Differential adaptation of metabolic inflammation between primiparous and multiparous Zebu cows during transition period

Abstract

This study aimed to estimate the changes in the milk yield, milk fat, energy indicators [NEFA, BHBA, Dry MatterIntake (DMI) and Body Condition Score (BCS)] and concentration of innate immune molecules (Haptoglobin: Hp,Serum Amyloid A: SAA, TLR-4, TNF-α, IL-1β, IL-6, and IL-8), during the transition period in primiparous andmultiparous dual-purpose zebu (Deoni) cows. The blood sample was collected at weekly intervals during pre-partum (-21±2, -14±1, -7±1, d), date of calving (day 0), and postpartum period (3±1, 7±1, 14±1, 21±2 d) for estimationof the above plasma variables using commercially available bovine specific ELISA kits. DMI and BCS duringthe corresponding period were also recorded. Data were analyzed using a linear mixed model considering group,time and their interaction as fixed effects. Group, time and their interaction had significant effect on DMI whereprimiparous cows consumed higher DMI during early postpartum period as compared to multiparous cows. Groupalone had significant effect on milk yield, milk fat per cent and BHBA level while time alone influenced BCS.The interaction of group and time had significant effects on plasma TLR-4 and IL-8 concentration. Group alsohad significant effect on Hp and TNF-α levels. It was concluded that parity had significant effect on metabolicand immune indicators where higher DMI during transition period resulted in more milk yield in primiparous thanmultiparous indigenous (Deoni) cows.