Differential binding of alkylating fluorochromes in human chromosomes

Caspersson, T.; Zech, L.; Johansson, C.

doi:10.1016/0014-4827(70)90523-9

Cited by 578 publications

(167 citation statements)

References 8 publications

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“…Another relatively early report suggested the possible existence of the Ph chromosome in the case of a 35-year-old petroleum chemist with chronic myeloid leukemia (CML); however, this case report was not a cytogenetic study (Liaudet and Combaz, 1973). Banding technology, developed in 1970, has allowed the detection of specific structural changes on specific chromosomes (Caspersson et al, 1970). For example, a translocation involving the MLL gene, t(4;11)(q21;q23), was detected in 100% of lymphocytes examined from a benzeneexposed patient who developed early-B ALL.…”

Section: E Specific Translocations and Deletionsmentioning

confidence: 99%

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

Zhang¹,

Eastmond

Smith³

2002

Critical Reviews in Toxicology

151

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Section: E Specific Translocations and Deletionsmentioning

confidence: 99%

The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene

Zhang¹,

Eastmond

Smith³

2002

Critical Reviews in Toxicology

151

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“…Banded preparations were made by both the Giemsa method of Seabright (1971) and the fluorescence method of Caspersson, Zech and Johansson, (1970 (1967) and further purified by the method of Sambrook et al (1968). Finally, the DNA concentration was estimated from the optical density and the DNA stored at -700 in 10-/Ig aliquots.…”

mentioning

confidence: 99%

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease

Webb¹,

Harding²

1977

Br J Cancer

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A comparative study has been made of fibroblasts obtained from patients with differing susceptibilities to malignant disease, both with respect to their chromosome complements and their transformation with SV40 virus. Fibroblasts from 2 Bloom's syndrome patients were found not to have raised SV40 transformation rates and no correlation was found between chromosome abnormality per se and transformation. Of 2 cell types with greatly increased rates, one was derived from a neurofibromatosis patient and the other from an A-T heterozygote. When SV40 DNA was employed as the transforming agent for the latter, the transformation rate was no longer raised. Images Fig. 1 Fig. 2

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“…It first became routine in the 1970s to distinguish each of the 24 different human chromosomes when methods for recognizing substructural bands were reported, notably Q(uinacrine)-banding 7 and trypsin G-banding 8 , which enabled identification of the 'Philadelphia chromosome' (Ph) marker in chronic myeloid leukemia (CML) and its formation through a reciprocal translocation, t(9;22)(q34;q11) 9 . This observation directly led to cancerogenesis being attributed to specific gene alterations.…”

Section: Cytogenetic Methodologiesmentioning

confidence: 99%