Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus

Thio, Christina Li‐Ping; Yusof, Rohana; Ashrafzadeh, Ali; Bahari, Syareena; Abdul‐Rahman, Puteri Shafinaz; Karsani, Saiful Anuar

doi:10.1371/journal.pone.0129033

Cited by 13 publications

(10 citation statements)

References 37 publications

(43 reference statements)

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“… 79 Secretomes analysis also identifies the proteins in CM, which can be used as biomarker identification for specific diseases and in drug identification and development. 80 Therefore, studies on secretomes provide insight into tissue engineering and regenerative medicine.…”

Section: Secretomesmentioning

confidence: 99%

Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

et al. 2022

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Wound represents a significant socioeconomic burden for both affected individuals and as a whole healthcare system. Accordingly, stem cells have garnered attention due to their differentiation capacity and ability to aid tissue regeneration by releasing biologically active molecules, found in the cells’ cultivated medium which known as conditioned medium (CM) or secretomes. This acellular approach provides a huge advantage over conventional treatment options, which are mainly used cellular treatment at wound closure. Interestingly, the secretomes contained the cell-secreted proteins such as growth factors, cytokines, chemokines, extracellular matrix (ECM), and small molecules including metabolites, microvesicles, and exosomes. This review aims to provide a general view on secretomes and how it is proven to have great potential in accelerating wound healing. Utilizing the use of secretomes with its secreted proteins and suitable biomaterials for fabrications of acellular skin substitutes can be promising in treating skin loss and accelerate the healing process.

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Section: Secretomesmentioning

confidence: 99%

Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

et al. 2022

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“…Early evidence indicated that in different human pathologies the cellular dysfunction of different cell types including dermal fibroblasts leads to an aberrant secretome that reprograms the microenvironment and induces myofibroblast differentiation [15,16]. Hence, in pathologic conditions the secretome changes may serve to disclose disease-specific biomarkers with a possible therapeutic relevance [14,17].…”

Section: Introductionmentioning

confidence: 99%

Matrix Metalloproteinases Inhibition by Doxycycline Rescues Extracellular Matrix Organization and Partly Reverts Myofibroblast Differentiation in Hypermobile Ehlers-Danlos Syndrome Dermal Fibroblasts: A Potential Therapeutic Target?

Chiarelli

Zoppi

Venturini

et al. 2021

Cells

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Hypermobile Ehlers-Danlos syndrome (hEDS) is the most frequent type of EDS and is characterized by generalized joint hypermobility and musculoskeletal manifestations which are associated with chronic pain, and mild skin involvement along with the presence of more than a few comorbid conditions. Despite numerous research efforts, no causative gene(s) or validated biomarkers have been identified and insights into the disease-causing mechanisms remain scarce. Variability in the spectrum and severity of symptoms and progression of hEDS patients’ phenotype likely depend on a combination of age, gender, lifestyle, and the probable multitude of genes involved in hEDS. However, considering the clinical overlap with other EDS forms, which lead to abnormalities in extracellular matrix (ECM), it is plausible that the mechanisms underlying hEDS pathogenesis also affect the ECM to a certain extent. Herein, we performed a series of in vitro studies on the secretome of hEDS dermal fibroblasts that revealed a matrix metalloproteinases (MMPs) dysfunction as one of the major disease drivers by causing a detrimental feedback loop of excessive ECM degradation coupled with myofibroblast differentiation. We demonstrated that doxycycline-mediated inhibition of MMPs rescues in hEDS cells a control-like ECM organization and induces a partial reversal of their myofibroblast-like features, thus offering encouraging clues for translational studies confirming MMPs as a potential therapeutic target in hEDS with the expectation to improve patients’ quality of life and alleviate their disabilities.

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“…The non-correlation between transcription levels and amounts of secreted protein indicates that secretion is regulated by some not-yet disclosed mechanism, involving transcription, translation, storage, sorting, and transport, as already reported for other systems [ 91 ]. This non-correlation was also observed when the exoproteome of WRL-68 human hepatic cells infected with the chikungunya virus was analyzed [ 92 ].…”

Section: Resultsmentioning

confidence: 73%

Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response

Martins-da-Silva

Telleria

Batista

et al. 2018

Viruses

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Hematophagous insects transmit infectious diseases. Sand flies are vectors of leishmaniasis, but can also transmit viruses. We have been studying immune responses of Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. We identified a non-specific antiviral response in L. longipalpis LL5 embryonic cells when treated with non-specific double-stranded RNAs (dsRNAs). This response is reminiscent of interferon response in mammals. We are investigating putative effectors for this antiviral response. Secreted molecules have been implicated in immune responses, including interferon-related responses. We conducted a mass spectrometry analysis of conditioned medium from LL5 cells 24 and 48 h after dsRNA or mock treatment. We identified 304 proteins. At 24 h, 19 proteins had an abundance equal or greater than 2-fold change, while the levels of 17 proteins were reduced when compared to control cells. At the 48 h time point, these numbers were 33 and 71, respectively. The two most abundant secreted peptides at 24 h in the dsRNA-transfected group were phospholipid scramblase, an interferon-inducible protein that mediates antiviral activity, and forskolin-binding protein (FKBP), a member of the immunophilin family, which mediates the effect of immunosuppressive drugs. The transcription profile of most candidates did not follow the pattern of secreted protein abundance.

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Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus

Cited by 13 publications

References 37 publications

Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products

Matrix Metalloproteinases Inhibition by Doxycycline Rescues Extracellular Matrix Organization and Partly Reverts Myofibroblast Differentiation in Hypermobile Ehlers-Danlos Syndrome Dermal Fibroblasts: A Potential Therapeutic Target?

Identification of Secreted Proteins Involved in Nonspecific dsRNA-Mediated Lutzomyia longipalpis LL5 Cell Antiviral Response

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