“…34,35 To investigate TRAIL-mediated effects, leukemic cells were coincubated with 1 g/ml of soluble, recombinant, human TRAIL (N-terminally tagged with the FLAG epitope; Alexis, San Diego, CA, USA) and trimerized with a FLAG-specific antibody (2 g/ml; Alexis) as described elsewhere. 13 To assess a possible sensitization to TRAIL-induced apoptosis of leukemic cells by the chemotherapeutic agent doxorubicin (Pharmacia & Upjohn, Erlangen, Germany), cells were coincubated with doxorubicin at different concentrations (0.5, 2.5 and 12.5 g/ml) as well as with both TRAIL and doxorubicin, following the above described procedure.…”