Differential count and time-course analysis of the cellular composition of coelomocyte aggregate of the Japanese sea cucumber Apostichopus japonicus

Taguchi, Meiko; Tsutsui, Shigeyuki; Nakamura, Osamu

doi:10.1016/j.fsi.2016.06.060

Cited by 21 publications

(17 citation statements)

References 23 publications

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“…Meanwhile, using May-Grunwald/Giemsa staining, Taguchi et al (17) tentatively classified coelomocytes of the sea cucumber Apostichopus japonicus into up to twelve types, including several granulocytes (17). In the starfish Asterias rubens, Gorshkov et al (18) detected just two types of cells and termed them "mature" and "immature" coelomocytes.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Smith et al ( 7 ) identified only three categories of coelomocytes in sea urchins (phagocytes, spherule cells or amoebocytes, and vibratile cells). Meanwhile, using May-Grunwald/Giemsa staining, Taguchi et al ( 17 ) tentatively classified coelomocytes of the sea cucumber Apostichopus japonicus into up to twelve types, including several granulocytes ( 17 ). In the starfish Asterias rubens , Gorshkov et al ( 18 ) detected just two types of cells and termed them “mature” and “immature” coelomocytes.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

et al. 2021

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Coelomocytes is the generic name for a collection of cellular morphotypes, present in many coelomate animals, and highly variable among echinoderm classes. The roles attributed to the major types of these free circulating cells present in the coelomic fluid of echinoderms include immune response, phagocytic digestion and clotting. Our main aim in this study was to characterize coelomocytes found in the coelomic fluid of Marthasterias glacialis (class Asteroidea) by using a combination of flow cytometry (FC), imaging flow cytometry (IFC) and fluorescence plus transmission electron microscopy (TEM). Two coelomocyte populations (P1 and P2) identified through flow cytometry were subsequently studied in terms of abundance, morphology, ultrastructure, cell viability and cell cycle profiles. Ultrastructurally, P2 diploid cells were present as two main morphotypes, similar to phagocytes and vertebrate thrombocytes, whereas the smaller P1 cellular population was characterized by low mitotic activity, a relatively undifferentiated cytotype and a high nucleus/cytoplasm ratio. In the present study we could not rule out possible similarities between haploid P1 cells and stem-cell types in other animals. Additionally, we report the presence of two other morphotypes in P2 that could only be detected by fluorescence microscopy, as well as a morphotype revealed via combined microscopy/FC. This integrative experimental workflow combined cells physical separation with different microscopic image capture technologies, enabling us to better tackle the characterization of the heterogeneous composition of coelomocytes populations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

et al. 2021

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“…To the best of our knowledge, only three studies have used cytocentrifugation to analyze echinoderm coelomocyte morphology (Grand et al, 2014;Queiroz & Custódio, 2015;Taguchi et al, 2016), and all of them showed a high potential to improve cell analyses in Echinodermata. Only phagocytes and small spherical cells were previously known to the sea star Acanthaster planci (Boolotian, 1962).…”

Section: Discussionmentioning

confidence: 99%

“…Although cytocentrifugation dates from the mid-1980s and is a well-established method for cell analyses in clinical medicine, its use is not so widespread among invertebrate scholars, and even less so among echinoderm researchers (Raftos, Gross, & Smith, 2004;Majeske, Oleksyk, & Smith, 2013b). To the best of our knowledge, only three (recent) studies have used cytospin preparations to analyze coelomocyte morphology in Echinodermata (Grand, Pratchett, & Rivera-Posada, 2014;Queiroz & Custódio, 2015;Taguchi, Tsutsui, & Nakamura, 2016).…”

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confidence: 99%

Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy

Queiroz

Arizza

Vazzana

et al. 2021

RBT

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Introduction: Echinoderm coelomocytes have traditionally been investigated through a morphological approach using light microscopy, which relies on the idea of constant cell shape as a stable character. However, this can be affected by biotic or abiotic conditions. Objective: To analyze if the consistency in cell morphology offered by the cytocentrifugation method, might be used as a convenient tool to study echinoderm coelomocytes. Methods: Cells of Echinaster (Othilia) brasiliensis (Asteroidea), Holothuria (Holothuria) tubulosa (Holothuroidea), Eucidaris tribuloides, Arbacia lixula, Lytechinus variegatus, and Echinometra lucunter (Echinoidea) were spread on microscope slides by cytocentrifugation, stained, and analyzed through light microscopy. Additionally, fluorescence microscopy, scanning electron microscopy, and energy-dispersive x-ray spectroscopy were applied to cytospin preparations, to complement the analysis of granular and colorless spherulocytes of Eucidaris tribuloides. Results: Altogether, 11 cell types, including phagocytes, spherulocytes, vibratile cells, and progenitor cells were identified in the samples analyzed. The granular spherulocyte, a newly-described cell type, was observed in all Echinoidea and was very similar to the acidophilic spherulocytes of Holothuria (Holothuria) tubulosa. Conclusions: Cytocentrifugation proved to be versatile, either as the main method of investigation in stained preparations, or as a framework on which other procedures may be performed. Its ability to maintain a constant morphology allowed accurate correspondence between live and fixed/stained cells, differentiation among similar spherulocytes as well as comparisons between similar cells of Holothuroidea and Echinoidea.

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“…A general consensus on echinoderm coelomocyte types is lacking, possibly due to the actual diversity of morphotypes among the different classes ( 93) but mostly to the diversity of techniques and protocols used to analyze them (98). For this reason, from two to up to twelve cytotypes are reported in the literature (97,(99)(100)(101)(102)(103)(104)(105). The most common types are phagocytes, red and white amoebocytes, and vibratile cells (93,97).…”

Section: Echinodermsmentioning

confidence: 99%

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

et al. 2021

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The scopes related to the interplay between stem cells and the immune system are broad and range from the basic understanding of organism’s physiology and ecology to translational studies, further contributing to (eco)toxicology, biotechnology, and medicine as well as regulatory and ethical aspects. Stem cells originate immune cells through hematopoiesis, and the interplay between the two cell types is required in processes like regeneration. In addition, stem and immune cell anomalies directly affect the organism’s functions, its ability to cope with environmental changes and, indirectly, its role in ecosystem services. However, stem cells and immune cells continue to be considered parts of two branches of biological research with few interconnections between them. This review aims to bridge these two seemingly disparate disciplines towards much more integrative and transformative approaches with examples deriving mainly from aquatic invertebrates. We discuss the current understanding of cross-disciplinary collaborative and emerging issues, raising novel hypotheses and comments. We also discuss the problems and perspectives of the two disciplines and how to integrate their conceptual frameworks to address basic equations in biology in a new, innovative way.

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Differential count and time-course analysis of the cellular composition of coelomocyte aggregate of the Japanese sea cucumber Apostichopus japonicus

Cited by 21 publications

References 23 publications

Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach

Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy

Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology

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