Differential Analysis of lncRNA, miRNA and mRNA Expression Profiles and the Prognostic Value of lncRNA in Esophageal Cancer

Liu, Hongtao; Zhang, Qing; Lou, Qianqian; Zhang, Xin; Cui, Yunxia; Wang, Panpan; Yang, Fan; Wu, Fan; Wang, Jing; Fan, Tingting; Li, Shenglei

doi:10.1007/s12253-019-00655-8

Cited by 64 publications

(60 citation statements)

References 43 publications

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“…The present study used TCGA data and three GEO datasets to identify common dysregulated miRNAs in GC. A similar analysis has also been reported by a number of other groups (16)(17)(18)(19).…”

Section: Methodssupporting

confidence: 86%

Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

Yuan

Zhang

et al. 2019

Oncol Lett

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Gastric cancer is one of the most common malignancies in China and exhibits a poor prognosis. The most significant challenge for gastric cancer treatment is the absence of early diagnostic biomarkers. MicroRNAs (miRNAs) are small non-coding RNAs, which possess clinical value in a number of different types of cancer. The current study identified 13 miRNAs (hsa-miR-22, hsa-miR-545, hsa-let-7i, hsa-miR-15b, hsa-miR-221, hsa-miR-196a, hsa-miR-20a, hsa-miR-196b, hsa-miR-93, hsa-miR-19a, hsa-miR-503, hsa-miR-106b and hsa-miR-18a) that were significantly overexpressed in GC, by analyzing 1,000 GC samples included in four public datasets, including GSE23739, GSE78091, GSE30070 and The Cancer Genome Atlas. Furthermore, it was revealed that the expression levels of these 13 miRNAs were significantly higher in gastric cancer tissues of grades I, II and III compared with normal controls. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis demonstrated that the differentially expressed miRNAs were involved in regulating transcription, protein amino acid phosphorylation, signal transduction, protein binding, zinc ion binding, the mitogen-activated protein kinase signaling pathway and focal adhesion. In summary, the present study may provide potential new therapeutic and prognostic targets for gastric cancer.

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Section: Methodssupporting

confidence: 86%

Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

Yuan

Zhang

et al. 2019

Oncol Lett

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“…Downregulation of WDFY3-AS2 expression has been reported in different types of tumors, including breast cancer [50][51][52]. WDFY3-AS2 was found down-regulated in glioma and was associated with poor patient survival [51].…”

Section: Plos Onementioning

confidence: 96%

In silico analyses identify lncRNAs: WDFY3-AS2, BDNF-AS and AFAP1-AS1 as potential prognostic factors for patients with triple-negative breast tumors

Bastos

Nagai

2020

PLoS ONE

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Background Long non-coding RNAs (lncRNAs) are characterized as having 200 nucleotides or more and not coding any protein, and several been identified as differentially expressed in several human malignancies, including breast cancer. Methods Here, we evaluated lncRNAs differentially expressed in triple-negative breast cancer (TNBC) from a cDNA microarray data set obtained in a previous study from our group. Using in silico analyses in combination with a review of the current literature, we identify three lncRNAs as potential prognostic factors for TNBC patients. Results We found that the expression of WDFY3-AS2, BDNF-AS, and AFAP1-AS1 was associated with poor survival in patients with TNBCs. WDFY3-AS2 and BDNF-AS are lncRNAs known to play an important role in tumor suppression of different types of cancer, while AFAP1-AS1 exerts oncogenic activity. Conclusion Our findings provided evidence that WDFY3-AS2, BDNF-AS, and AFAP1-AS1 may be potential prognostic factors in TNBC development.

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“…18 Many lncRNAs play pivotal roles in maintaining and promoting the biological characteristics of tumour cells, and thus, lncRNAs may be the attractive therapeutic targets in a variety of tumours. 19,20 Our recent work identified many differential lncRNA through TCGA database, and we revealed that ZEB1-AS1 was significantly up-regulated in ESCA, 21 but its precise functions remain unknown. It is a fact that ZEB1-AS1 is closely correlated with tumour occurrence and development, such as bladder cancer, 22 glioma, 23 melanoma 24 and non-small-cell lung cancer, 25 and these data suggest that ZEB1-AS1 is involved in tumour progression via multiple different molecular mechanisms, suggesting the complexity of ZEB1-AS1 function in these tumours.…”

mentioning

confidence: 89%

LncRNA ZEB1‐AS1 down‐regulation suppresses the proliferation and invasion by inhibiting ZEB1 expression in oesophageal squamous cell carcinoma

Zhao

Wang

Zhang

et al. 2019

J Cellular Molecular Medi

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Multiple studies have unveiled that long non‐coding RNAs (lncRNAs) play a pivotal role in tumour progression and metastasis. However, the biological role of lncRNA ZEB1‐AS1 in oesophageal squamous cell carcinoma (ESCC) remains under investigation, and thus, the current study was to investigate the functions of ZEB1‐AS1 in proliferation and invasion of ESCC. Here, we discovered that ZEB1‐AS1 and ZEB1 were markedly up‐regulated in ESCC tissues and cells relative to their corresponding normal control. ZEB1‐AS1 and ZEB1 overexpressions were both related to TNM staging and lymph node metastasis as well as poor prognosis in ESCC. The hypomethylation of ZEB1‐AS1 promoter triggered ZEB1‐AS1 overexpression in ESCC tissues and cells. In addition, ZEB1‐AS1 knockdown mediated by siRNA markedly suppressed the proliferation and invasion in vitro in EC9706 and TE1 cells, which was similar with ZEB1 siRNA treatment, coupled with EMT alterations including the up‐regulation of E‐cadherin level as well as the down‐regulation of N‐cadherin and vimentin levels. Notably, ZEB1‐AS1 depletion dramatically down‐regulated ZEB1 expression in EC9706 and TE1 cells, and ZEB1 overexpression obviously reversed the inhibitory effects of proliferation and invasion triggered by ZEB1‐AS1 siRNA. ZEB1‐AS1 shRNA evidently inhibited tumour growth and weight, whereas ZEB1 elevation partly recovered the tumour growth in ESCC EC9706 and TE1 xenografted nude mice. In conclusion, ZEB1‐AS1 overexpression is tightly involved in the development and progression of ESCC, and it exerts the antitumour efficacy by regulating ZEB1 level in ESCC.

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Differential Analysis of lncRNA, miRNA and mRNA Expression Profiles and the Prognostic Value of lncRNA in Esophageal Cancer

Cited by 64 publications

References 43 publications

Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

In silico analyses identify lncRNAs: WDFY3-AS2, BDNF-AS and AFAP1-AS1 as potential prognostic factors for patients with triple-negative breast tumors

LncRNA ZEB1‐AS1 down‐regulation suppresses the proliferation and invasion by inhibiting ZEB1 expression in oesophageal squamous cell carcinoma

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