Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR

“…Newcastle disease virus (NDV) is belong to a group of the avian paramyxovirus -I [7][8][9]. The disease is characterized by an involvement of digestive, respiratory and nervous systems [10][11]. This disease can vary in nature from mild to severe, depending upon the type of the virus.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Ashraf

Shah

Habib

et al. 2016

Braz. arch. biol. technol.

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“…현재 개발된 유전자 진단법의 상당수는 바이러 스 스트레인 간 변동성이 큰 F유전자를 포함한 부위를 표적 부위로 삼고 있다 (Aldous et al, 2001;Antal et al, 2007;Fuller et al, 2009;Kwon et al, 2006;Pham et al, 2005;Tiwari et al, 2004 (Fuller et al, 2010;Mia Kim et al, 2008 …”

Section: 사했을 때unclassified

“…NDV 유전형(genotype)은 F 유전자 염기서열에 기초한 계 통분석법(phylogenetic analysis)에 의해 class I과 calss II로 크게 구분하며, class II는 다시 현재까지 최소한 16개 이상의 유전형(유전형 I 내지 XVI)으로 존재하고 있다 (Diel et al, 2012). Class I은 주로 야생조류와 재래시장의 닭과 오리류 등에서 주로 분리되며, 닭에서 병증을 유발하지 않는다 (Kim et al, 2007;Liu et al, 2007;Zhu et al, 2014 (Aldous et al, 2001;Fuller et al, 2009;Fuller et al, 2010;Kwon et al, 2006;Pham et al, 2005;Tiwari et al, 2004). 특히, NDV 검 출 및 병원성 감별 진단 (Fuller et al, 2009;Kwon et al, 2006), 또는 NDV와 다른 가금바이러스 병원체 감별 진단을 위한 다 양한 RT-PCR 또는 실시간 RT-PCR 검사법들이 개발되고 있 다 Malik et al, 2004;Tang et al, 2012 …”

unclassified

Development of a Duplex RT-PCR Assay for the Simultaneous Detection and Discrimination of Avirulent and Virulent Newcastle Disease Virus (NDV)

Kim¹,

Lee²,

Jang³

et al. 2017

Korean Journal of Poultry Science

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A duplex RT-PCR (dRT-PCR) assay was developed for the simultaneous detection and discrimination of nonvirulent and virulent Newcastle disease virus (NDV) in a single PCR tube. Primers targeting the large polymerase protein (L) gene and the fusion protein (F) gene of NDV were designed to detect all NDVs (by common type PCR primers) and virulent NDVs (by pathotype PCR primers), respectively and evaluated experimentally with reference NDV strains and other poultry viral pathogens. PCR products of the expected size of 386 bp were amplified from all NDV samples whereas PCR products of the expected size of 229 bp were amplified from virulent NDV samples alone. Cross reaction was not observed with other avian viral pathogens. The detection limit of NDV by the dRT-PCR was estimated to be 10 3 50% egg infectious dose/0.1 mL. In the dRT-PCR using field isolates of NDV, the pathotype PCR primers detected specifically all of virulent field isolates of NDV from Malaysia, Pakistan and China whereas common type PCR primers detected 94.4% (51/54) of field isolates of NDV from China. Three Chinese NDV isolates with false negative result were non-virulent viruses. Our results indicate that the dRT-PCR might provide a rapid and simple tool for rapid simultaneous detection and discrimination of non-virulent and virulent NDVs. Therefore the developed dRT-PCR assay provides a powerful novel means for the rapid diagnosis of Newcastle disease.

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“…These tests are cumbersome, labor-intensive, inhumane and, sometimes, inconclusive (Tiwari et al, 2004). Virological, pathologic and immunohistochemical analysis of chickens with ND in field conditions have been useful in the investigation of the mechanisms of ND outbreaks in vaccinated chickens (Nakamura et al, 2008;Bwala et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Use of immunohistochemistry (IHC) in the detection of Newcastle disease virus (NDV) in experimentally and naturally infected birds

Carrasco¹,

Seki²,

Mineo³

et al. 2015

Afr. J. Microbiol. Res.

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The objective of the present study was to standardize an immunohistochemical reaction (IHC) for the diagnosis of Newcastle disease virus (NDV) in tissue samples. In order to achieve this aim, pigeons (Columba livia) and chicken (Gallus gallus) were experimentally infected with a pathogenic sample of NDV (São Joao do Meriti strain). The IHC reaction was standardized and tested in samples of lung, liver, spleen and trachea of the experimentally infected birds, and the results showed that from the 20 infected birds, 17 were positive for Newcastle disease antigen immunomarking in the trachea and 11 were positive in the spleen. NDV detection was not achieved in samples of lung and liver, and these tissues are not indicated in NDV detection in the present study.After that, the standardized technique was used in the detection of NDV in samples of trachea and spleen of 46 free-living birds which belonged to 11 different bird orders. The virus was detected in 24 individuals. The order Caprimulgiforme was the only one that did not show any positive animal. These NDV strains circulate in bird populations, generally without causing the disease, in a parasite vs. host balance. Outbreaks may occur when these free-living birds get in contact with commercial birds, with considerable losses to countries that raise and export poultry and poultry products. In conclusion, IHC is a diagnostic tool that may be used in locations that do not have biosafety levels for virus isolation, or that are even not able to perform molecular assays.

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Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR

Cited by 26 publications

References 12 publications

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Development of a Duplex RT-PCR Assay for the Simultaneous Detection and Discrimination of Avirulent and Virulent Newcastle Disease Virus (NDV)

Use of immunohistochemistry (IHC) in the detection of Newcastle disease virus (NDV) in experimentally and naturally infected birds

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