Differential Damage in Bacterial Cells by Microwave Radiation on the Basis of Cell Wall Structure

Woo, Im-Sun; Rhee, In‐Koo; Park, Heui-Dong

doi:10.1128/aem.66.5.2243-2247.2000

Cited by 235 publications

(197 citation statements)

References 33 publications

Supporting

Mentioning

178

Contrasting

Unclassified

Order By: Relevance

“…Meanwhile, there has been research demonstrating additional effects, beyond the purely thermal mode of inactivation. For example, distortion of membrane structure and function (Phelan et al, 1994), altered enzyme activity (Dreyfuss and Chipley, 1980), disruption of weak bonds (Betti et al, 2004), increased release of various substances (Woo et al, 2000;Celandroni et al, 2004;Campanha et al, 2007), and increased ionic strength due to an increased current within cells (Watanabe et al, 2000) have all been reported. However, all of the aforementioned research was conducted in the liquid, solid, or aqueous phase.…”

Section: Introductionmentioning

confidence: 99%

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Woo

Grippin

et al. 2012

Aerosol Air Qual. Res.

View full text Add to dashboard Cite

Inactivation of collected viral aerosols is important for preventing a filter medium's serving as a fomite. The focus of this study was to evaluate the inactivation efficiency (IE) achieved through filtration coupled with microwave irradiation. MS2 aerosolized through a Collison nebulizer was fed into the system and collected onto the filter. For in-flight microwave decontamination, microwave irradiation was applied to an HVAC (heating, ventilation and air conditioning) filter supported on a SiC disk for three cycles of selected irradiation times per 10 min (i.e., 1, 2.5, 5, and 10 min/10 min) at power levels ranging from 125 W to 375 W. The survival fraction (SF) on the substrate and the IE through the entire system were investigated to determine the efficacy of this approach. SF decreased and IE increased as microwave power level was increased (p = 0.02 and p < 0.01, respectively) or the application time was extended (p = 0.03 and p < 0.01, respectively). Both measures changed sharply above a threshold temperature of around 90°C and reached 2 logs at 116 and 109°C, respectively. The log SF and IE of -2.59 and 3.62, respectively, were observed when the operating condition of 375 W for 10 min/cycle was used and the SiC disk facilitated microwave absorption. When a quartz frit was used as a support instead of the SiC disk, log inactivation efficiencies of 0.8, 1.0, and 1.3 were measured at relative humidities of 30%, 60% and 90%, respectively, under the same irradiation conditions. Relative humidity is a significant parameter from 50-80°C (p = 0.01). The results demonstrate that microwave-assisted filtration systems can be used as an effective means for inactivating viruses.

show abstract

Section: Introductionmentioning

confidence: 99%

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Woo

Grippin

et al. 2012

Aerosol Air Qual. Res.

View full text Add to dashboard Cite

show abstract

“…Similar results were reported in literature after different chemical treatments were applied. Microwave irradiation on temperatures above 60˚C reduces the number of viable cells on E. coli [26]. Thermal treatments onto different cell suspensions of Aspergillus carbonarius for 2 h at 50˚C led to an important decrease of Cu 2+ uptake [27] or Cr(VI) accumulation by Neurospora crassa [28].…”

Section: Effect Of Biomass Treatment On Cadmium Ions Uptake At Room Tmentioning

confidence: 99%

Biosorption of Cadmium Ions by Unmodified, Microwave and Ultrasound Modified Brewery and Pure Strain Yeast Biomass

Nagy¹,

Tonk²,

Indolean³

et al. 2013

AJAC

View full text Add to dashboard Cite

The present study investigates the biosorption of cadmium ions from aqueous solution onto unmodified, ultrasound and microwave treated cells of Saccharomyces cerevisiae. FTIR analysis was conducted to characterize the biosorbent. Equilibrium and kinetic studies of unmodified RBW (residual brewery waste) cell yeast and DSM 1333 (pure strain) were conducted by considering the effect of initial cadmium ions concentration. Results showed that the ultrasound and microwave treatments decrease the heavy metal uptake compared with the unmodified biomass. Langmuir and Freundlich isotherm models on both unmodified yeast cell types were used to analyze the equilibrium data. It was found that the Langmuir isotherm and pseudo-second-order kinetics models describe better the cadmium ions removal process.

