Differential Adhesion of Metastatic Rat Mammary Carcinoma Cells to Organ-Derived Microvessel Endothelial Cells and Subendothelial Matrix

Abstract

The in vitro adhesion rates of rat 13762NF mammary adenocarcinoma cell clones of different spontaneous metastatic potentials to cloned microvessel endothelial cell monolayers and their subendothelial extracellular matrix were investigated. In this system, high rates of adhesion of the cloned tumor cell lines to syngeneic target (lung) organ-derived subendothelial matrix correlated with spontaneous metastatic potential, whereas adhesion to the lung microvessel endothelial cell apical surfaces occurred at lower rates and was not highly significantly different among the tumor cell lines. Adhesion rates to bovine aortic large vessel, and human brain and human meningeal microvessel endothelial cell monolayers were, in general, lower than those found with syngeneic lung microvessel endothelial cells, and did not correlate with spontaneous metastatic potential. Growth of endothelial cells in fetal bovine serum or platelet-poor horse serum did not affect the results, suggesting that in this system metastasis-associated organ-adhesive specificity is determined at the level of the subendothelial matrix.

Purification and characterization of a Mr ∼66,000 lung‐derived (paracrine) growth factor that preferentially stimulates the in vitro proliferation of lung‐metastasizing tumor cells

“…The ability to respond to an organ-derived growth factor, however, would only be one of several important properties required for metastasis formation in that organ. For example, lung-metastasizing cells may also require adhesion to lung microvessel endothelial cells [6,7,17,38 J and subendothelial matrix [6,7,38 J.…”

The role of both specific cellular adhesion and growth promotion in liver colonization by F9 embryonal carcinoma cells

“…The most striking result of the experiments shown in Figure 2 was the very low affinity of F9 cells for the ECM of the lungs. Specific binding to the subendothelial matrix may also be involved in determining the organ specificity of metastasis (Lichtner et al, 1989), and the clonal growth of tumor cells on tissue-specific biomatrices correlates with specific organ colonization (Doerr et al, 1989). This, however, does not seem to be the case for F9 cells since, after low-density plating, they grow at the same rate on both lung-and liver-derived ECM (not shown).…”

Cell Surface Molecules and Their Prognostic Values in Assessing Colorectal Carcinomas

“…46 Previous studies have demonstrated a correlation between the organ preference of metastasis and the in vitro adhesion rates of malignant cells to various endothelial cells 47,48 or subendothelial ECM. 49 Using colon carcinoma cell lines with different metastatic properties, we have shown that metastatic behavior correlates with different integrin-mediated adhesive properties. 50,51 However, differences in integrin expression could not explain these differences.…”