Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus

Dai, Shiyu; Wu, Qiaoli; Wu, Xiaoli; Peng, Cheng Yuan; Liu, Jia; Táng, Shuāng; Zhang, Tao; Deng, Fei; Shěn, Shū

doi:10.3389/fcimb.2021.648077

Cited by 21 publications

(23 citation statements)

References 24 publications

(52 reference statements)

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“…Here, we have applied global blood transcriptomics in longitudinal samples collected during the acute phase of CCHFV-infection and the convalescent phase (nearly after a year of recovery) to measure the system-wide changes during the CCHFV-infection in patients from Turkey. We also performed temporal quantitative proteomics analysis to understand the cellular alterations during the productive CCHFV-infection in two different cell lines, human adrenal carcinoma cell line, SW13, and human liver cell line Huh7 that were reported to be the most permissive cell lines for CCHFV ( Dai et al, 2021 ). Using the newly gained insights, we then modulated the critical pathways by drugs to halt the productive CCHFV-replication in in vitro infection models.…”

Section: Introductionmentioning

confidence: 99%

Multi-omics insights into host-viral response and pathogenesis in Crimean-Congo hemorrhagic fever viruses for novel therapeutic target

Neogi

Elaldı

Appelberg

et al. 2022

eLife

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The pathogenesis and host-viral interactions of the Crimean–Congo hemorrhagic fever orthonairovirus (CCHFV) are convoluted and not well evaluated. Application of the multi-omics system biology approaches, including biological network analysis in elucidating the complex host-viral response, interrogates the viral pathogenesis. The present study aimed to fingerprint the system-level alterations during acute CCHFV-infection and the cellular immune responses during productive CCHFV-replication in vitro. We used system-wide network-based system biology analysis of peripheral blood mononuclear cells (PBMCs) from a longitudinal cohort of CCHF patients during the acute phase of infection and after one year of recovery (convalescent phase) followed by untargeted quantitative proteomics analysis of the most permissive CCHFV-infected Huh7 and SW13 cells. In the RNAseq analysis of the PBMCs, comparing the acute and convalescent-phase, we observed system-level host’s metabolic reprogramming towards central carbon and energy metabolism (CCEM) with distinct upregulation of oxidative phosphorylation (OXPHOS) during CCHFV-infection. Upon application of network-based system biology methods, negative coordination of the biological signaling systems like FOXO/Notch axis and Akt/mTOR/HIF-1 signaling with metabolic pathways during CCHFV-infection were observed. The temporal quantitative proteomics in Huh7 showed a dynamic change in the CCEM over time and concordant with the cross-sectional proteomics in SW13 cells. By blocking the two key CCEM pathways, glycolysis and glutaminolysis, viral replication was inhibited in vitro. Activation of key interferon stimulating genes during infection suggested the role of type I and II interferon-mediated antiviral mechanisms both at the system level and during progressive replication.

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Section: Introductionmentioning

confidence: 99%

Multi-omics insights into host-viral response and pathogenesis in Crimean-Congo hemorrhagic fever viruses for novel therapeutic target

Neogi

Elaldı

Appelberg

et al. 2022

eLife

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“…E-11 and GF-1 cells provided the highest production rates for RGNNV and RG/SJ (around 10 3 ). If permissive cell lines are defined as those producing 100-fold viral increases above the inoculum [32], both cell lines can be regarded as fully permissive for these viral types. The SJNNV production rate was lower in both cells, which could be due to the incubation temperature used, as it has been reported that at 25 • C this strain shows reduced fitness [33].…”

Section: Discussionmentioning

confidence: 99%

Differential Nervous Necrosis Virus (NNV) Replication in Five Putative Susceptible Cell Lines

et al. 2021

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Viral encephalopathy and retinopathy caused by nervous necrosis virus (NNV), is one of the most threatening viral diseases affecting marine fish worldwide. In vitro propagation of NNV strains is essential for the design of effective control measures. In the present study we analysed both the susceptibility and the permissiveness of five fish cell lines (E-11, GF-1, SAF-1, DLB-1, and SaB-1) to three NNV strains (one RGNNV, one SJNNV, and one reassortant RGNNV/SJNNV). E-11 and DLB-1 were demonstrated to be highly susceptible to NNV strains, with average adsorption efficiency (AE) values higher than 90%. SAF-1 also showed high susceptibility (AE 88%), whereas GF-1 can be regarded as moderately susceptible (AE around 50%). On the contrary, SaB-1 can be considered a poorly susceptible cell line (AE values below 20%). E-11 and GF-1 cell lines provided the highest production rates for RGNNV and RG/SJ (around 103) and both cell lines can be regarded as fully permissive for these viral types. However, the SJNNV production rate in GF-1 was only 17.8 and therefore this cell line should be considered semi-permissive for this genotype. In SAF-1 cells, moderate viral replication was recorded but differences in intracellular and extracellular production suggest that viral progeny was not efficiently released. In DLB-1 and SaB-1 the final viral titres obtained in E-11 were lower than those of the inoculum. However, RNA1 synthesis values seem to indicate that RGNNV replication in DLB-1 and SAF-1 could have been underestimated, probably due to a poor adaptation of the virus grown in these cell lines to E-11. Based on all these results, E-11 seems to be the most appropriate cell for in vitro culture of RGNNV, SJNNV, and reassortant strains.

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“…CCHFV replicates in various cell lines, such as Vero, CER, SW13 and BHK21, but does not usually yield high titers [ 17 ]. The virus is poorly cytopathic in cell culture, so titers may be demonstrated by indirect immunofluorescence in infected cells [ 18 ].…”

Section: Cchfv Genomementioning

confidence: 99%

Crimean-Congo Hemorrhagic Fever Virus in Asia, Africa and Europe

et al. 2021

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The global spread of ticks and various tick-borne viruses (TBVs) suggests the possibility of new tick-borne diseases emerging. Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging TBV of the Nairoviridae family that causes serious disease that can be fatal in humans. CCHFV endemic foci can be found in Africa, Asia, the Middle East, and South-Eastern Europe, and has spread to previously unaffected regions and nations, such as Spain, over the last two decades. In this review, we discuss the current situation of CCHFV in Asia, Africa and Europe based on existing knowledge, and we discuss driving factors in the distribution and transmission of the virus, such as the spread of tick vector species and host reservoirs.

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Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus

Cited by 21 publications

References 24 publications

Multi-omics insights into host-viral response and pathogenesis in Crimean-Congo hemorrhagic fever viruses for novel therapeutic target

Multi-omics insights into host-viral response and pathogenesis in Crimean-Congo hemorrhagic fever viruses for novel therapeutic target

Differential Nervous Necrosis Virus (NNV) Replication in Five Putative Susceptible Cell Lines

Crimean-Congo Hemorrhagic Fever Virus in Asia, Africa and Europe

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