show abstract

“…25 This analysis allowed for the microwave power distribution at that particular position to be uniform, thereby eliminating hot spot effects. 17 Further, because the wavelength of microwaves in the cavity was much greater than the dimensions of the sample (10Â10Â0.5 mm), the possibility of noneven heating due to a nonuniform field distribution was negligible.…”

Section: Treatment Of Inoculated Sample With Microwave Radiationmentioning

confidence: 99%

“…Much research has led to the conclusion that microwave radiation extends tissue preservation by reducing microbial levels. [8][9][10][11][12][13][14][15][16][17] Microwave treatment is known to inactivate many microorganisms, such as Burkholderia cepacia, Clostridium perfringens, E. coli, Photobacterium leiognathii, Streptococcus faecalis, S. aureus, Salmonella, and Listeria spp. [10][11][12][13]15,[17][18][19][20][21][22][23] Bacterial and mould spores have also been reported to be sensitive to microwave radiation.…”

Section: Introductionmentioning

confidence: 99%

A New Sterilization Technique of Bovine Pericardial Biomaterial Using Microwave Radiation

Shamis

Patel²,

Taube³

et al. 2009

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Ivanova, E. (2009). A new sterilization technique of bovine pericardial biomaterial using microwave radiation. Tissue Engineering Part C methods, 15 (3), 445-454. A new sterilization technique of bovine pericardial biomaterial using microwave radiation AbstractBioprosthetic valves created from chemically treated natural tissues such as bovine pericardial biomaterial are used as heart valve scaffolds. Methods currently available for sterilization of biomaterial for transplantation include the application of gamma radiation and chemical sterilants. These techniques, however, can be problematic because they can be expensive and lead to a reduction in tissue integrity. Therefore, improved techniques are needed that are cost effective and do not disrupt the physical properties, functionality, and lifespan of the valvular leaflets. This study examined a novel technique using nonthermalmicrowave radiation that could lead to the inactivation of bacteria in bovine pericardial biomaterial without compromising valve durability. Two common pathogenic species of bacteria, Escherichia coli and Staphylococcus aureus, were used as test microorganisms. Optimized microwave parameters were used to determine whether inactivation of pathogenic bacteria from bovine pericardium could be achieved. In addition, the effect of microwave sterilization on tissue integrity was examined. The mechanical properties (assessed using dynamic mechanical analysis) and tensile strength testing (using a Universal Tensile Tester) as well as thermal analysis (using thermogravimetric analysis and differential scanning calorimetry) indicated that microwave sterilization did not compromise the functionality of bovine pericardial biomaterial. Scanning electron microscopy imaging and cytotoxicity testing also confirmed that the structure and biocompatibility of transplant biomaterial remained unaltered after the sterilization process. Results from the application of this newmicrowave (MW) sterilization technique to bovine pericardium showed that nearcomplete inactivation of the contaminant bacteria was achieved. It is concluded that nonthermal inactivation of pathogenic bacteria from bovine pericardial biomaterial could be achieved using microwave radiation. Disciplines

show abstract

Differential Damage in Bacterial Cells by Microwave Radiation on the Basis of Cell Wall Structure

Cited by 235 publications

References 33 publications

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Microwave-irradiation-assisted HVAC Filtration for Inactivation of Viral Aerosols

Biosorption of Cadmium Ions by Unmodified, Microwave and Ultrasound Modified Brewery and Pure Strain Yeast Biomass

A New Sterilization Technique of Bovine Pericardial Biomaterial Using Microwave Radiation

Contact Info

Product

Resources

About