Differential cellular reactivity to adult worm antigens of patients with different clinical forms of schistosomiasis mansoni

Bahia-Oliveira, Lilian M. G.; Gazzinelli, Giovanni; Elói-Santos, Silvana Maria; Cunha-Melo, José Renan; Alves-Oliveira, L. F.; Silveira, Alda Maria Soares; Viana, Iramaya R. C.; Carmo, Julianderson de Oliveira dos Santos; Souza, Anderson Reis de; Correa-Oliveira, Rodrigo

doi:10.1016/0035-9203(92)90441-e

Cited by 30 publications

(23 citation statements)

References 23 publications

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“…Analysis of IFN-γ secretion in supernatants of tissue cultures demonstrated that, as described for the INT group, very low to undetectable levels of this cytokine are secreted in PBMC cultures stimulated with the parasite antigens (24).…”

Section: Hepatosplenic Form Of Schistosomiasismentioning

confidence: 61%

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection

Corrêa-Oliveira

Malaquias

Falcão

et al. 1998

Braz J Med Biol Res

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The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals naturally resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT or HS. In contrast, the addition of anti-IL-10 antibodies to PBMC cultures from INT patients significantly increased the proliferative response to SEA and SWAP as well as the in vitro granuloma formation. Interestingly, association of anti-IL-4 and anti-IL-10 antibodies did not increase the PBMC proliferative response of these patients, suggesting that IL-10 may act by modulating IL-4 and IL-5 secretion. Addition of recombinant IL-10 decreased the proliferative response to undetectable levels when PBMC from patients with the different clinical forms were used. Analysis of IFN-γ in the supernatants showed that PBMC from INT patients secreted low levels of IFN-γ upon antigenic stimulation. In contrast, PBMC from NE secreted high levels of IFN-γ. These data suggest that IL-10 is an important cytokine in regulating the immune response and possibly controlling morbidity in human schistosomiasis mansoni, and that the production of IFN-γ may be associated with resistance to infection.

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Section: Hepatosplenic Form Of Schistosomiasismentioning

confidence: 61%

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection

Corrêa-Oliveira

Malaquias

Falcão

et al. 1998

Braz J Med Biol Res

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“…29 Moreover, when stimulated with Schistosoma antigens, peripheral blood mononuclear cells (PBMC) of EN persons proliferate vigorously and produce higher levels of IFN-g than those of persons currently infected and those resistant to reinfection. 28,30,31 On the basis of this evidence, it appears that Th1 and Th2 immune response are involved in protection against the infection in this naturally resistant group of persons.…”

Section: Introductionmentioning

confidence: 92%

Factors Associated with Resistance to Schistosoma mansoni Infection in an Endemic Area of Bahia, Brazil

Oliveira¹,

Figueiredo²,

Cardoso³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Detailed knowledge of factors associated with resistance to Schistosoma mansoni infection in endemic areas might facilitate more effective schistosomiasis control. We conducted a cross-sectional study of persons resistant to schistosomiasis and found no association between socioeconomic status and resistance to infection. Mononuclear cells of resistant subjects produced higher levels of interleukin-5 (IL-5), IL-13 and interferon-g upon stimulation with soluble egg antigen (SEA) compared with infected persons. When stimulated with Sm21.6 or Sm22.6, levels of IL-10 were higher in cell culture of resistant persons. Levels of IgE against soluble adult worm antigen (SWAP) and against interleukin-4-inducing principle from S. mansoni eggs (IPSE) and levels of IgG4 against SWAP, SEA, and Sm22.6 were lower in the resistant group compared with the susceptible group. Our data suggest that socioeconomic status could not fully explain resistance to S. mansoni infection observed in the studied area. However, a mixture of Th1 and Th2 immune responses and low levels of specific IgG4 against parasite antigens could be mediating resistance to infection.

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“…It is not yet clear whether this observed pattern is the result of changes in behavior or acquired immunity as the endemic population ages. 1,2 Recently, both Webster and coworkers 3 and our group 4,5 have reported that another rise in infection intensity occurs among the elderly, suggesting a loss of this partially acquired immunity by aged people. To date, there has been no systematic study of the immune response of individuals residing in S. mansoni endemic areas over the age of 60 years.…”

Section: Introductionmentioning

confidence: 99%

Production of interferon-γ by natural killer cells and aging in chronic human schistosomiasis

Speziali

Bethony

Martins‐Filho

et al. 2004

Mediators of Inflammation

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BACKGROUND : Aging is associated with several alterations in the phenotype, repertoire and activation status of lymphocytes as well as in the cytokine profile produced by these cells. As a lifelong condition, chronic parasitic diseases such as human schistosomiasis overlaps with the aging process and no systematic study has yet addressed the changes in immune response during infection with Schistosoma mansoni in older individuals. Aim : Herein we study the influence of immunological alterations brought about by senescence in the course of schistosomiasis.Materials and methods : Individuals 10 Á /95 years of age, from both sexes, from an endemic area for S. mansoni infection were matched by intensity of infection as measured by egg counts. We analyzed, as a parameter, cytokine expression by lymphocytes and natural killer cells after in vitro stimulation with soluble egg antigen and soluble worm antigen using flow cytometry. Results : We demonstrated that the frequency of CD16 interferon-g (IFN-g) natural killer cells in negative individuals over the age of 70 years is significantly higher than in positive individuals after in vitro stimulation with S. mansoni antigen extracts. The frequency of these cells is increased in all individuals over the age of 50 years and only declines in positive individuals after 70 years of age. Analysis of either CD4? or CD8 ? cells after antigen stimulation show no significant increase in frequency of IFN-g in negative or in positive individuals of this age group, suggesting that the effect on CD16 cells is not T-cell dependent. Conclusion : Since production of IFN-g has been related to resistance to schistosome infection, our data suggest that age-associated changes in CD16 cells may play a role in controlling infection intensity in the elderly in S. mansoni endemic areas of Brazil.

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Differential cellular reactivity to adult worm antigens of patients with different clinical forms of schistosomiasis mansoni

Cited by 30 publications

References 23 publications

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection

Factors Associated with Resistance to Schistosoma mansoni Infection in an Endemic Area of Bahia, Brazil

Production of interferon-γ by natural killer cells and aging in chronic human schistosomiasis

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