Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

“…The inclusion body was suspended by electrophoresis 2× loading buffer (0.1 M Tris-HCl, pH 6.8, 20% glycerol, 4% SDS, 0.005% Bromophenol Blue, 10% 2-mercaptoethanol), and a total of 4 mg protein of the expressed fusion polypeptide Trx-AsGTH-␣ I, Trx-AsFSH␤ and Trx-AsLH␤ was, respectively, isolated and purified by SDS-PAGE electrophoresis as described previously (Dong et al, 2004). Then, the fusion protein was directly excised from the gel (Dong et al, 2004) and used to prepare polyclonal antibodies by immunizing rabbit or mouse as described previously (Xia et al, 2007).…”

Molecular mechanisms underlying sex change in hermaphroditic groupers

“…Recently, many researchers constructed a set of SMART (switching mechanism at 5 0 end of the RNA transcript) cDNA plasmid libraries from orange-spotted groupers' pituitaries, hypothalamus and gonads at different stages and initiated a series of molecular studies in order to reveal the regulative mechanisms of growth, development, reproduction, and sex change (Jiang et al 2003;Yao et al 2003Yao et al , 2007Jia et al 2004a, b;Wang et al 2004;Zhang et al 2004aZhang et al , b, c, 2007Chen et al 2005;Li et al 2005bLi et al , c, 2007aYe et al 2005;Zhou et al 2005Zhou et al , 2006Zhou et al , 2007Ji et al 2006;Cui et al 2007;Feng et al 2007;Xia et al 2007). To date, we have cloned and identified approximately ten genes related to reproduction regulation or sex differentiation in groupers (Table 2) and analysed their dynamic expression changes and cellular distribution.…”

Evolutionary history of two divergent Dmrt1 genes reveals two rounds of polyploidy origins in gibel carp

“…Dmrt1 (dsx and mab-3 related transcription factor 1) is a member of a gene family of putative transcription factors that share a highly conserved DNA binding domain (DM domain) believed to play critical roles in testis development and differentiation of vertebrates (Herpin and Schartl, 2011;Masuyama et al, 2012;Xia et al, 2007;Zarkower, 2013). Several sex determination and sex chromosome-linked genes in vertebrates such as Dmy in medaka (Matsuda et al, 2002;Nanda et al, 2002), DM-W in the W chromosome of African clawed frog (Yoshimoto et al, 2008), and Dmrt1 in the Z chromosome of birds (Smith et al, 2009), have been shown to be recently duplicated homologs of Dmrt1.…”

“…Several sex determination and sex chromosome-linked genes in vertebrates such as Dmy in medaka (Matsuda et al, 2002;Nanda et al, 2002), DM-W in the W chromosome of African clawed frog (Yoshimoto et al, 2008), and Dmrt1 in the Z chromosome of birds (Smith et al, 2009), have been shown to be recently duplicated homologs of Dmrt1. Because of the high evolutionary conservation and the newly recruited role in vertebrates (Herpin and Schartl, 2011;Xia et al, 2007), Dmrt1 also allows us to analyze the evolutionary history of genome duplication through examination of divergence and phylogeny of the duplicated genes. In this study, we analyzed Dmrt1 genes of polyploid gibel carp and closely related species to reveal genetic evidence for supporting hypotheses of the polyploidy origins.…”