Differential decreases in c-fos and aldolase C mRNA expression in the rat cerebellum after repeated administration of methamphetamine

Hamamura, Mitsuko; Ozawa, Hidetoshi; Kimuro, Yoshihiko; Okouchi, Jiro; Higasa, Koichiro; Iwaki, Akiko; Fukumaki, Yasuyuki

doi:10.1016/s0169-328x(98)00306-4

Cited by 18 publications

(9 citation statements)

References 46 publications

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“…Namima and co-investigators (1999) reported that chronic treatment of mice with METH (2 mg/kg) given five times at intervals of 3 days attenuated the acute effects of the drug on c-Fos protein expression in the striatum. Hamamura and others (1999) also found that daily injections of METH (5 mg/kg) for 3 days also attenuated the acute effects of METH on c-fos mRNA levels in the cerebellum. Therefore, more experiments were necessary to identify the effects of chronic METH exposure on IEG expression in the striatum.…”

Section: Introductionmentioning

confidence: 73%

Chronic methamphetamine exposure suppresses the striatal expression of members of multiple families of immediate early genes (IEGs) in the rat: normalization by an acute methamphetamine injection

et al. 2011

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Rationale Repeated injections of cocaine cause blunted responses to acute cocaine challenge-induced increases in the expression of immediate early genes (IEGs). Objectives The aim of this study was to test if chronic methamphetamine (METH) exposure might cause similar blunting of acute METH-induced increases in IEG expression. Results Repeated saline or METH injections were given to rats over 14 days. After one day of withdrawal, they received a single injection of saline or METH (5 mg/kg). Acute injection of METH increased c-fos, fosB, fra2, junB, Egr1-3, Nr4a1 (Nur77), and Nr4a3 (Nor-1) mRNA levels in the striatum of saline-pretreated rats. Chronic METH treatment alone reduced the expression of AP1, Erg1-3, and Nr4a1 transcription factors below control levels. Acute METH challenge normalized these values in METH-pretreated rats. Unexpectedly, acute METH challenge to METH-pretreated animals caused further decreases in Nr4a2 (Nurr1) mRNA levels. In contrast, the METH challenge caused significant but blunted increases in Nr4a3 and Arc expression in METH-pretreated rats. There were also chronic METH-associated decreases in the expression of cAMP responsive element binding protein (CREB) which modulates IEG expression via activation of the cAMP/PKA/CREB signal transduction pathway. Chronic METH exposure also caused significant decreases in preprotachykinin, but not in prodynorphin, mRNA levels. Conclusions These results support the accumulated evidence that chronic administration of psychostimulants is associated with blunting of their acute stimulatory effects on IEG expression. The METH-induced renormalization of the expression of several IEGs in rats chronically exposed to METH hints to a potential molecular explanation for the recurrent self-administration of the drug by human addicts.

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Section: Introductionmentioning

confidence: 73%

Chronic methamphetamine exposure suppresses the striatal expression of members of multiple families of immediate early genes (IEGs) in the rat: normalization by an acute methamphetamine injection

et al. 2011

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“…These studies analyzed the effects of METH on gene expression or electrophysiology of cerebellar neurons. In particular, within the Purkinje cells METH induces the early gene c‐fos , 12 the corticosterone receptor, 13 and the aldolase C 12 …”

Section: Discussionmentioning

confidence: 99%

Effects of Methamphetamine on the Cerebellar Cortex

Ferrucci¹,

Busceti²,

Falleni³

et al. 2006

Annals of the New York Academy of Sciences

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Methamphetamine (METH) targets monoamine nerve terminals and produces motor effects, which are related to changes in catecholamine activity within the basal ganglia. Cerebellum plays an important role in motor control, nonetheless only a few studies investigated the effects of METH in this area. In this article, we report preliminary results on protein expression in the cerebellum following METH administration. In particular, we focused on the rate-limiting catecholamine-synthesizing enzyme tyrosine hydroxylase (TH). By using immunoblotting, we found that METH administration produces a dose-dependent increase of TH within the cerebellar cortex of mice, which is opposite to the decrease of TH within the striatum. Further investigations are needed in order to determine the time course, the cerebellar regions, the cellular (and subcellular) compartments, and the functional role related to these effects.

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“…Reduction in neural activation has previously been reported following repeated exposure to psychostimulants (e.g., cocaine, amphetamine, MA). In general, these studies have primarily focused on examining alterations in immediate early gene responses within the nucleus accumbens, caudate putamen, cerebellum, and cerebral cortex with few studies examining HPA axis-associated regions (McCoy et al 2011; Hamamura et al 1999). In one study, Zahm et al (2010) reported that self-administration of cocaine increased c-Fos immunoreactivity in the extended amygdala on the 6th day of exposure compared to the 1st day.…”

Section: Discussionmentioning

confidence: 99%

Immediate and lasting effects of chronic daily methamphetamine exposure on activation of cells in hypothalamic-pituitary-adrenal axis-associated brain regions

et al. 2015

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Rationale Chronic methamphetamine (MA) abuse leads to dependence and symptoms of withdrawal after use has ceased. Negative mood states associated with withdrawal, as well as drug reinstatement, have been linked to drug-induced disruption of the hypothalamic-pituitary-adrenal (HPA) axis. However, effects of chronic MA exposure or acute MA exposure following withdrawal on neural activation patterns within brain regions that regulate the HPA axis are unknown. Objectives In this study, neural activation patterns were assessed by quantification of c-Fos protein in mice exposed to different regimens of MA administration. Methods (Experiment 1) Adult male mice were treated with MA (5 mg/kg) or saline once or once daily for 10 days. (Experiment 2) Mice were treated with MA or saline once daily for 10 days and following a 10-day withdrawal period were re-administered a final dose of MA or saline. c-Fos was quantified in brains after the final injection. Results (Experiment 1) Compared to exposure to a single dose of MA (5 mg/kg), chronic MA exposure decreased the number of c-Fos expressing cells in the paraventricular hypothalamus, dorsomedial hypothalamus, central amygdala, basolateral amygdala, bed nucleus of the stria terminalis (BNST), and CA3 hippocampal region. (Experiment 2) Compared to mice receiving their first dose of MA, mice chronically treated with MA, withdrawn, and re-administered MA, showed decreased c-Fos expressing cells within the central and basolateral amygdala, BNST, and CA3. Conclusions HPA axis-associated amygdala, extended amygdala, and hippocampal regions endure lasting effects following chronic MA exposure and therefore may be linked to stress-related withdrawal symptoms.

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Differential decreases in c-fos and aldolase C mRNA expression in the rat cerebellum after repeated administration of methamphetamine

Cited by 18 publications

References 46 publications

Chronic methamphetamine exposure suppresses the striatal expression of members of multiple families of immediate early genes (IEGs) in the rat: normalization by an acute methamphetamine injection

Chronic methamphetamine exposure suppresses the striatal expression of members of multiple families of immediate early genes (IEGs) in the rat: normalization by an acute methamphetamine injection

Effects of Methamphetamine on the Cerebellar Cortex

Immediate and lasting effects of chronic daily methamphetamine exposure on activation of cells in hypothalamic-pituitary-adrenal axis-associated brain regions

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