Abstract:Summary: Low-temperature scanning electron microscopy (LTSEM) is limited in resolution and image quality by charging of frozen hydrated samples and collection deficiencies of secondary electron signal contrasts. We measured and corrected both effects using differential hysteresis processing (DHP) of LTSEM images, scanned at 15-bit from 5 × 4 inch Polaroid negatives. Bulk charging produced a major contrast component equal to 44-87% of the intensity range of the image. The strong charging contrast reduced the lo… Show more
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