Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils

Valenzuela, Susana V.; Valls, Cristina; Schink, Viviane; Sánchez, D. Mata; Roncero, M. Blanca; Dı́az, Pilar; Martínez, Josefina; Pastor, F. I. Javier

doi:10.1016/j.carbpol.2018.11.076

Cited by 41 publications

(46 citation statements)

References 47 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Even using a mild mechanical treatment consisting of dispersion and sonication, cellulose fibers were disrupted down to nanoscale. Subsequent studies performed by Valenzuela et al [28] investigated the synergy between bacterial LPMOs and endoglucanases followed by high-pressure homogenization for the production of NFC from flax pulp. The authors found significantly higher yields when both enzymes were combined (24.3%) compared to pure LPMO (12.7%) or monocomponent endoglucanase (17.0%) alone.…”

Section: Discussionmentioning

confidence: 99%

“…These enzymes have been initially used in synergy with glycoside hydrolases to boost the saccharification of plant biomass, and they have been incorporated in last-generation commercial enzyme cocktails for the production of biofuels [27]. Recent studies have shown that LPMOs may be used in synergy with cellulases and/or xylanases to facilitate the deconstruction of cellulose fibers in an attempt to produce NFC [28, 29]. In that line of research, we had previously demonstrated that monocomponent LPMOs can disrupt the cellulose fibers structure by the creation of nicking points that weaken the fiber cohesion [30].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production

Moreau

Tapin-Lingua²,

Grisel

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that cleave polysaccharides through an oxidative mechanism. These enzymes are major contributors to the recycling of carbon in nature and are currently used in the biorefinery industry. LPMOs are commonly used in synergy with cellulases to enhance biomass deconstruction. However, there are few examples of the use of monocomponent LPMOs as a tool for cellulose fibrillation. In this work, we took advantage of the LPMO action to facilitate disruption of wood cellulose fibers as a strategy to produce nanofibrillated cellulose (NFC). Results The fungal LPMO from AA9 family ( Pa LPMO9E) was used in this study as it displays high specificity toward cellulose and its recombinant production in bioreactor is easily upscalable. The treatment of birchwood fibers with Pa LPMO9E resulted in the release of a mixture of C1-oxidized oligosaccharides without any apparent modification in fiber morphology and dimensions. The subsequent mechanical shearing disintegrated the LPMO-pretreated samples yielding nanoscale cellulose elements. Their gel-like aspect and nanometric dimensions demonstrated that LPMOs disrupt the cellulose structure and facilitate the production of NFC. Conclusions This study demonstrates the potential use of LPMOs as a pretreatment in the NFC production process. LPMOs weaken fiber cohesion and facilitate fiber disruption while maintaining the crystallinity of cellulose. Electronic supplementary material The online version of this article (10.1186/s13068-019-1501-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production

Moreau

Tapin-Lingua²,

Grisel

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…The column was reconditioned between each run by running initial conditions for 10 min. Standards were generated using 1, 2, 4 and 8 μg mL −1 cellobiose and cellobionic acid [17].…”

Section: Methodsmentioning

confidence: 99%

“…The enzymatic effects that LPMO produce in cellulose have been mainly evaluated through their increase in cellulose degradation [13, 14]. However, the effect that LPMO produces on pulp fibres has been poorly investigated [15–17]. These authors demonstrated that LPMO weakens fibres cohesion, promoting their disruption during mechanical fibrillation.…”

Section: Introductionmentioning

confidence: 99%

Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters

Valls

Pastor

Roncero

et al. 2019

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

Background The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically in order to obtain new bioproducts. Refined cotton linters with high cellulose content were treated with hydrolytic (cellulases) and oxidative (LPMO and Laccase_Tempo) enzymes to evaluate their effect on fibre properties and in improving mechanical fibrillation. Results Cellulases released cellooligosaccharides, reducing fibre length and partially degrading cellulose. They also improved mechanical fibrillation yielding up to 18% of nanofibrillated cellulose (NFC). LPMO introduced a slight amount of COOH groups in cellulose fibres, releasing cellobionic acid to the effluents. The action of cellulases was improved after LPMO treatment; however, the COOH groups created disappeared from fibres. After mechanical fibrillation of LPMO–cellulase-treated cotton linters a 23% yield of NFC was obtained. Laccase_Tempo treatment also introduced COOH groups in cellulose fibres from cotton, yielding 10% of NFC. Degree of polymerization was reduced by Laccase_Tempo, while LPMO treatment did not significantly affect it but produced a higher reduction in fibre length. The combined treatment with LPMO and cellulase provided films with higher transparency (86%), crystallinity (92%), smoothness and improved barrier properties to air and water than films casted from non-treated linters and from commercial NFC. Conclusions The combined enzymatic treatment with LPMO and cellulases boosted mechanical fibrillation of cotton linters, improving the NFC production and providing bioproducts with high transparency and high barrier properties. Electronic supplementary material The online version of this article (10.1186/s13068-019-1502-z) contains supplementary material, which is available to authorized users.

show abstract

“…The same effect described by Bernardi et al (2018) occurred [4]. However, considering the important role played by Cu 2+ in lytic polyssacharide monooxygenase (LPMO) activity and the synergistic effect that LPMO and endoglucanases have on biomass hydrolysis, Af-EGL7 activity was also assessed at different Cu 2+ concentrations (Table 2) [23].…”

Section: Resultsmentioning

confidence: 88%

A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

Bernardi

Yonamine

Uyemura

et al. 2019

IJMS

View full text Add to dashboard Cite

In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study aims to improve on the characterization of a thermostable Aspergillus fumigatus endo-1,4-β-glucanase GH7 (Af-EGL7). To this end, Af-EGL7 was successfully expressed in Pichia pastoris X-33. The kinetic parameters Km and Vmax were estimated and suggested a robust enzyme. The recombinant protein was highly stable within an extreme pH range (3.0–8.0) and was highly thermostable at 55 °C for 72 h. Low Cu2+ concentrations (0.1–1.0 mM) stimulated Af-EGL7 activity up to 117%. Af-EGL7 was tolerant to inhibition by products, such as glucose and cellobiose. Glucose at 50 mM did not inhibit Af-EGL7 activity, whereas 50 mM cellobiose inhibited Af-EGL7 activity by just 35%. Additionally, the Celluclast® 1.5L cocktail supplemented with Af-EGL7 provided improved hydrolysis of sugarcane bagasse “in natura”, sugarcane exploded bagasse (SEB), corncob, rice straw, and bean straw. In conclusion, the novel characterization of Af-EGL7 conducted in this study highlights the extraordinary properties that make Af-EGL7 a promising candidate for industrial applications.

show abstract

Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils

Cited by 41 publications

References 47 publications

Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production

Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production

Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters

A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

Contact Info

Product

Resources

About