Differential binding of two monoclonal antibodies directed against the chicken CD8α molecule

Breed, Dominique G.J.; Carr, Patricia; Vermeulen, A.N.

doi:10.1016/0165-2427(95)05531-2

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…For detection of CD8␣ + cells a mix of mAb EP72 (Southern Biotech) and AV14 (kind gift of Dr T.F. Davison, Institute for Animal Health, Compton UK [39]) was used to avoid differences in staining due to polymorphism in the chicken CD8␣ molecule [3,22].…”

Section: Immunocytochemistrymentioning

confidence: 99%

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Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: A transcriptomics analysis of adjuvanted vaccines

Reemers

Jansen

Koerkamp

et al. 2010

Vaccine

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To gain more insight in underlying mechanisms correlating to protection against avian influenza virus (AIV) infection, we investigated correlates of protection after AIV H9N2 infection and studied the contribution of different adjuvants to a protective response at host transcriptional level. One-day-old chickens were immunised with inactivated H9N2 supplemented with w/o, Al(OH)(3), CpG or without adjuvant. Two weeks later, birds were homologously challenged and at 1-4 days post challenge (d.p.c.) trachea and lung were collected. Birds immunised with H9N2+w/o or H9N2+Al(OH)(3) were protected against challenge infection and had lower viral RNA expression, less immune related genes induced after challenge, a lower amplitude of change of gene expression and smaller cellular influxes compared to the higher and prolonged gene expression in unprotected birds. We show that a limited number of differentially expressed genes correlates with reduced immune activation and subsequently reduced immunopathology after challenge with AIV.

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Section: Immunocytochemistrymentioning

confidence: 99%

“…S2). For w/o 41 unique genes were found and for Al(OH) 3 35 genes. Again expression rates of the these genes were lower in IC + w/o or IC + Al(OH) 3 birds compared to IC birds.…”

Section: Pathway Analysis Of Immune Related Genesmentioning

confidence: 99%

Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: A transcriptomics analysis of adjuvanted vaccines

Reemers

Jansen

Koerkamp

et al. 2010

Vaccine

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“…KUL-01 + cells (macrophages; Mast et al, 1998) and CD4 + cells were detected with monoclonal antibodies KUL-01 and CT-4 (Southern Biotech). For the detection of CD8a + cells, a mixture of mAbs EP72 (Southern Biotech) and AV14 (a kind gift of Dr T. F. Davison, Institute for Animal Health, Compton, UK; Withers et al, 2005) was used to avoid differences in staining due to polymorphism in the chicken CD8a molecule (Breed et al, 1996;Luhtala et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow

Reemers

Haarlem

Koerkamp

et al. 2009

Journal of General Virology

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Sampling the complete organ instead of defined parts might affect analysis at both the cellular and transcriptional levels. We defined host responses to H9N2 avian influenza virus (AIV) in trachea and different parts of the lung. Chickens were spray-inoculated with either saline or H9N2 AIV. Trachea and lung were sampled at 1 and 3 days post-inoculation (p.i.) for immunocytochemistry, real-time quantitative RT-PCR and gene-expression profiling. The trachea was divided into upper and lower parts and the lung into four segments, according to anatomy and airflow. Two segments contained the primary and secondary bronchi, cranial versus caudal (parts L1 and L3), and two segments contained the tertiary bronchi, cranial versus caudal (parts L2 and L4). Between the upper and lower trachea in both control and infected birds, minor differences in gene expression and host responses were found. In the lung of control birds, differences in anatomy were reflected in gene expression, and in the lung of infected birds, virus deposition enhanced the differences in gene expression. Differential gene expression in trachea and lung suggested common responses to a wide range of agents and site-specific responses. In trachea, site-specific responses were related to heat shock and lysozyme activity. In lung L1, which contained most virus, site-specific responses were related to genes involved in innate responses, interleukin activity and endocytosis. Our study indicates that the anatomy of the chicken lung must be taken into account when investigating in vivo responses to respiratory virus infections.

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“…CD8 may occur as an αα homodimer or an αβ heterodimer. CD8αβ is found on cytotoxic T cells while CD8αα is found on a number of small subsets including TCR0 NK cells (Abbas et al, 1991 (Breed et al, 1996).…”

Section: A Population Of Tcr0 Cells ?mentioning

confidence: 99%

Lymphocytic infiltration in the chicken trachea in response to Mycoplasma gallisepticum infection

et al. 2000

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A prominent feature of disease induced by Mycoplasma gallisepticum is a lymphoproliferative response in the respiratory tract. Although this is also seen in other mycoplasma infections, including Mycoplasma pneumoniae, the phenotype of the lymphocytes infiltrating the respiratory tract has not been determined. In this study, the numbers and distribution of lymphocytes in the tracheas of chickens infected with a virulent strain of M. gallisepticum were examined. Three groups of chickens were experimentally infected with M. gallisepticum and three unchallenged groups were used as controls. One infected and one control group were culled at 1, 2 and 3 weeks post infection.

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Differential binding of two monoclonal antibodies directed against the chicken CD8α molecule

Cited by 11 publications

References 19 publications

Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: A transcriptomics analysis of adjuvanted vaccines

Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: A transcriptomics analysis of adjuvanted vaccines

Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow

Lymphocytic infiltration in the chicken trachea in response to Mycoplasma gallisepticum infection

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