum (e Silva et al 2006) (Figure 4). DNA amplicons clustered with most of the retrieved sequences from Genbank and seemed to be clustered within a single clade.…”

Section: Resultsmentioning

confidence: 86%

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Rehman¹,

Akhtar²,

Akbar³

et al. 2017

Vet. Med. - Czech

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ABSTRACT:The molecular prevalence of Ancylostoma caninum was determined in stray and pet dogs in Lahore, Pakistan from July 2014 to August 2015. A total of 500 dog faecal samples were first evaluated using a sedimentation technique and further through a PCR assay targeting the ITS-2 region of the A. caninum genome. Overall, 130 (26%) samples were positive for hookworm infestation by microscopic examination. Only microscopically positive samples were processed for PCR and 89 (17.6%) were positive for A. caninum. Sequence analysis of amplicons showed 100% homology with A. caninum and the genotypes clustered in one clade with Brazilian A. caninum hookworms. There was a significantly (P < 0.05) increased prevalence in male dogs younger than six months old. Labrador retrievers had higher A. caninum incidence compared to German shepherds and other breeds. To the best of our knowledge, this is the first report of the molecular prevalence of A. caninum in dogs in Pakistan. The novel results of the present study allow us to conclude that A. caninum is more prevalent in pet dogs, especially puppies, and this can be a potential threat for humans that come into contact with such animals. Therefore, the routine monitoring of pets, especially the more susceptible breeds, is essential for disease control.

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Section: Resultsmentioning

confidence: 86%

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

Rehman¹,

Akhtar²,

Akbar³

et al. 2017

Vet. Med. - Czech

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“…However, the only reports of A. ceylanicum in Australia (Stewart, 1994;Gasser et al, 1996;Adams, 2004) have been found to be based on a misidentification of A. braziliense as A. ceylanicum (Traub et al, 2006;e Silva et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum

Palmer

Traub

Robertson

et al. 2007

Veterinary Parasitology

103

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There is no current information regarding the prevalence of hookworm in Australian dogs and cats and based on the results of studies conducted over 20 years ago, where high prevalences of helminths were recorded, the prophylactic administration of broad spectrum anthelmintics has been advocated.During this study, faecal samples were collected from dogs (n = 1391) and cats (n = 1027) across Australia. Samples were examined by microscopy and information regarding the demographics of each animal, and the management practices they experienced were recorded. A highly sensitive and species-specific PCR-RFLP technique was utilized to differentiate the various hookworm species which can infect dogs and cats directly from eggs in faeces. The prevalence of hookworm in dogs and cats was found to be 6.9% and 1.4%, respectively. Ancylostoma ceylanicum was detected for the first time in Australia in 10.9% of the dogs found positive for hookworm. Significantly, A. ceylanicum is capable of causing a patent infection in humans. After adjusting for other factors with multiple logistic regression, dogs from refuges, dogs originating from a tropical climatic zone, dogs aged 1 year or less, and those dogs which had not received anthelmintics were significantly more likely to be parasitized. Only univariate analysis was conducted for the cats as there were too few samples positive for hookworm. Cats were more likely to be infected with hookworm if they were from refuges, originated from a tropical climatic zone, and had not received treatment with anthelmintics.The results of this study demonstrates the importance of having current information regarding the prevalence of parasites of dogs and cats and the risk factors associated with infection, as well as the need to reassess the veterinary and public health concerns regarding hookworm infection and its control, which are currently based on out-dated information.

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“…Restriction digestion is as a matter of routine using for the determination of the species identification of closely related parasitic and plant parasitic nematodes. Results indicate the usefulness of these markers for diagnostic purposes, and epidemiologic and molecular-systematic studies on parasites and other eukaryotic organisms (Conole et al 1999;Braasch et al 1999;Nuchprayoon et al 2006;De Silva et al 2006). In conclusion, the populations undergoing arrested development and without ability of undergoing arrested development of T. colubriformis can be distinguished by using PCR amplification of cistron rDNA, its digestion with Hinc II restriction endonuclease, and separation of the restriction products by polyacrylamide gel electrophoresis or agarose gel.…”

Section: Resultsmentioning

confidence: 92%

Trichostrongylus colubriformis rDNA polymorphism associated with arrested development

et al. 2008

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Polymerase chain reaction-restriction fragment length polymorphism) was performed on the cistron of rDNA in the two groups of infective larvae Trichostrongylus colubriformis-the population with and without ability to undergo arrested development. General primers designed by Caenorhabditis elegans rDNA were used for the amplification of the rDNA cistron between genes 18S and 28S. Amplified fragments were digested by using a series of restriction endonucleases. Hinc II restriction profiles unique for each T. colubriformis populations were observed, and therefore enzyme Hinc II appears to be useful for the determination of populations with and without the ability to undergo arrested development. Molecular markers of arrested development ability have not been studied on this part of rDNA before.

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Differential diagnosis of dog hookworms based on PCR-RFLP from the ITS region of their rDNA

Cited by 29 publications

References 15 publications

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets

The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum

Trichostrongylus colubriformis rDNA polymorphism associated with arrested development

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