Differential Adherence and Expression of Virulence Traits by Candida albicans and Candida parapsilosis in Mono- and Dual-Species Cultures in Artificial Saliva

Abstract

To understand the expression of virulence factors in Candida dual-species systems.

Oral yeast colonization in patients with eating disorders: commensal acquisition or due to purgative habits?

“…We observed that C. parapsilosis was found in association with C. albicans. Seabra et al 22 reported that both strains, when isolated from cocultures on artificial saliva showed higher adherence and virulence traits in vitro than strains from monocultures.…”

“…The MALDI-TOF MS technique for bacteria and fungi identification up to the genus and species level has been causing a makeover to the workflows for the identification of many pathogens. This technique emerges as a fast, accurate and cost-effective option to replace and/or to complement the conventional phenotypic identification 22 . Additionally, the ability of MALDI-TOF MS to detect a large array of proteins allows the discrimination of closely related species of organisms 25 .…”

Avaliação de fatores de virulência e sensibilidade a antissépticos bucais de espécies de Candida isoladas da saliva de pacientes com próteses bucais

“…Os produtos de PCR foram analisados após a corrida eletroforética a 90 Volt, 110 miliampère durante 2h em gel de agarose a 1%, TBE 0,5X preparado em paralelo com o padrão de peso molecular 100pb. Ao termino da corrida ensaio foi realizado com monoculturas e culturas mistas proveniente do mesmo paciente(SEABRA et al, 2013). Os biofilmes de culturas mistas foram realizados adicionando partes iguais de cada um dos respectivos inóculos e a seguir foi realizado procedimento como descrito acima.…”