Differential adhesiveness between blood and marrow leukemic cells having similar pattern of VLA adhesion molecule expression

Thomas, Xavier; Annibale, Bruno; Bailly, Mireille; O, Maritaz; Magaud, Jean‐Pierre; Archimbaud, E

doi:10.1016/s0145-2126(98)00103-9

Cited by 14 publications

(16 citation statements)

References 23 publications

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“…A previous study conducted by Ma et al showed that E‐selectin could significantly increase capture capacity of bone marrow HSCs on NFS compared with unmodified nanofibers and TCP. Our findings are comparable to those previously reported . Coating of ECM proteins will not only help to improve cell adhesion but also serves to improve overall biocompatibility of the material itself.…”

Section: Discussionsupporting

confidence: 91%

“…The expression of integrins‐α2 and ‐α5 is generally high on cultured K562 cells > 95% on both captured and noncaptured K562 cell populations. As we observed in this study and with others, high expressions of integrins do not represent functional adhesive nature especially on leukemic cells . It has also been observed that the inability of leukemic hematopoietic cells to interact with the bone marrow stroma and ECM counterparts alters their functional behaviors .…”

Section: Discussionsupporting

confidence: 55%

“…It has been proposed that increased proliferation and resistance to apoptosis of CML cells in the bone marrow is a consequence of altered adhesive behaviors of leukemic progenitors, which results in their lack of responsiveness to negative regulatory mechanisms . Several observations in vitro have accounted for abnormal adhesive properties in CML progenitors . The inability for CML progenitors to adhere to bone marrow stroma and ECM proteins such as fibronectin could be responsible for the uncontrolled immature cell expansion .…”

Section: Introductionmentioning

confidence: 99%

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Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

Cao

Kwek

Chan

et al. 2013

J Biomedical Materials Res

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This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell-cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

Cao

Kwek

Chan

et al. 2013

J Biomedical Materials Res

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“…Most likely, these and other factors interact with each other in a complex fashion to determine which blasts will circulate and which will stay in BM. [10][11][12][13] Irrespective of the cause, an increased tendency of blasts to circulate, when defined as the presence of higher blast percentages in PB than in BM, has prognostic value in ALL and MDS. The independent prognostic value of this phenomenon highlights the importance of exploring the causes behind it, and suggests that investigating its underlying causes may help in devising therapeutic strategies.…”

Section: P-valuementioning

confidence: 99%

Having a higher blast percentage in circulation than bone marrow: clinical implications in myelodysplastic syndrome and acute lymphoid and myeloid leukemias

et al. 2005

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Determining the percentage of peripheral blood (PB) and bone marrow (BM) blasts is important for diagnosing and classifying acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Although most patients with acute leukemia or MDS have a higher percentage of BM blasts than PB blasts, the relative proportion is reversed in some patients. We explored the clinical relevance of this phenomenon in MDS (n ¼ 446), AML (n ¼ 1314), and acute lymphoblastic leukemia (ALL) (n ¼ 385). Among patients with MDS or ALL, but not AML, having a higher blast percentage in PB than in BM was associated with significantly shorter survival. In multivariate analyses, these associations were independent of other relevant predictors, including cytogenetic status. Our findings suggest that MDS and ALL patients who have a higher percentage of PB blasts than BM blasts have more aggressive disease. These data also suggest that MDS classification schemes should take into account the percentage of blasts in PB differently from the percentage of blasts in BM.

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“…Little is known about the exact factors that control the mobilization of bone marrow blasts into the PB, although adhesion molecules, chemokines, and angiogenic factors are believed to play a role. [26][27][28] WBC count apparently reflects tumor load only when PB blast count is high, and the number of nonmalignant leukocytes may be altered by infections. 29 To date, the relation between peripheral blood blast cell and prothrombotic factors in childhood ALL are few and results are contradictory.…”

Section: Discussionmentioning

confidence: 99%

Von Willebrand Factor and Factor VIII Levels in Egyptian Children With Newly Diagnosed Acute Lymphoblastic Leukemia in Relation to Peripheral Blast Cells and Steroid Therapy

Sherif

Narouz

Elkerdany

et al. 2014

Journal of Pediatric Hematology/Oncology

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Differential adhesiveness between blood and marrow leukemic cells having similar pattern of VLA adhesion molecule expression

Cited by 14 publications

References 23 publications

Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

Having a higher blast percentage in circulation than bone marrow: clinical implications in myelodysplastic syndrome and acute lymphoid and myeloid leukemias

Von Willebrand Factor and Factor VIII Levels in Egyptian Children With Newly Diagnosed Acute Lymphoblastic Leukemia in Relation to Peripheral Blast Cells and Steroid Therapy

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