Differential assay of A and B isoenzymes in urinary N-acetyl-β-d-glucosaminidase

Paraire, Marie; Bourbouze, Richard; Baumann, Fritz; Percheron, F

doi:10.1016/0009-8981(83)90222-x

Cited by 20 publications

(8 citation statements)

References 15 publications

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“…Activity of NAG-B isoenzyme was measured by a batchwise method [12]. NAG-A isoenzyme was bound to DEAE-52 cellulose; the unbound NAG-B isoenzyme was measured in the supernatant by the fluorimetric method that was described above.…”

Section: Methodsmentioning

confidence: 99%

Etiology of Increased Enzymuria in Different Morphological Forms of Glomerulonephritis

Kuźniar¹,

Marchewka²,

Lembas-Bogaczyk³

et al. 2004

Nephron Physiol

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Background: High urinary excretion of lysosomal enzymes is thought to reflect tubulointerstitial damage and is observed both in the acute and chronic phases of various morphological forms of glomerulonephritis (GN). It is related to the degree of proteinuria and secondary interstitial inflammatory process. N-acetyl-β-D-glucosaminidase (NAG) and β-glucuronidase (β-GR) are the most commonly used markers of tubulointerstitial injury. NAG and β-GR are also contained in azurophilic granulations of polymorphonuclear leukocytes (PMNs) and may be released during the activation of PMNs. Aims: The aim of this study was to elucidate the role of PMN degranulation in causing the increase of urinary excretion of lysosomal enzymes that is observed in glomerulonephritis. Material and Methods: We analyzed the urinary excretion of NAG, its B isoenzyme NAG-B, β-GR and leukocyte elastase (EL), in 91 patients with morphologically different primary and secondary glomerulopathies, and in 12 healthy controls. Results: Excretion of NAG, NAG-B and β-GR were statistically significantly higher in all GN patients in comparison to healthy controls. In the whole analyzed GN population significant correlations between amount of proteinuria and excretion of NAG, NAG-B and β-GR were ascertained. In subgroup analysis NAG excretion was significantly correlated with proteinuria in patients with diffuse proliferative GN (PGN), mesangiocapillary GN (MCGN), and minimal change disease (MCD). There was a significant correlation between NAG-B and proteinuria in MCD and PGN patients. There was a significant relationship of β-GR and EL with proteinuria and EL with NAG in the PGN group. Significant relationships between serum creatinine and excretion of EL but not NAG, NAG-B, or β-GR were observed in the whole examined group. Conclusions: Increased urinary excretion of elastase, with concomitant high proteinuria and NAG excretion in patients with proliferative GN may indicate that leukocyte degranulation is an additional source of enzymuria in the primary i.e. glomerular inflammatory process. Significant relationship between EL excretion and serum creatinine may indicate that EL released from PMN may also participate in the secondary i.e. interstitial injury that is decisive in the progression of GN.

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Section: Methodsmentioning

confidence: 99%

Etiology of Increased Enzymuria in Different Morphological Forms of Glomerulonephritis

Kuźniar¹,

Marchewka²,

Lembas-Bogaczyk³

et al. 2004

Nephron Physiol

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“…In adults, physio logical NAG enzymuria only includes low amounts of NAGB [Gibey et al, 1984], Enhanced urinary excretion of NAG in adults can be accompanied by a marked excretion of NAGB with [Ellis et al, 1975] or without [Paraire et al, 1983] correlation to the level of NAGA activity. In normal neonates, the NAGB/total NAG ratio varies from 16 to 25% but does not increase during gentamicin treatment, as it does in adults, thus suggesting that gen tamicin tubular effects could be simply detected by total NAG determina tion.…”

Section: Resultsmentioning

confidence: 99%

“…NAGB is presently regarded as a better marker of lysosomal injury than total NAG in adult patients [Tucker et al, 1980;Paraire et al, 1983], because the former is strongly linked to lysosomal membrane whereas NAGA (the other main isoenzymatic form of NAG) is part of the soluble intralysosomal compartment [Bourbouze et al, 1984]. In adults, physio logical NAG enzymuria only includes low amounts of NAGB [Gibey et al, 1984], Enhanced urinary excretion of NAG in adults can be accompanied by a marked excretion of NAGB with [Ellis et al, 1975] or without [Paraire et al, 1983] correlation to the level of NAGA activity.…”

Section: Resultsmentioning

confidence: 99%

Urinary Excretion of N-Acetyl-Glucosaminidase and Beta-2-Microglobulin as Early Markers of Gentamicin Nephrotoxicity in Neonates

Jb¹,

Aujard²,

Abisror³

et al. 1987

Dev Pharmacol Ther

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A prospective study in 16 healthy and 16 gentamicin-treated neonates was undertaken to compare the urinary excretion of proximal tubular markers such as β(2)- microglobulin (β(2)-m) and total N-acetyl-β-D-glucosammidase (NAG) and its isoenzymatic form NAGB. β(2)-m excretion was not related to postnatal age in control full-term neonates; it was significantly increased in gentamicin-treated full-term neonates. The urinary excretion of the lysosomal markers, total NAG and NAGB, rose significantly with postnatal age in control group. fMean values for total NAG and NAGB were significantly higher in the treated group but the isoenzymatic profile (NAGB/total NAG × 100) was not modified by gentamicin treatment.

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“…For this purpose, an original method of measurement was used for monitoring the B isoenzyme excretion in addition to tlie N-acetyl-ß-/)-glucosaminidase (A + B) total enzymuria (4).…”

Section: Introductionmentioning

confidence: 99%

Excretion of Urinary N-Acetyl-ß-D-glucosaminidase Isoenzymes after Renal Transplantation in the Rat

Bourbouze¹,

Dubois²,

Gluckman³

et al. 1987

Clinical Chemistry and Laboratory Medicine

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The urinary excretion of N-acetyl-ß-Z)-glucosaminidase isoenzymes A and B following kidney transplantation was studied in rats. High enzymuria with permanent marked isoenzyme B excretion occurred from the immediate post-operative period to the irreversible rejection episode. Isoenzyme B could represent äs much äs 10-40% of total N-acetyl-ß-jD-glucosaminidase activity and it reflected the intensity of tubular lesions äs observed by histological examination of allograft specimens. Thus, N-acetyl-ß-J9-glucosaminidase B isoenzyme determination may reinforce the diagnostic value of total (A 4-B) urinary N-acetyl-ß-Z>-glucosaminidase activity determination during the various complications which can occur after transplantation.

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Differential assay of A and B isoenzymes in urinary N-acetyl-β-d-glucosaminidase

Cited by 20 publications

References 15 publications

Etiology of Increased Enzymuria in Different Morphological Forms of Glomerulonephritis

Etiology of Increased Enzymuria in Different Morphological Forms of Glomerulonephritis

Urinary Excretion of N-Acetyl-Glucosaminidase and Beta-2-Microglobulin as Early Markers of Gentamicin Nephrotoxicity in Neonates

Excretion of Urinary N-Acetyl-ß-D-glucosaminidase Isoenzymes after Renal Transplantation in the Rat

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