Differential cellular interactions and responses to ultrathin micropatterned graphene oxide arrays with or without ordered in turn RGD peptide films

Shin, Min Chan; Kang, Moon Sung; Park, Rowoon; Chae, Seon Yeong; Han, Dong‐Wook; Hong, Suck Won

doi:10.1016/j.apsusc.2021.150115

Cited by 12 publications

(20 citation statements)

References 48 publications

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“…The stacked GO sheets on the glass substrate were functionalized with two different biomolecules, such as RGD peptide and AST. RGD peptide was covalently bonded on GO thin film as previously reported for form RGD-GO complex [ 27 ]. First, 200 μL aqueous solution consisting of 260 μM of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and 520 μM of N-hydroxysuccinimide (NHS), were dropped onto the GO thin film for 1 h. Following that, the activated GO thin film was carefully rinsed with DI water.…”

Section: Methodsmentioning

confidence: 99%

“…Particularly, the edge of GO sheets can physically damage bacterial membrane, and inactivate bacteria because of leakage of the intracellular matrix [ 26 ]. RGD peptide is a major cell adhesion peptide as a bioactive ligand that improves cellular behavior by regulating cell adhesion, migration, proliferation, and differentiation [ 27 ]. Furthermore, it is known as a primary recognition motif of ECM protein containing three sequential amino acids, and the functionalization of the RGD peptide can improve cell adhesion and growth through surface-mediated interactions between cells and substrates [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

2022

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Background Astaxanthin (AST) is known as a powerful antioxidant that affects the removal of active oxygen and inhibits the production of lipid peroxide caused by ultraviolet light. However, it is easily decomposed by heat or light during production and storage because of the unsaturated compound nature with a structural double bond. The activity of AST can be reduced and lose its antioxidant capability. Graphene oxide (GO) is an ultrathin nanomaterial produced by oxidizing layered graphite. The chemical combination of AST with GO can improve the dispersion properties to maintain structural stability and antioxidant activity because of the tightly bonded functionalized GO surface. Methods Layered GO films were used as nanocarriers for the AST molecule, which was produced via flow-enabled self-assembly and subsequent controlled solution deposition of RGD peptide and AST molecules. Synthesis of the GO-AST complex was also carried out for the optimized concentration. The characterization of prepared materials was analyzed through transmission electron microscopy (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscope (AFM), and Raman spectroscopy. Antioxidant activity was tested by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2.2-diphenyl-1-picrylhydrazyl (DPPH) assays. The antibacterial effect and antioxidant effects were monitored for the ultrathin GO/RGD/AST Film. Further, reactive oxygen species (ROS) assay was used to evaluate the anti-inflammatory effects on L-929 fibroblasts. Results Cotreatment of GO-AST solution demonstrated a high antioxidant combined effect with a high ABTS and DPPH radicals scavenging activity. The GO/RGD/AST film was produced by the self-assembly process exhibited excellent antibacterial effects based on physicochemical damage against E. coli and S. aureus. In addition, the GO/RGD/AST film inhibited H2O2-induced intracellular ROS, suppressed the toxicity of lipopolysaccharide (LPS)-induced cells, and restored it, thereby exhibiting strong antioxidant and anti-inflammatory effects. Conclusion As GO nanocarrier-assisted AST exerted promising antioxidant and antibacterial reactions, presented a new concept to expand basic research into the field of tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

2022

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“…The Prewitt method is the development of the Robert method using an HPF filter which is given a buffer zero [18]. Robert's method is another name for the differential technique in the horizontal direction and the differential in the vertical direction, with the addition of a binary conversion process after the differential is performed [19].…”

Section: Size=imresize (Graysacle[256 256]);mentioning

confidence: 99%

Image Edge Detection Capture Zoom for Facial Recognition Using Gradient Operators

Hadi

Sumijan

2022

komtekinfo

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Imagery on edge detection is a process that will display the edges of an image. Basically, edge detection is one of the techniques for the analysis of image quality in the spatial domain and is also one of the initial process in digital image processing. Edge detection serves to detect the border line of an object contained in the image. This study aims to identify and recognize face pattern objects in the capture zoom image. To perform face identification begins with collecting image data, image processing, image edge detection, thinning of the image, and identification process using the template matching method. The method used in edge detection uses 3 methods, namely Sobel, Roberts and Prewitt which are gradient operators to detect edges in facial images. The dataset used is image capture zoom. The trial was carried out in two stages, namely the identification of the face shape and the identification of the edges of the face. The conclusion of the study is that the Roberts operator is the operator that finds the least edge patterns in facial images than the other two operators, namely Prewitt and Sobel. Meanwhile, the Sobel operator produces edge patterns that are better in quality and quantity than using the Roberts and Prewitt operators.

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“…To our best knowledge, an experimental approach for the direct incorporation of PLCL/Col matrices with MXene NPs is extremely rare although PLCL has been widely utilized as a scaffold in advanced tissue engineering mainly due to its exceptional tissue compatibility, rubber-like elasticity, and suitable degradability [ 29 – 31 ]. The main drawback in the use of the PLCL has lied in lack of affinity to the cells, but some other intermediating biomolecules, such as collagen (Col), fibronectin, or RGD peptides, have been utilized to efficiently help a cell adhesion to the prepared matrices [ 32 , 33 ]. Therefore, by adding the monomeric form of type I Col in the matrix [ 34 ], we developed a MXene-decorated PLCL/Col nanostructured material as a novel scaffold toward BTE.…”

Section: Introductionmentioning

confidence: 99%

Ternary MXene-loaded PLCL/collagen nanofibrous scaffolds that promote spontaneous osteogenic differentiation

Lee

Jeon

et al. 2022

Nano Convergence

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Conventional bioinert bone grafts often have led to failure in osseointegration due to low bioactivity, thus much effort has been made up to date to find alternatives. Recently, MXene nanoparticles (NPs) have shown prominent results as a rising material by possessing an osteogenic potential to facilitate the bioactivity of bone grafts or scaffolds, which can be attributed to the unique repeating atomic structure of two carbon layers existing between three titanium layers. In this study, we produced MXene NPs-integrated the ternary nanofibrous matrices of poly(L-lactide-co-ε-caprolactone, PLCL) and collagen (Col) decorated with MXene NPs (i.e., PLCL/Col/MXene), as novel scaffolds for bone tissue engineering, via electrospinning to explore the potential benefits for the spontaneous osteogenic differentiation of MC3T3-E1 preosteoblasts. The cultured cells on the physicochemical properties of the nanofibrous PLCL/Col/MXene-based materials revealed favorable interactions with the supportive matrices, highly suitable for the growth and survival of preosteoblasts. Furthermore, the combinatorial ternary material system of the PLCL/Col/MXene nanofibers obviously promoted spontaneous osteodifferentiation with positive cellular responses by providing effective microenvironments for osteogenesis. Therefore, our results suggest that the unprecedented biofunctional advantages of the MXene-integrated PLCL/Col nanofibrous matrices can be expanded to a wide range of strategies for the development of effective scaffolds in bone tissue regeneration. Graphical Abstract

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Differential cellular interactions and responses to ultrathin micropatterned graphene oxide arrays with or without ordered in turn RGD peptide films

Cited by 12 publications

References 48 publications

Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

Image Edge Detection Capture Zoom for Facial Recognition Using Gradient Operators

Ternary MXene-loaded PLCL/collagen nanofibrous scaffolds that promote spontaneous osteogenic differentiation

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