Differential configurations involving binding of USF transcription factors and Twist1 regulate<i>Alx3</i>promoter activity in mesenchymal and pancreatic cells

García‐Sanz, Patricia; Fernández-Pérez, Antonio; Vallejo, Mario

doi:10.1042/bj20120962

Cited by 13 publications

(26 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temporal changes in USF2 and TWIST2 transcript abundance were established and compared to results obtained for USF1 to determine potential association of these transcription factors (Garcia-Sanz et al 2013) in transcriptional regulation of JY-1 , GDF9 and other genes containing multiple E-box binding elements in their promoter regions. In comparison with USF1 , USF2 transcript abundance displayed similar changes during oocyte and early embryonic development (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Most commonly, this transcription factor binds to a pyrimidine-rich initiator (Inr) elements or E-box motifs of the promoter region as homodimers or a heterodimer (USF1/2) and might act with or without association with other E-box binding transcription factors of bHLH family (Garcia-Sanz et al 2013). The USF1/USF2 heterodimer is a major DNA binding form present in most tissues and cell types compared to the homodimers (Fair et al 1995, Rajput et al 2013).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development

et al. 2015

View full text Add to dashboard Cite

Upstream stimulating factor 1 (USF1) is a basic helix loop helix transcription factor that specifically binds to E-box DNA motifs, known cis-elements of key oocyte expressed genes essential for oocyte and early embryo development. However, the functional and regulatory role of USF1 in bovine oocyte and embryo development is not understood. In this study, we demonstrated that USF1 mRNA is maternal in origin and expressed in a stage specific manner during the course of oocyte maturation and preimplantation embryonic development. Immunocytochemical analysis showed detectable USF1 protein during oocyte maturation and early embryonic development with increased abundance at 8–16 cell stage of embryo development, suggesting a potential role in embryonic genome activation. Knockdown of USF1 in germinal vesicle stage oocytes did not impact meiotic maturation or cumulus expansion, but caused significant changes in mRNA abundance for genes associated with oocyte developmental competence. Furthermore, siRNA mediated depletion of USF1 in presumptive zygote stage embryos demonstrated that USF1 is required for early embryonic development to the blastocyst stage. A similar (USF2) yet unique (TWIST2) expression pattern during oocyte and early embryonic development for related E-box binding transcription factors known to cooperatively bind USF1 suggest a potential link to USF1 action. This study demonstrates that USF1 is a maternally derived transcription factor required for bovine early embryonic development which also functions in regulation of JY-1, GDF9 and FST genes associated with oocyte competence.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development

et al. 2015

View full text Add to dashboard Cite

show abstract

“…4b). The addition of ALX3 antiserum 27 disrupted the formation of these complexes, demonstrating the presence of ALX3 bound to the oligonucleotide probes for these three sites (Fig. 4b).…”

Section: Alx3 Represses Mitf By Binding To Its Promotermentioning

confidence: 89%

Developmental mechanisms of stripe patterns in rodents

Mallarino¹,

Hénégar²,

Mirasierra³

et al. 2016

Nature

Self Cite

113

View full text Add to dashboard Cite

Mammalian color patterns are among the most recognizable characters found in nature and can have a profound impact on fitness. However, little is known about the mechanisms underlying their formation and subsequent evolution. Here we show that, in the African striped mouse (Rhabdomys pumilio), periodic dorsal stripes result from underlying differences in melanocyte maturation, which give rise to spatial variation in hair color, and we identify the transcription factor Alx3 as a regulator of this process. In embryonic dorsal skin, patterned expression of Alx3 foreshadows pigment stripes, and acts to directly repress Mitf, a master regulator of melanocyte differentiation, giving rise to light-colored hair. Moreover, Alx3 is also upregulated in the light stripes of chipmunks, which have independently evolved a similar pattern of dorsal stripes. Our results reveal a previously unappreciated mechanism for modulating spatial variation in hair color, and provide new insight into the ways in which phenotypic novelty evolves.

show abstract

“…Other mechanisms have implications with regard to cell growth control. USF1/2 interact with components of the basal transcription complex [Garcia-Sanz et al, 2013] and guide recruitment of the hSET1A histone methylase complex to chromatin as part of lineage-specifc cell differentiation [Deng et al, 2013]. Recent findings suggest, moreover, that USF2 is required for induction of HIF2α responsive genes (including PAI-1) under hypoxic conditions likely involving recruitment of the histone acetylases p300 and CBP [Palmeri et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

The Basic Helix‐Loop‐Helix/Leucine Zipper Transcription Factor USF2 Integrates Serum‐Induced PAI‐1 Expression and Keratinocyte Growth

Higgins

et al. 2014

J of Cellular Biochemistry

View full text Add to dashboard Cite

Plasminogen activator inhibitor type-1 (PAI-1), a major regulator of the plasmin-dependent pericellular proteolytic cascade, is prominently expressed during the tissue response to injury although the factors that impact PAI-1 induction and their role in the repair process are unclear. Kinetic modeling using established biomarkers of cell cycle transit (c-MYC; cyclin D1; cyclin A) in synchronized human (HaCaT) keratinocytes, and previous cytometric assessments, indicated that PAI-1 transcription occurred early after serum-stimulation of quiescent (G0) cells and prior to G1 entry. It was established previously that differential residence of USF family members (USF1→USF2 switch) at the PE2 region E box (CACGTG) characterized the G0→G1 transition period and the transcriptional status of the PAI-1 gene. A consensus PE2 E box motif (5′-CACGTG-3′) at nucleotides -566 to -561 was required for USF/E box interactions and serum-dependent PAI-1 transcription. Site-directed CG→AT substitution at the two central nucleotides inhibited formation of USF/probe complexes and PAI-1 promoter-driven reporter expression. A dominant-negative USF (A-USF) construct or double-stranded PE2 “decoy” attenuated serum- and TGF-β1-stimulated PAI-1 synthesis. Tet-Off induction of an A-USF insert reduced both PAI-1 and PAI-2 transcripts while increasing the fraction of Ki-67+ cells. Conversely, overexpression of USF2 or adenoviral-delivery of a PAI-1 vector inhibited HaCaT colony expansion indicating that the USF1→USF2 transition and subsequent PAI-1 transcription are critical events in the epithelial go-or-grow response. Collectively, these data suggest that USF2, and its target gene PAI-1, regulate serum-stimulated keratinocyte growth, and likely the cadence of cell cycle progression in replicatively-competent cells as part of the injury repair program.

show abstract

Differential configurations involving binding of USF transcription factors and Twist1 regulateAlx3promoter activity in mesenchymal and pancreatic cells

Cited by 13 publications

References 56 publications

Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development

Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development

Developmental mechanisms of stripe patterns in rodents

The Basic Helix‐Loop‐Helix/Leucine Zipper Transcription Factor USF2 Integrates Serum‐Induced PAI‐1 Expression and Keratinocyte Growth

Contact Info

Product

Resources

About