Differential Cell Surface Expression of the STRO-1 and Alkaline Phosphatase Antigens on Discrete Developmental Stages in Primary Cultures of Human Bone Cells

Gronthos, Stan; Zannettino, Andrew C.W.; Graves, Stephen; Ohta, Shuichi; Hay, Shelley; Simmons, Paul J.

doi:10.1359/jbmr.1999.14.1.47

Cited by 262 publications

(243 citation statements)

References 54 publications

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“…In culture, cells expressing Stro-1 À /ALP + accounted for approximately 40% of the population and Stro-1 + /ALP + a further 10%. These values are consistent with early passage cultured cells derived from known MSC sources: trabecular bone explants [17], bone marrow, synovial lining, adipose tissue, muscle, and a single passage of periosteum [35]. More surprising than the osteogenic phenotype of cultured HPDCs in this study was the presence of a consistent and stable Stro-1 + population.…”

Section: Discussionsupporting

confidence: 85%

“…Initial cell isolates consistently contained large populations of CD34 + hematopoietic cells and ALP + osteogenic cell types, small numbers of smooth muscle, endothelial and adipocytic cells, and small subpopulations of Stro-1 + cell types. Based on the study of developmental phenotypes of stromal cell cultures, it has been suggested that Stro-1 + /ALP À cells represent a less differentiated osteoblastic phenotype, Stro-1 + /ALP + cells an intermediate stage, and Stro-1 À /ALP + cells the most mature [17]. These findings build on work by Lim [24], who identified potential precursor populations within periosteal cultures.…”

Section: Discussionmentioning

confidence: 72%

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Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering. Questions/purpose We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro. Methods We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1 À and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription-polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization. Results Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 72%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…After 4 weeks of induction, cultures were either stained with Alizarin red solution or analyzed for levels of extracellular calcium deposits (5 Â 10 3 MSC per well in 96-well plates, quadruplicate cultures) in the presence of o-cresol-phthalein-complexon (Sigma) with an absorbance read at 570 nm as previously described [5,26]. Adipogenesis was induced as previously described [5].…”

Section: In Vitro Differentiation Assaysmentioning

confidence: 99%

TWIST Family of Basic Helix-Loop-Helix Transcription Factors Mediate Human Mesenchymal Stem Cell Growth and Commitment

et al. 2009

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The TWIST family of basic helix-loop-helix transcription factors, Twist-1 and Dermo-1 are known mediators of mesodermal tissue development and contribute to correct patterning of the skeleton. In this study, we demonstrate that freshly purified human bone marrow-derived mesenchymal stromal/stem cells (MSC) express high levels of Twist-1 and Dermo-1 which are downregulated following ex vivo expansion. Enforced expression of Twist-1 or Dermo-1 in human MSC cultures increased expression of the MSC marker, STRO-1, and the early osteogenic transcription factors, Runx2 and Msx2. Conversely, overexpression of Twist-1 and Dermo-1 was associated with a decrease in the gene expression of osteoblast-associated markers, bone morphogenic protein-2, bone sialoprotein, osteopontin, alkaline phosphatase and osteocalcin. High expressing Twist-1 or Dermo-1 MSC lines exhibited an enhanced proliferative potential of approximately 2.5-fold compared with control MSC populations that were associated with elevated levels of Id-1 and Id-2 gene expression. Functional studies demonstrated that high expressing Twist-1 and Dermo-1 MSC displayed a decreased capacity for osteo/chondrogenic differentiation and an enhanced capacity to undergo adipogenesis. These findings implicate the TWIST gene family members as potential mediators of MSC self-renewal and lineage commitment in postnatal skeletal tissues by exerting their effects on genes involved in the early stages of bone development.

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“…Human mesenchymal stem cells (MSC), which are first isolated from bone marrow (BM) [1,2] and subsequently isolated from other tissues such as adipose tissue, cutaneous tissue, fetal hepatic and pulmonary tissue [3][4][5][6][7][8][9][10][11], are pluripotent progenitors for a variety of tissues including bone, cartilage, tendon, fat, and muscle [12]. It has been approved have the capability to support expansion of hematopoietic stem cells (HSC) through expressing cytokines and reconstructing hematopoietic microenvironment [13,14].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation

Zhang

et al. 2005

Cell Res

111

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Differential Cell Surface Expression of the STRO-1 and Alkaline Phosphatase Antigens on Discrete Developmental Stages in Primary Cultures of Human Bone Cells

Abstract: ABSTRACT

Cited by 262 publications

References 54 publications

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

TWIST Family of Basic Helix-Loop-Helix Transcription Factors Mediate Human Mesenchymal Stem Cell Growth and Commitment

Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation

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