Differential Ammonia-Elicited Changes of Cytosolic pH in Root Hair Cells of Rice and Maize as Monitored by 2[prime],7[prime]-bis-(2-Carboxyethyl)-5 (and -6)-Carboxyfluorescein-Fluorescence Ratio

Kosegarten, Harald; Grolig, Franz; Wieneke, J.; Wilson, G.; Hoffmann, Bernd

doi:10.1104/pp.113.2.451

Cited by 59 publications

(47 citation statements)

References 43 publications

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“…The influx and assimilation of nitrate and ammonium requires cytosolic pH homeostasis, with regulation partly contributed by the OsNRT2.3b sensing motif. The protoncotransport mechanism for the entry of nitrate into cells provides cytosolic acidification, whereas ammonium transport can cause an alkalinization (34), which may enhance proton-coupled nitrate transport. The assimilation of ammonium produces at least one H + per NH + 4 , whereas NO − 3 assimilation produces almost one OH − per NO − 3 (32).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Fan

Tang

Tan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

324

234

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Fig. 3. Growth, yield, and NUE of OsNRT2.3b, OsNRT2.3a, and H167R mutation overexpressing lines. (A) Phenotypes and transcriptional and translational expression of OsNRT2.3b-and OsNRT2.3a-overexpressing lines and Nipponbare WT. (B) Phenotypes and transcriptional and translational expression of WT and OsNRT2.3b-H167R mutant-overexpressing lines. (C) Average grain yield and NUE of OsNRT2.3b-(O), OsNRT2.3a-(a-O), and H167R-(H167R) overexpressing lines and WT in field plots. RT-PCR with the specific primers (SI Appendix, Table S10) and Western blot analyses with monoclonal antibodies were performed to identify protein expression levels. NUE = grain yield/applied N fertilizer. Values are mean ± SE (n = 3). a and b above bars indicate significant differences (P < 0.05) between the transgenic lines and WT estimated by one-way ANOVA.

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Section: Discussionmentioning

confidence: 99%

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Fan

Tang

Tan

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

324

234

View full text Add to dashboard Cite

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“…Published data reveal that low-affinity putative 13 NH 4 ϩ fluxes declined from 18.63 to 11.44 mol g Ϫ1 h Ϫ1 as external pH increased from 4.5 to 7.5 in rice roots (Wang et al, 1993b). Thus, despite a 1,000-fold increase of [ 13 NH 3 ] o , tracer influx actually declined by 40%; a similar decline of 13 NH 4 ϩ fluxes between pH 5 and 7, was observed by Kosegarten et al (1997) Figure 1A, the gradient for NH 3 permeation across the tonoplast is in the opposite direction (from vacuole to cytosol), requiring active transport of NH 3 to the vacuole. The data reported by Lee and Ratcliffe (1991), Roberts and Pang (1992), and Wang et al (1993a) provide [NH 4 ϩ ] v values that are Ͼ1 mm.…”

Section: Plasma-membrane Fluxes Of Nh 4 ؉ /Nh 3 : a Thermodynamic Evamentioning

confidence: 69%

Cytosolic Concentrations and Transmembrane Fluxes of NH4 +/NH3. An Evaluation of Recent Proposals

et al. 2001

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“…Such an unexpectedly rapid return to a set¯ux equilibrium emphasises the ®delity of the control mechanism(s) integrating N¯uxes at the cellular level, which is only gradually transduced into a whole-organism response. Transient perturbations in other key cellular parameters, such as membrane electrical potential and cytosolic pH, are also known to rapidly follow changes in extracellular N provision of the magnitude imposed here (Wang et al 1994;Kosegarten et al 1997), suggesting that they might be involved in the recti®cation of N-turnover kinetics. However, in many cases these processes require signi®-cantly longer times to achieve new resting states, when compared to k c recti®cation (Wang et al 1994;Kosegarten et al 1997).…”

Section: Compartmental Analysismentioning

confidence: 98%

Constancy of nitrogen turnover kinetics in the plant cell: insights into the integration of subcellular N fluxes

Britto

Kronzucker

2001

Planta

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Compartmental analysis with 13N was used to determine cytosolic nitrate (NO3-) pools, and their turnover rates, in roots of intact barley (Hordeum vulgare L. cv Klondike) seedlings. Influx, efflux, flux to the vacuole and assimilation, and flux to the xylem, varied as much as 300-fold over a wide range of external NO3- conditions. By contrast, the kinetic constant kc describing cytosolic NO3- turnover varied by less than 4% from a mean value of 0.0407 min(-1). Accordingly, cytosolic NO3- pools varied linearly with influx. A literature survey showed that kc constancy is observed with both NO3- and ammonium (NH4+) fluxes in many plant species, including H. vulgare, Arabidopsis thaliana, Picea glauca, and Oryza sativa. The regulatory system implied by this phenomenon is fundamentally different from that of potassium (K+) fluxes, in which cytosolic pool size is held constant while kc varies with external K+ concentrations. We further present data showing that barley plants, grown on one steady-state concentration of NH4+, restore kc within minutes of exposure to new, non-steady-state, NH4+ concentrations. We propose the existence of a high-fidelity mechanism governing the timing of cytosolic N turnover, and discuss its implications for attempts to improve plants biotechnologically.

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Differential Ammonia-Elicited Changes of Cytosolic pH in Root Hair Cells of Rice and Maize as Monitored by 2[prime],7[prime]-bis-(2-Carboxyethyl)-5 (and -6)-Carboxyfluorescein-Fluorescence Ratio

Cited by 59 publications

References 43 publications

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields

Cytosolic Concentrations and Transmembrane Fluxes of NH4 +/NH3. An Evaluation of Recent Proposals

Constancy of nitrogen turnover kinetics in the plant cell: insights into the integration of subcellular N fluxes

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