Differential cytology of bronchoalveolar lavage fluid in normal children

Ratjen, Félix; Bredendiek, Martin; M, Brendel; Meltzer, Jay I.; Costabel, Ulrich

doi:10.1183/09031936.94.07101865

Cited by 122 publications

(129 citation statements)

References 18 publications

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“…The other possibility is to inject cells into a flow cytometre. The results of both technique yield similar results [19,33,37,38,39]. If cytospins are used, a minimum of 300±500 cells should be counted [35].…”

Section: Cellular Componentsmentioning

confidence: 63%

“…To improve comparison between centres the total volume instilled, the number of specimens, their volume and the percentage of recovery should be reported. Total cell counts are variable even in healthy children but should be included [19,33,34]. The percentage of individual cell population is the preferable method for data analysis.…”

Section: Reporting Resultsmentioning

confidence: 99%

“…The first BAL sample has a lower cellular yield and contains more neutrophils and less lymphocytes than the following samples in both healthy children and those with lung diseases [15,33,34]. Subsequent samples can be pooled for cellular and noncellular studies.…”

Section: Processing Of the First Samplementioning

confidence: 99%

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Bronchoalveolar lavage in children

Blic¹,

Midulla²,

Barbato³

et al. 2000

eur respir j

350

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Bronchoalveolar lavage (BAL) is routinely performed in adults for sampling cellular and biochemical components. Many studies have been performed that have enabled the proposal of recommendations for BAL, methodology and processing, as well as reference values in different clinical settings. In contrast, despite a world-wide increase in the use of BAL in children, including neonates, there are, at the present time, no clear recommendations on the methodology, the clinical applications and the research areas for BAL. A Task Force on BAL in children has been approved by the European Respiratory Society (ERS) Task Force committee 1996. The objectives of this task force were: 1) to provide recommendations and guidelines on how to perform BAL and how to process the return fluid in children; 2) to collect and discuss available reference values; 3) to list indications and results in both immunocompetent and immunocompromised children; 4) to define areas for future research.This task force included paediatric respiratory physicians involved in bronchoscopy and BAL and was run as a collaboration between the Paediatric Bronchology Group and the BAL scientific group of the ERS.

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Section: Cellular Componentsmentioning

confidence: 63%

Section: Reporting Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bronchoalveolar lavage in children

Blic¹,

Midulla²,

Barbato³

et al. 2000

eur respir j

350

View full text Add to dashboard Cite

show abstract

“…Children have higher percentages of lymphocytes in BALF than adult humans, which may reflect infection with respiratory viruses (Ratjen et al, 1994). Acute respiratory viral infections also occur with high frequency in young dogs, especially those that are unvaccinated (Buonavoglia and Martella, 2007).…”

Section: Discussionmentioning

confidence: 99%

Influence of age on bronchoscopic findings in healthy beagle dogs

Mercier

Bolognin

Hoffmann

et al. 2011

The Veterinary Journal

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a b s t r a c tThe aims of this study were to evaluate the effect of age on bronchoscopic features and bronchoalveolar lavage fluid (BALF) cellularity in dogs. Thirty healthy beagle dogs from three age groups were included: young dogs (10 months to 4.5 years of age; n = 8), middle-aged dogs (5-8 years old; n = 13) and older dogs (>8 years; n = 9). Haematology, thoracic radiography, bronchoscopy and bronchoalveolar lavage were performed; bronchoscopic findings were scored and BALF total and differential cell counts were determined. The total bronchoscopic score was higher in older dogs; these dogs had more irregular bronchial mucosa, more prominent mucosal vessels and bronchiectasis. Younger dogs had a higher percentage of neutrophils in BALF compared with middle-aged and old dogs and a higher percentage of lymphocytes in BALF compared with middle-aged dogs. The results show that age has an effect on bronchoscopic features of airways and the composition of BALF in the dog.

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“…kg body weight -1 aliquots of normal saline warmed to body temperature [23]. The pooled samples were analysed for differential cell counts and neutrophil surface marker expression as described above.…”

Section: Bronchoalveolar Groupmentioning

confidence: 99%

Effect of 3 weeks' rehabilitation on neutrophil surface antigens and lung function in cystic fibrosis

Nikolaizik¹,

Simon²,

Iseli³

et al. 2000

Eur Respir J

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Neutrophil leukocytes have been shown to be the predominant cells in inflammatory airway infiltrates of cystic fibrosis (CF) patients. The aim of this study was to investigate the effect of rehabilitation on neutrophil surface antigen expression and lung function in healthy controls and stable CF patients with moderately severe disease. The absolute number of neutrophils and the level of surface marker expression on neutrophils were elevated in 12 CF patients compared with eight healthy controls. The level of neutrophil surface marker expression was similar in bronchoalveolar lavage fluid from CF patients who underwent bronchoscopy for diagnostic or therapeutic reasons. After 3 weeks′ rehabilitation, there was a significant reduction in the expression of CD11b (complement receptor type 3), CD13 (aminopeptidase N), CD32 (low‐affinity Fc γ chain receptor II), and CD35 (complement receptor type 1) in only the CF patients. At the same time, lung function improved significantly. The increase in forced vital capacity correlated significantly with the decrease in CD32 level. These results demonstrate that rehabilitation in a specialized clinic can reduce the neutrophil‐dominated inflammation and improve the lung function of stable CF patients with moderately severe disease even without changing any medications.

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Differential cytology of bronchoalveolar lavage fluid in normal children

Cited by 122 publications

References 18 publications

Bronchoalveolar lavage in children

Bronchoalveolar lavage in children

Influence of age on bronchoscopic findings in healthy beagle dogs

Effect of 3 weeks' rehabilitation on neutrophil surface antigens and lung function in cystic fibrosis

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